Effects of topography and composition of titanium surface oxides on osteoblast responses

Zhu, Xiaolong; Chen, Jun; Scheideler, Lutz; Reichl, R.; Geis-Gerstorfer, J

doi:10.1016/j.biomaterials.2003.11.011

Cited by 484 publications

(343 citation statements)

References 55 publications

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“…However, despite their notable advantages, the bioinert nature of these materials could be involved in the production of nonadherent fibrous tissue at the bone-material biointerface,affecting in some cases the implant stability and increasing the risk of the implant loosening over time [11]. Several studies have shown that surface properties such as porosity, roughness, hydrophobic/hydrophilic characteristics, texture, morphology, composition and free surface energy are important factors to stimulate cells adhesion, morphologicalchanges, and enhancing cells proliferation and differentiation around the implant as well [12][13][14].…”

Section: Introductionmentioning

confidence: 99%

“…This behavior is caused by the incorporation of phosphorus into the oxide layers from electrolyte [27][28][29]. After this process, in vivo and in vitro assays have allowed some researchers to report improvements in implants of Ti, biocompatibility associated to surfaces that were previously treated by different techniques; in these studies, micro-roughness and incorporation of species within the anodic layers were responsible of higher biocompatibility [10,12,30,31].…”

Section: Introductionmentioning

confidence: 99%

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Osseointegration improvement by plasma electrolytic oxidation of modified titanium alloys surfaces

Echeverry‐Rendón

Galvis

Giraldo

et al. 2015

J Mater Sci: Mater Med

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Titanium (Ti) is a material frequently used in orthopedic applications, due to its good mechanical properties and high corrosion resistance. However, formation of a non-adherent fibrous tissue between material and bone drastically could affect the osseointegration process and, therefore, the mechanical stability of the implant. Modifications of topography and configuration of the tissue/ material interface is one of the mechanisms to improve that process by manipulating parameters such as morphology and roughness. There are different techniques that can be used to modify the titanium surface; plasma electrolytic oxidation (PEO) is one of those alternatives, which consists of obtaining porous anodic coatings by controlling parameters such as voltage, current, anodizing solution and time of the reaction. From all of the above factors, and based on previous studies that demonstrated that bone cells sense substrates features to grow new tissue, in this work commercially pure Ti (c.p Ti) and Ti6Al4V alloy samples were modified at their surface by PEO in different anodizing solutions composed of H2SO4 and H3PO4 mixtures. Treated surfaces were characterized and used as platforms to grow osteoblasts; subsequently, cell behavior parameters like adhesion, proliferation and differentiation were also studied. Although the results showed no significant differences in proliferation, differentiation and cell biological activity, overall results showed an important influence of topography of the modified surfaces compared with polished untreated surfaces. Finally, this study offers an alternative protocol to modify surfaces of Ti and their alloys in a controlled and reproducible way in which biocompatibility of the material is not compromised and osseointegration would be improved.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Osseointegration improvement by plasma electrolytic oxidation of modified titanium alloys surfaces

Echeverry‐Rendón

Galvis

Giraldo

et al. 2015

J Mater Sci: Mater Med

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“…Many surface properties have been demonstrated to influence the efficiency of cell attachment and proliferation as well as the phenotype 1,2 . Some of these properties include topography 3,4 , elasticity 5 , surface chemistry 6 , hydrophobicity 7,8 and ligand density 9 . For this reason, much attention has been directed towards the surface modification of substrates, for example to enhance or minimise cellular attachment 10,11 .…”

Section: Introductionmentioning

confidence: 99%

“…Thus, with the use of gradients, it is possible to effectively reduce sample size and analysis time in in vitro experiments. For example, recent studies 3,4 have shown that surface topography gradient surfaces can be used to screen for specific cellular morphology. Cell attachment was significantly reduced within a certain range of topography, while other topography regions stimulate the cells to assume specific morphologies.…”

Section: Introductionmentioning

confidence: 99%

Preparation of two-directional gradient surfaces for the analysis of cell-surface interactions

Clements

Khung

Thissen

et al. 2007

BioMEMS and Nanotechnology III

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The ability to evaluate and control the cellular response to substrate materials is the key to a wide range of biomedical applications ranging from diagnostic tools to regenerative medicine. Gradient surfaces provide a simple and fast method for investigating optimal surface conditions for cellular responses such as attachment and growth. By using two orthogonal gradients on the same substrate, a large space of possible combinations can be screened simultaneously. Here, we have investigated the combination of a porous silicon (pSi) based topography gradient with a plasma polymer based thickness gradient. pSi was laterally anodised on a 1.5 x 2.5cm 2 silicon surface using hydrofluoric acid to form a pore size gradient along a single direction. The resulting pSi was characterised by SEM and AFM and pore sizes ranging from macro to mesoporous were found along the surface. Plasma polymerisation was used to form a thickness gradient orthogonal to the porous silicon gradient. Here, allylamine was chosen as the monomer and a mask placed over the substrate was used to achieve the thickness gradient. The analysis of this chemistry based gradient was carried out using profilometry and XPS. It is expected that orthogonal gradient substrates will be used increasingly for the in vitro screening of materials used in biomedical applications.

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“…According to Zhu et al 10 modified surfaces improve biocompatibility, increase cellular adhesion and proliferation. Studies by Rocci et al 5 and Bahat 11 have indicated that modifications of the surface and macro design of the implant are particularly important for its longevity.…”

Section: IImentioning

confidence: 99%

The effects of subcutaneous injection of nicotine on osseointegration of machined and anodized implants in rabbits

Linden

Bittencourt²,

Carli³

et al. 2018

Acta Cir. Bras.

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Purpose: To evaluate the influence of subcutaneous injection nicotine in osseointegration process on different implant surfaces. Methods: Twenty-two male rabbits were distributed into two groups according to the subcutaneous injections: (1) nicotine 3 mg/day/kg and (2) 0.9 % NaCI 3 mL/day/kg, three times a day; subgroups were then designated-machined and anodized implants were placed in the right and left tibia bones, respectively. The animals were submitted euthanasia after periods of eight weeks to determine nicotine and cotinine levels, alkaline phosphatase and biomechanical analysis. Results: The plasmatic levels of nicotine and cotinine were 0.5 ± 0.28 ng/mL and 9.5 ± 6.51 ng/mL, respectively. The alkaline phosphatase analyses in blood levels in control group were observed 40.8 ± 11.88 UI/L and 40.75 ± 12.46 UI/L, for the surfaces machined and anodized, respectively. In the test group was observed levels 37.9 ± 4.84 UI/L, for both implant surfaces. No significant differences were observed between control and test groups and between the implant surfaces regarding alkaline phosphatase blood levels. For biomechanics, no significant differences were observed in control group between the machined (25±8.46 Ncm) or anodized (31.2 ± 6.76 Ncm) implants. However, the treatment with nicotine induced higher torque than control in both machined (38.3 ± 13.52 Ncm) and anodized (35.5 ± 14.17 Ncm) implants, with p = 0.0024 and p = 0.0121, respectively. Conclusion: Subcutaneous injection of nicotine following implant insertion didn't have effect on osseointegration, independently from the implant surface.

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Effects of topography and composition of titanium surface oxides on osteoblast responses

Cited by 484 publications

References 55 publications

Osseointegration improvement by plasma electrolytic oxidation of modified titanium alloys surfaces

Osseointegration improvement by plasma electrolytic oxidation of modified titanium alloys surfaces

Preparation of two-directional gradient surfaces for the analysis of cell-surface interactions

The effects of subcutaneous injection of nicotine on osseointegration of machined and anodized implants in rabbits

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