Effects of the Tris, Tes, or skim milk based extender on in vitro parameters of ram spermatozoa during liquid storage

Quan, Guo Bo; Wu, Guo Quan; Wang, Ya Jing; Li, Dong Jiang; Ma, Yi; Hong, Qionghua

doi:10.1016/j.smallrumres.2015.11.008

Cited by 18 publications

(13 citation statements)

References 29 publications

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“…Furthermore, we observed that other quality parameters of goat spermatozoa stored at 5 °C, such as velocities and hMMP, also decreased as storage time increased. Recently, other authors also observed a gradual decrease in velocities and hMMP as the length of sperm storage time at 5 °C increased [ 27 , 28 , 29 , 30 , 31 ]. Moreover, Liu et al [ 28 ] also reported the detrimental effect of goat spermatozoa storage at 4 °C in other quality parameters, showing an increase in apoptosis and defects of mitochondria as time increased.…”

Section: Discussionmentioning

confidence: 98%

“…This disagreement could be attributed to the use of different species and methodology, and that the fact that they did not use any CASA system for sperm motility assessment. Moreover, other factors could affect the final results since even the use of different breeds or extenders had an effect on the hMMP of spermatozoa [ 27 , 55 ]. With regard to the effect of sperm concentration on DNA fragmentation, we observed that sDFI was higher when sperm concentration increased.…”

Section: Discussionmentioning

confidence: 99%

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Effect of Sperm Concentration and Storage Temperature on Goat Spermatozoa during Liquid Storage

Sadeghi

Gallego

García-Colomer

et al. 2020

Biology

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The use of cooled semen is relatively common in goats. There are a number of advantages of cooled semen doses, including easier handling of artificial insemination (AI) doses, transport, more AI doses per ejaculate, and higher fertility rates in comparison with frozen AI doses. However, cooled semen has a short shelf life. The objective of this study was to examine the effect of temperature and sperm concentration on the in vitro sperm quality during liquid storage for 48 h, including sperm motility and kinetics, response to oxidation, mitochondrial membrane potential (MMP) and DNA fragmentation in goats. Three experiments were performed. In the first, the effects of liquid preservation of semen at different temperatures (5 °C or 17 °C), durations (0, 24 and 48 h) and sperm concentrations (250 × 106 sperm/mL (1:2 dilution rate), 166.7 × 106 sperm/mL (1:3 dilution rate) or 50 × 106 sperm/mL (1:10 dilution rate)) on sperm motility and kinetics were studied. In the second experiment, the effect of temperature, sperm washing and concentration on sperm motility and DNA fragmentation was studied. Finally, the effect of sperm concentration and duration of storage at 5 °C on sperm motility, response to oxidative stress and MMP was examined. We found that refrigerated liquid storage of goat sperm impaired sperm quality, such as motility, MMP and response to oxidation, as storage time increased; however, sperm DNA fragmentation index was not significantly affected. Liquid storage at 5 °C preserved higher total motility than at 17 °C. Moreover, we observed that the reduction of sperm concentration below 500 × 106 sperm/mL did not seem to improve the quality of spermatozoa conserved in milk-based extender in the conditions tested.

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Section: Discussionmentioning

confidence: 98%

Section: Discussionmentioning

confidence: 99%

Effect of Sperm Concentration and Storage Temperature on Goat Spermatozoa during Liquid Storage

Sadeghi

Gallego

García-Colomer

et al. 2020

Biology

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“…Although cryopreservation can prolong the storage time of spermatozoa, the frozen-thawed spermatozoa possess the disadvantages of serious injuries in structure, poor fertilizing capacity and additional costs [4]. Liquid storage of spermatozoa at a reduced temperature is a practical alternative to cryopreservation when AI is performed within a relatively short time [3, 5]. Numerous studies are focusing on the preservation of spermatozoa utilizing liquid storage to improve the quality of spermatozoa in different species, such as the goat, boar and bull [2, 3, 6–8].…”

Section: Introductionmentioning

confidence: 99%

“…Numerous studies are focusing on the preservation of spermatozoa utilizing liquid storage to improve the quality of spermatozoa in different species, such as the goat, boar and bull [2, 3, 6–8]. However, a rapid decline in the viability and fertilizing potential of spermatozoa during liquid storage usually occurs with the increase in storage time [3, 5], limiting the widespread use of this technology.…”

Section: Introductionmentioning

confidence: 99%

Mechanisms of ROS-induced mitochondria-dependent apoptosis underlying liquid storage of goat spermatozoa

Liu

Han

Zhou

et al. 2019

Aging

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Liquid storage of spermatozoa is important for artificial insemination and herd genetic breeding. However, the extended time of storage inducing the rapid decline in spermatozoa quality limits the development of this technology. The molecular mechanisms underlying liquid storage of spermatozoa remain largely unexplored. In this study, the effects of liquid storage on functional quality of spermatozoa were assessed in goat (Capra hircus). The time-dependent decline in spermatozoa motility showed a strong correlation with the significant increase in apoptosis. Moreover, apoptosis-related ultrastructural changes were observed, especially the defects in mitochondria. A significant decrease in mitochondrial membrane potential and changes in the expression of mitochondrial apoptosis-related proteins indicated mitochondrial dysfunction and mitochondrial apoptotic pathway activation. Notably, the abnormally high level of reactive oxygen species (ROS) caused by liquid storage resulted in oxidative damage to mitochondria and accelerated mitochondria-dependent apoptosis, as demonstrated by the addition of ROS scavenger N-acetylcysteine. Furthermore, critical differentially expressed proteins involved in mitochondria-dependent apoptosis and antioxidant defense were identified and profiled by quantitative proteomic analysis, facilitating the understanding of molecular regulation of ROS-induced mitochondria-dependent apoptosis. These outcomes provide insights into the mechanisms underlying liquid storage of goat spermatozoa and enhance the progress of semen storage technology.

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“…Os principais fatores envolvidos nessa queda de fertilidade são as mudanças de temperatura durante o processamento, o estresse osmótico e tóxico representado pela exposição aos crioprotetores e a formação e dissolução de cristais de gelo extracelulares (Oliveira et al, 2013;Bertol et al, 2014;Quan et al, 2016;Losano, 2016). Assim a sobrevivência do sêmen criopreservado, depende de agentes que presentes na composição do meio diluidor, crioprotetores, tornando-se um fator crucial para a preservação da integridade celular e sucesso da fertilização após o congelamento (Leite et al, 2011).…”

Section: Introductionunclassified

Comparação de diluidores comerciais na motilidade, funcionalidade e integridade da membrana plasmática do espermatozoide bovino

Oliveira

Zandonaide

Severo³

et al. 2018

Revista Brasileira De Ciência Veterinária

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Effects of the Tris, Tes, or skim milk based extender on in vitro parameters of ram spermatozoa during liquid storage

Cited by 18 publications

References 29 publications

Effect of Sperm Concentration and Storage Temperature on Goat Spermatozoa during Liquid Storage

Effect of Sperm Concentration and Storage Temperature on Goat Spermatozoa during Liquid Storage

Mechanisms of ROS-induced mitochondria-dependent apoptosis underlying liquid storage of goat spermatozoa

Comparação de diluidores comerciais na motilidade, funcionalidade e integridade da membrana plasmática do espermatozoide bovino

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