Effects of titanium surface anodization with CaP incorporation on human osteoblastic response

Oliveira, Natássia Cristina Martins; Moura, Camilla Christian Gomes; Zanetta‐Barbosa, Darceny; Mendonça, Daniela Baccelli Silveira; Cooper, Lyndon F.; Mendonça, Gustavo; Dechichi, Paula

doi:10.1016/j.msec.2013.01.002

Cited by 27 publications

(17 citation statements)

References 31 publications

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“…At day 21 in this study there was only modest upregulations of ×1.7 and ×1.4‐fold changes for the cpTi and the CaP‐TiTi surfaces, respectively. Previous studies investigating RUNX2 expression have included an earlier time point, that is, at days 1, 3, or 4 . −38 The inclusion of earlier time points in the present study may have provided a better baseline and more meaningful comparison of fold change analysis for RUNX2.…”

Section: Discussionmentioning

confidence: 95%

Osteoblastic differentiation of periodontal ligament stem cells on non‐stoichiometric calcium phosphate and titanium surfaces

Winning

Robinson

Boyd

et al. 2017

J Biomedical Materials Res

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Bioactive materials offer particular clinical benefits in the field of dental implantology, where differentiation of stem cells towards an osteoblastic lineage is required for osseointegration and appropriate function of implants in vivo. The aim of this study was to evaluate the osteoblastic response of Stro‐1 +ve periodontal ligament stem cells (PDLSCs) to three well‐characterized biomaterial surfaces: an abraded titanium surface (cpTi) control; a polycrystalline titanium surface, with both micro and nanotopography produced by radio frequency magnetron sputtering (TiTi); and the same surface incorporating a sputter deposited calcium phosphate coating (CaP‐TiTi). The CaP‐TiTi surfaces were nonstoichiometric, carbonated, and calcium rich with a Ca/P ratio of 1.74. PDLSCs were grown on each surface in the absence of supplementary osteogneic‐inducing agents. Osteoblastic responses were assessed for up to 21 days in culture by measuring gene expression using real time q‐PCR and via assessment of intracellular alkaline phosphatase (ALP) activity. Gene expression analysis for the CaP‐TiTi surfaces showed a significant late stage up‐regulation of Secreted Phosphoprotein 1. Additionally, there was a significant up‐regulation of the Wnt signaling genes β‐catenin and Wnt Family Member 5 A on days 14 and 21, respectively for the CaP‐TiTi surface. A significant increase in intracellular ALP at day 21 for the CaP‐TiTi surface was also observed. These data suggest that the CaP‐TiTi surfaces provide the bioactive conditions required for direct osteoblastic differentiation of PDLSCs. © 2017 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 105A: 1692–1702, 2017.

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Section: Discussionmentioning

confidence: 95%

Osteoblastic differentiation of periodontal ligament stem cells on non‐stoichiometric calcium phosphate and titanium surfaces

Winning

Robinson

Boyd

et al. 2017

J Biomedical Materials Res

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“…More CaP deposition is believed to promote osteoblast cell growth and attachment on titanium [28][29][30] because CaP is necessary component composing osteoblast cells. Therefore the increased osteoblast cell attachment on ECAPed CP-Ti can be understood by this mechanism.…”

Section: Discussionmentioning

confidence: 99%

Enhanced Surface Precipitates on Ultrafine-Grained Titanium in Physiological Solution

Zhou

Wang

Zou

2017

Metals

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Enhanced cell adherence to the surface of nanocrystallized commercially pure titanium (CP-Ti) was observed by several authors. However, the understanding of the surface modification of Ti in a physiological solution due to nanocrystallized grain size has not been reached. In this work, equal channel angular pressing (ECAP) was applied to manufacturing ultrafine grained CP-Ti. Martensite and Widmanstatten microstructures were also obtained for comparison. The CP-Ti pieces with different microstructures were subjected to soaking tests in a simulated body fluid. Electrochemical impedance spectroscopy (EIS) measurements, X-ray photoelectron spectroscopy (XPS) and scanning electron microscope (SEM), energy dispersive spectrometer (EDS) were used to characterize the surfaces. The results show the surface precipitates mainly contain Ti, O, Ca and P. The quantity of precipitates on ECAPed CP-Ti is the largest among different specimens corresponded to the observation of the thickest layer formation on ECAPed CP-Ti found by EIS. EDS results show more CaPO and less Ti are included on ECAPed Ti comparing to the deposits on other two types of specimens. Smaller numbers of precipitates and denser film are produced on the surface of the water-quenched CP-Ti. The regeneration kinetics of the CaP precipitates evaluated by Gibbs free energy is introduced to interpret the precipitating behaviors on different CP-Ti specimens.

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“…Moreover, it would appear that only two studies found in the literature specifically analyze BMP-2 production by osteoblasts in vitro , although the use of exogenous BMP-2 in in vitro studies on titanium discs is frequently mentioned in the literature ( 30 , 31 ).…”

Section: Discussionmentioning

confidence: 99%

Production of bone mineral material and BMP-2 in osteoblasts cultured on double acid-etched titanium

Velázquez-Cayón

Castillo-Dalí²,

Corcuera-Flores³

et al. 2017

Med Oral

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BackgroundThe study of osteoblasts and their osteogenic functions is essential in order to understand them and their applications in implantology. In this sense, this study try to study BMP-2 production and bone matrix deposition, in addition to other biological variables, in osteoblasts cultured on a rough double acid-etched titanium surface (Osseotite®, Biomet 3i, Palm Beach Garden, Florida, USA) in comparison to a smooth titanium surface (machined) and a control Petri dish.Material and MethodsAn in vitro prospective study. NHOst human osteoblasts from the femur were cultured on three different surfaces: Control group: 25-mm methacrylate dish (n = 6); Machined group: titanium discs with machined surface (n = 6) and Experimental group: titanium discs with a double acid-etched nitric and hydrofluoric Osseotite® acid surface (n = 6). A quantification of the mitochondrial membrane potential, and studies of apoptosis, mobility and adhesion, bone productivity (BMP-2) and cellular bone synthesis were carried out after culturing the three groups for forty-eight hours.ResultsA statistically significant difference was observed in the production of BMP-2 between the experimental group and the other two groups (22.33% ± 11.06 vs. 13.10% ± 5.51 in the machined group and 3.88% ± 3.43 in the control group). Differences in cellular bone synthesis were also observed between the groups (28.34% ± 14.4% in the experimental group vs. 20.03% ± 6.79 in the machined group and 19.34% ± 15.93% in the control group).ConclusionsIn comparison with machined surfaces, Osseotite® surfaces favor BMP-2 production and bone synthesis as a result of the osteoblasts in contact with it. Key words:BMP-2, Cytoskeleton, cell culture, bone matrix, apoptosis, cell viability.

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Effects of titanium surface anodization with CaP incorporation on human osteoblastic response

Cited by 27 publications

References 31 publications

Osteoblastic differentiation of periodontal ligament stem cells on non‐stoichiometric calcium phosphate and titanium surfaces

Osteoblastic differentiation of periodontal ligament stem cells on non‐stoichiometric calcium phosphate and titanium surfaces

Enhanced Surface Precipitates on Ultrafine-Grained Titanium in Physiological Solution

Production of bone mineral material and BMP-2 in osteoblasts cultured on double acid-etched titanium

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