Effects of three different PAHs on nitrogen-fixing bacterial diversity in mangrove sediment

Sun, Fu-Lin; Wang, You‐Shao; Sun, Cheng; Yang, Peng; Deng, Chao

doi:10.1007/s10646-012-0946-8

Cited by 44 publications

(18 citation statements)

References 46 publications

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“…However, laboratory isolates usually poorly represent the microbial populations living in the natural environment. Therefore, molecular techniques, such as denaturing gradient gel electrophoresis (DGGE) and sequencing of nifH gene clone libraries, have been applied to environmental samples to study the diversity of the diazotroph communities in the mangrove rhizospheres ( Flores-Mireles et al, 2007 ; Rameshkumar and Nair, 2009 ; Liu et al, 2012 ) and sediment samples ( Zhang et al, 2008 ; Dias et al, 2012 ; Romero et al, 2012 ; Sun et al, 2012 ). In recent years, pyrosequencing, which is a next generation sequencing approach that provides a more comprehensive perspective of the microbial community, has been employed to study the bacterial ( dos Santos et al, 2011 ) and diazotrophic community structures ( Gomes et al, 2010 ) in mangroves.…”

Section: Introductionmentioning

confidence: 99%

Anthropogenic impact on diazotrophic diversity in the mangrove rhizosphere revealed by nifH pyrosequencing

et al. 2015

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Diazotrophs in the mangrove rhizosphere play a major role in providing new nitrogen to the mangrove ecosystem and their composition and activity are strongly influenced by anthropogenic activity and ecological conditions. In this study, the diversity of the diazotroph communities in the rhizosphere sediment of five tropical mangrove sites with different levels of pollution along the north and south coastline of Singapore were studied by pyrosequencing of the nifH gene. Bioinformatics analysis revealed that in all the studied locations, the diazotroph communities comprised mainly of members of the diazotrophic cluster I and cluster III. The detected cluster III diazotrophs, which were composed entirely of sulfate-reducing bacteria, were more abundant in the less polluted locations. The metabolic capacities of these diazotrophs indicate the potential for bioremediation and resiliency of the ecosystem to anthropogenic impact. In heavily polluted locations, the diazotrophic community structures were markedly different and the diversity of species was significantly reduced when compared with those in a pristine location. This, together with the increased abundance of Marinobacterium, which is a bioindicator of pollution, suggests that anthropogenic activity has a negative impact on the genetic diversity of diazotrophs in the mangrove rhizosphere.

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Section: Introductionmentioning

confidence: 99%

Anthropogenic impact on diazotrophic diversity in the mangrove rhizosphere revealed by nifH pyrosequencing

et al. 2015

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“…On the other hand, PAHs in the soil exert pressure on various microbes which inhabit it. Microorganisms are sensitive to even low levels of pollutants in soil and rapidly respond to soil pollution, so microbial parameters are usually considered as potential bioindicators of soil pollution (Sun et al 2012).…”

Section: Introductionmentioning

confidence: 99%

Response of bacterial pdo1, nah, and C12O genes to aged soil PAH pollution in a coke factory area

Han

Liu

Zheng

et al. 2014

Environ Sci Pollut Res

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Soil pollution caused by polycyclic aromatic hydrocarbons (PAHs) is threatening human health and environmental safety. Investigating the relative prevalence of different PAH-degrading genes in PAH-polluted soils and searching for potential bioindicators reflecting the impact of PAH pollution on microbial communities are useful for microbial monitoring, risk evaluation, and potential bioremediation of soils polluted by PAHs. In this study, three functional genes, pdo1, nah, and C12O, which might be involved in the degradation of PAHs from a coke factory, were investigated by realtime quantitative PCR (qPCR) and clone library approaches. The results showed that the pdo1 and C12O genes were more abundant than the nah gene in the soils. There was a significantly positive relationship between the nah or pdo1 gene abundances and PAH content, while there was no correlation between C12O gene abundance and PAH content. Analyses of clone libraries showed that all the pdo1 sequences were grouped into Mycobacterium, while all the nah sequences were classified into three groups: Pseudomonas, Comamonas, and Polaromonas. These results indicated that the abundances of nah and pdo1 genes were positively influenced by levels of PAHs in soil and could be potential microbial indicators reflecting the impact of soil PAH pollution and that Mycobacteria were one of the most prevalent PAHs degraders in these PAH-polluted soils. Principal component analysis (PCA) and correlation analyses between microbial parameters and environmental factors revealed that total carbon (TC), total nitrogen (TN), and dissolved organic carbon (DOC) had positive effects on the abundances of all PAH-degrading genes. It suggests that increasing TC, TN, and DOC inputs could be a useful way to remediate PAH-polluted soils.

