Effects of the probiotic Bifidobacterium animalis subsp. lactis on the non-surgical treatment of periodontitis. A histomorphometric, microtomographic and immunohistochemical study in rats

Ricoldi, Milla Sprone Tavares; Furlaneto, Flávia Aparecida Chaves; Oliveira, Luana Farias de; Teixeira, G. C.; Pischiotini, Jéssica P.; Moreira, André L.; Ervolino, Edílson; Oliveira, Maricê Nogueira de; Bogsan, Cristina Stewart Bittencourt; Salvador, Sérgio Luiz de Souza; Messora, Michel Reis

doi:10.1371/journal.pone.0179946

Cited by 65 publications

(115 citation statements)

References 35 publications

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“…It is known that IL-1b positively regulates the synthesis of collagenases and prostaglandins and is associated with degradation through proteases, acting directly in the process of inflammation (Bickel et al 2001). Similar results to this study were found when probiotics were used as coadjuvants in the treatment of periodontitis (Messora et al 2016, Ricoldi et al 2017, Kobayashi et al 2017. Similar results to this study were found when probiotics were used as coadjuvants in the treatment of periodontitis (Messora et al 2016, Ricoldi et al 2017, Kobayashi et al 2017.…”

Section: Discussionsupporting

confidence: 86%

“…Therefore, the null hypothesis was rejected. Furthermore, the dose/frequency can also have an influence and 10 9 CFUs were used due to being successful in the coadjuvant treatment of periodontitis (Ricoldi et al (2017). Apical periodontitis was induced by exposing the pulp of rat molars to the oral environment allowing pulp tissue infection and culminating in pulp necrosis and subsequent AP development.…”

Section: Discussionmentioning

confidence: 99%

“…In this study, rats were used as the experimental model because the oral bacterial microflora and the apical response to pulp exposure are similar to those seen in humans (Tani-Ishii et al 1994). The use of noninvasive and natural approaches in the prevention and treatment of oral diseases has been investigated (Ricoldi et al 2017). The 30-day experimental period was chosen because it is considered long enough for chronic AP development (Gomes-Filho et al 2015, Cintra et al 2016, Dal-Fabbro et al 2019).…”

Section: Discussionmentioning

confidence: 99%

“…The control group received 5 mL of water (without probiotics) (Ricoldi et al 2017). According to the manufacturer, 0.07g of Lactobacillus rhamnosus and one capsule of Lactobacillus acidophilus correspond to 10 9 colony-forming units (CFU).…”

Section: Probiotic Therapymentioning

confidence: 99%

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Systemic administration of probiotics reduces the severity of apical periodontitis

Cosme‐Silva¹,

Dal‐Fabbro²,

Cintra³

et al. 2019

Int Endodontic J

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Aim To evaluate the effect of systemic administration of probiotics on the severity of apical periodontitis (AP). Methodology Twenty‐four male Wistar rats were used. AP was induced in the maxillary left/right first molars. The animals were arranged into groups: Control, Lactobacillus rhamnosus, and Lactobacillus acidophilus. Probiotics were administered orally for gavage (109 colony‐forming units diluted in 5 mL of water for 30 days) during the development of AP. After 30 days, cardiac puncture was performed to analyse the complete blood count. Moreover, microbiological analysis of the root canal contents and saliva was performed. Then, the animals were euthanized and the jaw removed for histopathological and IL‐10, IL‐1β and IL‐6 immunolabeling analyses. After the Shapiro–Wilk test of normality, the Kruskal–Wallis followed by Dunn's test was performed for nonparametric data, and analysis of variance followed by the Tukey test was performed for parametric data (P < 0.05). Results No significance difference was observed in the blood profiles and in the counts of microorganisms from the saliva samples among the groups (P > 0.05). Total microorganism counts in the root canal, the inflammatory infiltrate and the immunostaining for IL‐1β and IL‐6 in AP were significantly lower in the probiotic groups when compared with the control group (P < 0.05). IL‐10 was significantly more immunolabled in the probiotic groups than in the control group (P < 0.05). Conclusion Supplementation with probiotics (Lactobacillus rhamnosus and Lactobacillus acidophilus) had a significant effect on the severity of apical periodontitis in rats, demonstrating the anti‐inflammatory effect of probiotics on the development of apical periodontitis.

