Abstract. Formaldehyde exists in aquatic products, Lentinula edodes and other foods. For better application of blueberry leaf polyphenols as formaldehyde capture agent, the reaction conditions of blueberry leaf polyphenols with formaldehyde were studied under different polyphenols concentration, pH value, reaction temperature and reaction time. The effects of the pH, reaction temperature and reaction time on the reduction rate of formaldehyde were optimized by a response surface methodology (RSM). The results showed that pH most significantly affected on reactivity of blueberry leaf polyphenols with formaldehyde, followed by temperature and reaction time. The optimal polyphenols concentration was 0.2 %. The reactivity was the lowest at pH 3.0-4.0 and higher at strong acidic and alkaline conditions. Prolonging time facilitated the reaction under the higher temperature. There is a great prospect with blueberry leaf polyphenols as formaldehyde capture agent for aquatic products and other foods.