Effects of three male reproductive toxicants on rat cauda epididymal sperm motion

Toth, Gregory P.; Wang, S.-R.; McCarthy, H. W.; Tocco, Donald R.; Smith, Milton T.

doi:10.1016/0890-6238(92)90035-r

Cited by 42 publications

(11 citation statements)

References 20 publications

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“…Sperm were removed from the right cauda epididymis and suspended in bovine serum albumin and maintained at 37°C. Sperm were then analyzed microscopically using a videomicrography system (Cell Soft Automated Semen Analyzer, Cryo Resources, Ltd., Montgomery, New York) (Toth et al, 1992). Bouin's fixative was used to fix the testes and epididymides.…”

Section: Opto-varimex Open-field Activity Evaluation Tests (Columbus mentioning

confidence: 99%

Reproductive Toxicity Screen of 1,3,5-Trinitrobenzene Administered in the Diet of Sprague-Dawley Rats

Kinkead¹,

Wolfe²,

Flemming³

et al. 1995

Toxicol Ind Health

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Several Army installations targeted for restoration have measurable quantities of 1,3,5-trinitrobenzene (TNB) in the soil and groundwater. As part of the process of developing environmental and health effects criteria for restoration, a modified Screening Information Data Set (SIDS) reproductive study was performed. Male and female Sprague-Dawley rats received a diet containing approximately 30, 150, or 300 mg TNB/kg diet. Mating occurred following 14 days of treatment. All dams, one-half the males, and representative pups were maintained for a total of 90 days of treatment. No mortality occurred during the study; however, a decrease in mean body weights was noted in both sexes of high-dose rats. A dose-related effect was noted in measurements of sperm function/activity. Sperm depletion and degeneration of the seminiferous tubules were noted histopathologically. Methemoglobinemia and splenic hemosiderosis were common findings in the high- and mid-dose levels of both sexes at necropsy. No adverse effects were noted in mating or fertility indices. No significant treatment-related differences were found in length of gestation, sex ratio, gestation index, or mean number of pups per litter.

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Section: Opto-varimex Open-field Activity Evaluation Tests (Columbus mentioning

confidence: 99%

Reproductive Toxicity Screen of 1,3,5-Trinitrobenzene Administered in the Diet of Sprague-Dawley Rats

Kinkead¹,

Wolfe²,

Flemming³

et al. 1995

Toxicol Ind Health

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“…These compounds are converted to the common metabolite (S)-3-chlorolactaldehyde, a competitive substrate inhibitor of the GAPD isoform in sperm that reduces fertility in several mammalian species [6,[26][27][28][29][30][31]. Infertility mediated by (S)-3-chlorolactaldehyde is associated with reduced motility and reduced enzymatic activity in sperm, at concentrations that do not inhibit activity of the GAPD isoform in somatic tissues [32].…”

Section: Introductionmentioning

confidence: 98%

Glyceraldehyde 3-phosphate dehydrogenase-S protein distribution during mouse spermatogenesis

Bunch

1998

Biology of Reproduction

145

126

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The spermatogenic cell-specific isoform of glyceraldehyde 3-phosphate dehydrogenase (GAPD-S) may regulate glycolysis and energy production required for sperm motility. Although the steady-state level of Gapd-s mRNA is maximal at step 9 of mouse spermatogenesis, GAPD-S protein was not detected by immunohistochemistry until steps 12-13. This result suggests that Gapd-s is translationally regulated. Western blot analysis of isolated germ cells confirmed that GAPD-S is not detected in pachytene spermatocytes or round spermatids. A major immunoreactive protein migrating with a molecular weight (M(r)) of 69,200 was observed in condensing spermatids and cauda sperm. Additional minor proteins that migrated at M(r) 55,200, 32,500, and 27,500 were detected in sperm. The molecular weight of GAPD-S is higher than the predicted molecular weight of 47,445, apparently due to a proline-rich 105-amino acid domain at the N-terminus. Recombinant GAPD-S protein lacking the proline-rich region migrated at M(r) 38,250, comparably to somatic GAPD, which also lacks the proline-rich domain. Indirect immunofluorescence demonstrated that GAPD-S is restricted to the principal piece in the sperm flagellum. Western blot analysis indicated that GAPD-S is tightly associated with the fibrous sheath of the flagellum, consistent with a potential role in regulating sperm motility.

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“…In this study, several sperm motility parameters reduced significantly in all treated groups. Toth et al (1992) reported that sperm motion parameters were altered obviously by some toxicants at the dose not affecting other reproductive endpoints. El-Sabeawy et al (1998) showed that some toxicants may have special effect on sperm motility.…”

Section: Discussionmentioning

confidence: 98%

Effect of oral administration of terephthalic acid on testicular functions of rats

Cui

Dai

et al. 2004

Toxicology

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Effects of three male reproductive toxicants on rat cauda epididymal sperm motion

Cited by 42 publications

References 20 publications

Reproductive Toxicity Screen of 1,3,5-Trinitrobenzene Administered in the Diet of Sprague-Dawley Rats

Reproductive Toxicity Screen of 1,3,5-Trinitrobenzene Administered in the Diet of Sprague-Dawley Rats

Glyceraldehyde 3-phosphate dehydrogenase-S protein distribution during mouse spermatogenesis

Effect of oral administration of terephthalic acid on testicular functions of rats

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