Effects of Thidiazuron on Cytokinin Autonomy and the Metabolism of N6-(Δ2-Isopentenyl)[8-14C]Adenosine in Callus Tissues of Phaseolus lunatus L.

Capelle, Susan C.; Mok, David W. S.; Kirchner, S.C.; Mok, Machteld C.

doi:10.1104/pp.73.3.796

Cited by 164 publications

(73 citation statements)

References 9 publications

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“…Such type of culture strategy employing different media for initiation and multiplication have been worked out successfully in many species (Faisal et al 2005;Ahmad and Anis 2007;Siddique and Anis 2007). Reportedly, TDZ exposed shoots/explants carry a high potential of multiplication on a basal media devoid of TDZ (Mok et al 1982;Capelle et al 1983). In contrast, the cultures grown continuously on TDZ containing media have fasciated and distorted shoots (Fig.…”

Section: Effect Of Thidiazuronmentioning

confidence: 99%

RETRACTED ARTICLE: Influencing micropropagation in Clitoria ternatea L. through the manipulation of TDZ levels and use of different explant types

Mukhtar

Ahmad²,

Khan³

et al. 2012

Physiol Mol Biol Plants

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A comparative performance of two explants types (CN and Nodal) for their efficiency to induce multiple shoot regeneration in Clitoria ternatea has been carried out. Thidiazuron (TDZ) in different concentrations (0.05-2.5 μM) was used as a supplement to the Murashige and Skoog's (MS) basal media. Explant type apart, two factors viz. concentration and exposure duration to TDZ played an important role in affecting multiple shoot regeneration. Cotyledonary node explants produced the best results at 0.1 μM TDZ, while in nodal explants the highest rate of shoot formation was achieved on MS medium supplemented with 1.0 μM TDZ. In both the explants, shoot multiplication increased when the regenerated shoots were subcultured on hormone free MS medium after 4 weeks of exposure to TDZ. Among the two, cotyledonary node explants produced considerably higher number of shoots at a comparatively lower concentration of TDZ than nodal explants. The regenerated shoots rooted best on MS medium containing 1.0 μM indole-3-butyric acid (IBA) and were successfully established in pots containing garden soil with 88 % survival rate. All the regenerated plants showed normal morphology and growth characteristics.

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Section: Effect Of Thidiazuronmentioning

confidence: 99%

RETRACTED ARTICLE: Influencing micropropagation in Clitoria ternatea L. through the manipulation of TDZ levels and use of different explant types

Mukhtar

Ahmad²,

Khan³

et al. 2012

Physiol Mol Biol Plants

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“…TDZ is not metabolized by the plants therefore its activity lasts longer than that of other cytokinins [8]. Moreover, it has been found that TDZ might promote the conversion of cytokinin ribonucleotides to more biologically active ribonucleosides [15]. These evidences might be the explanation of its effects on plants.…”

Section: Discussionmentioning

confidence: 95%

“…In fact, in the biosynthesis of chlorophyll, the last step is mediated by NADH protochlorophyllide reductase, which converts the protochlorophillide to chlorophyllide, and this process requires light [16]. Some previous studies have suggested that light regulates chlorophyll biosynthesis at transcriptional level while the cytokinins act at the post-trascriptional level [8,15,17]. The relationship between cytokinins and chlorophyll metabolism has also studied in light and dark grown wheat plants.…”

Section: Discussionmentioning

confidence: 99%

Effect of thidiazuron and gibberellic acid on leaf yellowing of cut stock flowers

Ferrante

Mensuali-Sodi²,

Serra³

2009

Open Life Sciences

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Plant hormones such as cytokinins and gibberellins are able to inhibit leaf yellowing in different species of cut flowers and potted plants.These hormones can be used alone or in combination among them for preserving chlorophyll in floriculture items. In the present study thidiazuron was tested alone or combined with GA 3 for delaying leaf yellowing of cut stock flowers during vase life. Cut flowers were placed in a controlled environment and treated for 24 hours with the following solutions: distilled water (control) or solutions containing 5, 10 µM thidiazuron (TDZ), 0.5 mM gibberellic acid (GA 3 ), or a combination of 0.5 mM GA 3 with 5 µM TDZ. The effect of treatments was evaluated by measuring chlorophyll content, ethylene production, leaf gas exchanges and chlorophyll a fluorescence. Results showed that TDZ was able to delay leaf yellowing in light during whole experimental period (30 days). The effect of TDZ on dark stored flowers was less effective, and also delayed chlorophyll losses for 10-12 days. TDZ and GA 3 combination did not show any synergistic nor beneficial effect. Gas exchange values such as net photosynthesis, vapour pressure deficit, stomatal conductance and water use efficiency were higher in the TDZ only treatment.