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“…La reducción de las bacterias en la rizósfera de L. hexandra en suelos con PF y PI, puede ser causado por la represión de la síntesis de proteína y DNA de los microorganismos, al estar expuesto a hidrocarburos policíclicos aromáti-cos como el naftaleno, fluoreno y pireno (Sun, Wang, Sun, Peng, & Deng, 2012;Penton et al, 2013). Además, el pH es un factor clave para el crecimiento de bacterias nitrificantes, ya que su óptimo crecimiento se encuentra en un pH neutral o ligeramente alcalino.…”

Section: Descontaminación De Htp (%)unclassified

Uso de Leersia hexandra (Poaceae) en la fitorremediación de suelos contaminados con petróleo fresco e intemperizado

Arias-Trinidad

Rivera-Cruz

Roldán-Garrigós

et al. 2016

RBT

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Use of Leersia hexandra (Poaceae) for soil phytoremediation in soils contaminated with fresh and weathered oil. The oil industry has generated chronic oil spills and their accumulation in wetlands of the state of Tabasco, in Southeastern Mexico. Waterlogging is a factor that limits the use of remediation technologies because of its high cost and low levels of oil degradation. However, Leersia hexandra is a grass that grows in these contaminated areas with weathered oil. The aim of the study was to evaluate the bacteria density, plant biomass production and phytoremediation of L. hexandra in contaminated soil. For this, two experiments in plastic tunnel were performed with fresh (E1) and weathered petroleum (E2) under waterlogging experimental conditions. The E1 was based on eight doses: 6 000, 10 000, 30 000, 60 000, 90 000, 120 000, 150 000 and 180 000 mg.kg -1 dry basis (d. b.) of total petroleum hydrocarbons fresh (TPH-F), and the E2, that evaluated five doses: 14 173, 28 400, 50 598, 75 492 and 112 142 mg. kg -1 d. b. of total petroleum hydrocarbons weathered (TPH-W); a control treatment with 2 607 mg.kg -1 d. b. was used. Each experiment, with eight replicates per treatment, evaluated after three and six months: a) microbial density of total free-living nitrogen-fixing bacteria (NFB) of Azospirillum (AZP) and Azotobacter group (AZT), for viable count in serial plate; b) dry matter production (DMP), quantified gravimetrically as dry weight of L. hexandra; and c) the decontamination percentage of hydrocarbons (PDH) by Soxhlet extraction. In soil with TPH-F, the NFB, AZP y AZT populations were stimulated five times more than the control both at the three and six months; however, concentrations of 150 000 and 180 000 mg.kg -1 d. b. inhibited the bacterial density between 70 and 89 %. Likewise, in soil with TPH-W, the FNB, AZP and AZT inhibitions were 90 %, with the exception of the 14 173 mg.kg -1 d. b. treatment, which stimulated the NFB and AZT in 2 and 0.10 times more than the control, respectively. The DMP was continued at the six months in the experiments, with values of 63 and 89 g in fresh and weathered petroleum, respectively; had no significant differences with the control (p≤0.05). The PDH reached values of 66 to 87 % both TPH-F and TPH-W at six months, respectively. These results demonstrated the ability the L. hexandra rhizosphere to stimulate the high NFB density, vegetal biomass production and phytoremediation of contaminated soils (with fresh and weathered petroleum), in a tropical waterlogging environment. Rev. Biol. Trop. 65 (1): 21-30. Epub 2017 March 01.

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Effects of three different PAHs on nitrogen-fixing bacterial diversity in mangrove sediment

Cited by 44 publications

References 46 publications

Anthropogenic impact on diazotrophic diversity in the mangrove rhizosphere revealed by nifH pyrosequencing

Anthropogenic impact on diazotrophic diversity in the mangrove rhizosphere revealed by nifH pyrosequencing

Response of bacterial pdo1, nah, and C12O genes to aged soil PAH pollution in a coke factory area

Uso de Leersia hexandra (Poaceae) en la fitorremediación de suelos contaminados con petróleo fresco e intemperizado

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