Effects of three virus diseases on three pear cultivars

Lemoine, J.; Michelesi, Jean-Claude

doi:10.1016/0304-4238(90)90018-a

Cited by 7 publications

(1 citation statement)

References 4 publications

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“…In 2018, apple rubbery wood viruses 1 and 2 (ARWV1 and 2; genus Rubodvirus ) were identified in apple trees showing rubbery wood symptoms in Canada; later, ARWV2 was found in Chinese pear samples (Rott et al, 2018; Wang et al, 2019). The disease associated with these two nsRNA viruses (i.e., apple rubbery wood disease) can reduce fruit yield by up to 30% in apple and 50% in pear (Lemoine & Michelesi, 1990). Wright et al (2018) identified CCGaV, ARWV1 and 2 infecting apples in Washington, however, the incidence of nsRNA viruses in California orchards has not been investigated.…”

Section: Introductionmentioning

confidence: 99%

Incidence and detection of negative‐stranded RNA viruses infecting apple and pear trees in California

Diaz‐Lara

Wunderlich

Nouri³

et al. 2021

Journal of Phytopathology

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Novel negative‐stranded RNA (nsRNA) viruses have been recently identified in multiple agronomic crops, including pome fruit trees. Citrus concave gum‐associated virus (CCGaV), citrus virus A (CiVA) and apple rubbery wood viruses 1 and 2 (ARWV1 and 2) are examples of such viruses. Given the novelty and lack of information about these pathogens in Californian orchards, in this study, real‐time RT‐PCR assays for CCGaV, CiVA, ARWV1 and 2 were developed and employed in a field survey. Initially, the new assays were challenged against a comprehensive set of positive and negative samples, previously analysed by high‐throughput sequencing (HTS), to determine specificity. Aiming to investigate the presence of nsRNA viruses in California apple and pear orchards, 186 samples were collected from 21 different locations. As a result, 79 (42%) samples were found to be infected by these viruses in single or mixed infections. The incidence of each virus in relation to the total number of samples was 36%, 15%, 11% and 0% for ARWV2, CCGaV, ARWV1 and CiVA respectively. Overall, not considering the no detected CiVA, the other three nsRNA viruses were widely distributed among sampled orchards. To further validate the reliability of the new real‐time RT‐PCR assays, six samples tested positive during the survey were screened by previously described detection assays and HTS. This is the first detection of these nsRNA viruses in California, which may represent an issue in apple and pear production.

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