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“…Of the three cytokinins used, TDZ at 1.0 µM concentration was most effective (96%) for shoot proliferation ( Table 1). Several reports suggest that TDZ results in shoot regeneration better than other cytokinins [20][21][22][23]. According to Capelle et al [23], TDZ directly promotes growth due to its own biological activities similar to that of an N-substituted cytokinin or it may induce the synthesis and accumulation of an endogenous cytokinin.…”

Section: Resultsmentioning

confidence: 99%

TDZ-Induced High Frequency Plant Regeneration through Direct Shoot Organogenesis in Stevia rebaudiana Bertoni: An Important Medicinal Plant and a Natural Sweetener

Lata¹,

Chandra²,

Wang³

et al. 2013

AJPS

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An efficient high frequency plant regeneration protocol through direct organogenesis was developed for Sevia rebaudiana Bert. Nodal segments containing axillary buds were used as an explant and inoculated on Murashige and Skoog's (MS) medium containing 3% (w/v) sucrose, 0.8% (w/v) agar supplemented with various concentrations of benzyladenine (BA), kinetin (Kn) and thidiazuron (TDZ) ranging from 1.00 to 9.00 µM. Maximum multiple shoots (96%) were obtained in MS medium supplemented with 1.0 µM TDZ with an average of 60 shoots per culture, having an average shoot length of 6.0 cm. The best in vitro root induction (89%) was achieved on half strength MS medium without any growth regulator with an average of 24 roots per culture and root length of 7 cm. The rooted plantlets were successfully established in soil and grown to maturity at the survival rate of 95% in the indoor grow room. High-performance liquid chromatography was used to assess the stability in chemical profile and quantification of stevioside and rebaudioside A content of in vitro propagated S. rebaudiana plants and compared with their mother plant at the peak vegetative stage. Our results show no significant differences (p < 0.05) between the mother and in vitro propagated plants. Furthermore, fully developed in vitro propagated S. rebaudiana plants were also compared with mother plant for their gas and water vapour exchange characteristics and leaf anatomy. The results show that in vitro propagated and hardened plants of S. rebaudiana are morphologically as well as functionally comparable to each other and to their mother plant.

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Effects of Thidiazuron on Cytokinin Autonomy and the Metabolism of N⁶-(Δ²-Isopentenyl)[8-¹⁴C]Adenosine in Callus Tissues of Phaseolus lunatus L.

Cited by 164 publications

References 9 publications

RETRACTED ARTICLE: Influencing micropropagation in Clitoria ternatea L. through the manipulation of TDZ levels and use of different explant types

RETRACTED ARTICLE: Influencing micropropagation in Clitoria ternatea L. through the manipulation of TDZ levels and use of different explant types

Effect of thidiazuron and gibberellic acid on leaf yellowing of cut stock flowers

TDZ-Induced High Frequency Plant Regeneration through Direct Shoot Organogenesis in <i>Stevia rebaudiana</i> Bertoni: An Important Medicinal Plant and a Natural Sweetener

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Effects of Thidiazuron on Cytokinin Autonomy and the Metabolism of N6-(Δ2-Isopentenyl)[8-14C]Adenosine in Callus Tissues of Phaseolus lunatus L.

Cited by 164 publications

References 9 publications

RETRACTED ARTICLE: Influencing micropropagation in Clitoria ternatea L. through the manipulation of TDZ levels and use of different explant types

RETRACTED ARTICLE: Influencing micropropagation in Clitoria ternatea L. through the manipulation of TDZ levels and use of different explant types

Effect of thidiazuron and gibberellic acid on leaf yellowing of cut stock flowers

TDZ-Induced High Frequency Plant Regeneration through Direct Shoot Organogenesis in &lt;i&gt;Stevia rebaudiana&lt;/i&gt; Bertoni: An Important Medicinal Plant and a Natural Sweetener

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Effects of Thidiazuron on Cytokinin Autonomy and the Metabolism of N⁶-(Δ²-Isopentenyl)[8-¹⁴C]Adenosine in Callus Tissues of Phaseolus lunatus L.

TDZ-Induced High Frequency Plant Regeneration through Direct Shoot Organogenesis in <i>Stevia rebaudiana</i> Bertoni: An Important Medicinal Plant and a Natural Sweetener