Effects of Thymoquinone on Oxidative Stress in the Testicular Tissue of Reserpinized Rats

Abstract

Objective: The objective of the study was to research the oxidant and antioxidant activity of Thymoquinone (TQ) in the testicular tissue of Reserpinized rats. Methods: Eighteen rats were divided into three groups and each group had six animals: 1) Control (C) group: Received ip. 1% Tween 80; 2) Reserpine (R) group: Received Reserpine; 3) Reserpine + TQ (R+T) group: Received Reserpine and TQ. Reserpine was injected intraperitoneally 0.2 mg/kg and TQ was administered intragastrically 10 mg/kg once daily. The rats were treated for 14 consecutive days. At the end of the study, total nitric oxide (NOx) levels, malondialdehyde (MDA) levels and glutathione (GSH) levels in the testicular tissue were examined. Results: A statistically significant increase was observed in the NOx levels (p=0.001) and MDA levels of testicular tissue in the R group (p<0.001). A decrease was observed in the GSH levels (p<0.001). It was found out that there was a decrease in NOx and MDA levels while there was an increase in GSH levels in the R+T group (p<0.001). Conclusion: Reserpine caused an increase in oxidative stress and a decrease in antioxidant capacity in testicular tissue. TQ yielded recovery of Reserpine induced toxicity by decreasing oxidative stress markers and increasing antioxidant capacity.

Bir Flavonoid Olan Gossypin'in, Standard Bakteri ve Maya Suşları Üzerindeki Antibakteriyel, Antifungal, Antibiyofilm, Antiadherent ve Antiinvazif Aktivitelerinin İn vitro Olarak Araştırılması

“…Epigallocatechin gallate (EGCG), curcumin, resveratrol, Nigella sativa, apigenin ve benzer birçok doğal ajan ile ilgili binlerce bilimsel çalışma görmek mümkündür [14][15][16][17][18][19][20][21] . Ancak gossypin'in tarihi diğer doğal ajanlar kadar eski olmadığından dolayı, hakkında bu kadar detaylı bilgi edinmek mümkün olmamıştır.…”

Occurrence and Bioactivity Diversity of Thymoquinone: An Overview

“…TQ ameliorated testicular damage and improved sperm quality in varicocele-induced adolescent rats by reducing apoptosis, oxidative stress, and lipid peroxidation [158]. TQ reduces oxidative stress in the testicular tissue of reserpinized rats by decreasing the oxidative stress and increasing the decreased antioxidant capacity [159]. TQ supplementation limited bacterial prostatitis because of a substantial boost in the antioxidant enzymes [160].…”