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Section: Discussionsupporting

confidence: 86%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Probiotic Therapymentioning

confidence: 99%

See 2 more Smart Citations

Systemic administration of probiotics reduces the severity of apical periodontitis

Cosme‐Silva¹,

Dal‐Fabbro²,

Cintra³

et al. 2019

Int Endodontic J

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“…Only two pre‐clinical studies evaluated the effect of bacteria of the genus Bifidobacterium on periodontal disease (Oliveira et al., ; Ricoldi et al., ). Oliveira et al.…”

Section: Introductionmentioning

confidence: 99%

Effects of Bifidobacterium probiotic on the treatment of chronic periodontitis: A randomized clinical trial

Invernici

Salvador

Silva

et al. 2018

J Clinic Periodontology

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179

200

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AimThis randomized placebo‐controlled clinical trial evaluated the effect of Bifidobacterium animalis subsp. lactis (B. lactis) HN019‐containing probiotic lozenges as adjuvant to scaling and root planing (SRP) in patients with generalized chronic periodontitis.Materials and MethodsForty‐one chronic periodontitis patients were recruited and monitored clinically, immunologically, and microbiologically at baseline (before SRP) and 30 and 90 days after SRP. All patients were randomly assigned to a Test (SRP + Probiotic, n = 20) or Control (SRP + Placebo, n = 21) group. The probiotic lozenges were used twice a day for 30 days. The data were statistically analysed.ResultsThe Test group presented a decrease in probing pocket depth and a clinical attachment gain significantly higher than those of the Control group at 90 days. The Test group also demonstrated significantly fewer periodontal pathogens of red and orange complexes, as well as lower proinflammatory cytokine levels when compared to the Control group. Only the Test group showed an increase in the number of B. lactis HN019 DNA copies on subgingival biofilm at 30 and 90 days.ConclusionThe use of B. lactis HN019 as an adjunct to SRP promotes additional clinical, microbiological, and immunological benefits in the treatment of chronic periodontitis (NCT03408548).

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Involvement of the Gut Microbiota and Barrier Function in Glucocorticoid-Induced Osteoporosis

Schepper

Collins

Rios‐Arce

et al. 2019

Journal of Bone and Mineral Research

133

119

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Glucocorticoids (GCs) are potent immune‐modulating drugs with significant side effects, including glucocorticoid‐induced osteoporosis (GIO). GCs directly induce osteoblast and osteocyte apoptosis but also alter intestinal microbiota composition. Although the gut microbiota is known to contribute to the regulation of bone density, its role in GIO has never been examined. To test this, male C57/Bl6J mice were treated for 8 weeks with GC (prednisolone, GC‐Tx) in the presence or absence of broad‐spectrum antibiotic treatment (ABX) to deplete the microbiota. Long‐term ABX prevented GC‐Tx‐induced trabecular bone loss, showing the requirement of gut microbiota for GIO. Treatment of GC‐Tx mice with a probiotic (Lactobacillus reuteri [LR]) prevented trabecular bone loss. Microbiota analyses indicated that GC‐Tx changed the abundance of Verrucomicobiales and Bacteriodales phyla and random forest analyses indicated significant differences in abundance of Porphyromonadaceae and Clostridiales operational taxonomic units (OTUs) between groups. Furthermore, transplantation of GC‐Tx mouse fecal material into recipient naïve, untreated WT mice caused bone loss, supporting a functional role for microbiota in GIO. We also report that GC caused intestinal barrier breaks, as evidenced by increased serum endotoxin level (2.4‐fold), that were prevented by LR and ABX treatments. Enhancement of barrier function with a mucus supplement prevented both GC‐Tx–induced barrier leakage and trabecular GIO. In bone, treatment with ABX, LR or a mucus supplement reduced GC‐Tx–induced osteoblast and osteocyte apoptosis. GC‐Tx suppression of Wnt10b in bone was restored by the LR and high‐molecular‐weight polymer (MDY) treatments as well as microbiota depletion. Finally, we identified that bone‐specific Wnt10b overexpression prevented GIO. Taken together, our data highlight the previously unappreciated involvement of the gut microbiota and intestinal barrier function in trabecular GIO pathogenesis (including Wnt10b suppression and osteoblast and osteocyte apoptosis) and identify the gut as a novel therapeutic target for preventing GIO. © 2019 American Society for Bone and Mineral Research.

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Effects of the probiotic Bifidobacterium animalis subsp. lactis on the non-surgical treatment of periodontitis. A histomorphometric, microtomographic and immunohistochemical study in rats

Cited by 65 publications

References 35 publications

Systemic administration of probiotics reduces the severity of apical periodontitis

Systemic administration of probiotics reduces the severity of apical periodontitis

Effects of Bifidobacterium probiotic on the treatment of chronic periodontitis: A randomized clinical trial

Involvement of the Gut Microbiota and Barrier Function in Glucocorticoid-Induced Osteoporosis

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