Effects of total flavonoids from Drynariae Rhizoma prevent bone loss in vivo and in vitro

et al. 2020

Preprint

Background: Caged layer osteoporosis (CLO) is a common bone metabolic disease and is threatening the modern poultry industry. The objective of this study was to evaluate effects of total flavonoids of Rhizoma Drynariae (TFRD), a Chinese herbal extract, on bone health, egg quality and production performance in caged laying hens. Totals of 144 Lohmann pink-shell laying hens of 32-wk-old were randomly allocated to 4 groups. The control (CON) group was fed a basal diet, the low calcium diet (LCD) group was fed a low calcium diet, and the TFRD1 and TFRD2 groups were fed LCD supplemented with 0.5 and 2.0 g/kg TFRD, respectively. The experiment lasted 9 wks. Results: Supplying 2.0 g/kg TFRD produced protective effects on calcium deficiency-induced bone loss in caged laying hens, including enhanced femur bone mineral density (P < 0.05), improved bone microarchitecture deterioration, reduced serum levels of alkaline phosphatase and tartrate resistant acid phosphatase activities (both P < 0.05) and osteocalcin content (P < 0.05), down-regulated mRNA expressions of receptor activator of nuclear factor kappa-B ligand (RANKL) (P < 0.01) and up-regulated runt related transcription factor 2 (RUNX2) and osteoprotegerin (OPG) levels (both P < 0.01). Furthermore, compared to LCD group, 2.0 g/kg TFRD treatment had lower malondialdehyd levels (P < 0.01), and higher total antioxidant capacity (P < 0.05) and glutathione peroxidase (P < 0.05) activities, which contributed to the protective effects of bone loss. TFRD supplementation did not affect egg quality while 2.0 g/kg TFRD treatment improved laying rate (P < 0.05) and decreased feed conversion ratio (P < 0.05). Conclusions: These findings suggested that TFRD has beneficial effects on bone health and production performance in caged laying hens, which can be used for the prevention of CLO.

Section: Discussionmentioning

confidence: 95%

Total flavonoids of Chinese herb Rhizoma Drynariae alleviates bone loss in caged laying hens

et al. 2020

Preprint

Section: Discussionmentioning

confidence: 95%

Total flavonoids of Chinese herb Rhizoma Drynariae alleviates bone loss in caged laying hens

et al. 2019

Preprint

Background: Caged layer osteoporosis (CLO) is a common bone metabolic disease and is threatening the modern poultry industry. The objective of this study was to evaluate effects of total flavonoids of Rhizoma Drynariae (TFRD), a Chinese herbal extract, on bone health, egg quality and production performance in caged laying hens. Totals of 144 Lohmann pink-shell laying hens of 32-wk-old were randomly allocated to 4 groups (CON, LCD, TFRD1 and TFRD2, respectively). The CON group was fed a basal diet, the LCD group was fed a low calcium diet (LCD) and the TFRD1 and TFRD2 groups were fed LCD supplemented with 0.5 and 2.0 g/kg TFRD, respectively.Results: Supplying 2.0 g/kg TFRD produced protective effects on calcium deficiency-induced bone loss in caged laying hens, including reduced serum levels of alkaline phosphatase and tartrate resistant acid phosphatase activities (both P < 0.05) and osteocalcin content (P < 0.05), enhanced femur bone mineral density (P < 0.05), improved bone microarchitecture deterioration, down-regulated mRNA expressions of RANKL (P < 0.01) and up-regulated RUNX2 and OPG levels (both P < 0.01). Furthermore, compared to LCD group, 2.0 g/kg TFRD treatment had lower malondialdehyd levels (P < 0.01), and higher total antioxidant capacity (P < 0.05) and glutathione peroxidase (P < 0.05) activities, which contributed to the protective effects of bone loss. TFRD supplementation did not affect egg quality while 2.0 g/kg TFRD treatment improved laying rate (P < 0.05) and decreased feed conversion ratio (P < 0.05).Conclusions: These findings suggested that TFRD has beneficial effects on bone health and production performance in caged laying hens, which can be used for the prevention of CLO.

“…Traditionally, TFRD have long been considered an ideal drug for the treatment of bone-related disease ( Song et al., 2016 ). Osteoporosis in caged laying hens is a common bone metabolic disease which leads to bone fragility, and the incidence rates of fracture up to 30% during the laying period ( Whitehead and Fleming, 2000 ; Johnsson et al., 2015 ).…”

Section: Discussionmentioning

confidence: 99%

“…Total flavonoids from Rhizoma Drynariae is widely used for the treatment of bone fractures or related diseases in Asian countries. It has been proven that TFRD has antiosteoporosis activity by inhibiting the bone resorption and stimulating bone formation ( Wang et al., 2011 ) and finally achieves the goal of prevention and treatment of osteoporosis in humans and rat model ( Song et al., 2016 ). Recently, studies found that TFRD has therapeutic effect on tibial dyschondroplasia in broilers ( Yao et al., 2018 ).…”

Section: Introductionmentioning

confidence: 99%

Dietary supplementation of total flavonoids from Rhizoma Drynariae improves bone health in older caged laying hens

et al. 2020

Poultry Science

Caged layer osteoporosis ( CLO ) is a common bone metabolism diseases and poses a great threat to the production of laying hens. So far, there is no effective nutrition intervention to prevent CLO. The objective of this study was to evaluate the effects of dietary total flavonoids from Rhizoma Drynariae ( TFRD ), a Chinese herbal, on bone health, egg quality, and serum antioxidant capacity of caged laying hens. A total of two hundred sixteen, 54-wk-old Lohmann Pink-shell laying hens at were allocated to 3 groups with 6 replicates of 12 hens per replicate. The control group was fed a basal diet ( BD ) and 2 treatment groups additionally supplied with 0.5 or 2.0 g/kg TFRD, respectively. Results showed that supplying 2.0 g/kg TFRD enhanced the activities of serum total antioxidant capacity ( P < 0.01) and glutathione peroxidase ( P < 0.05) and had higher femur and tibia bone mineral density (both P < 0.05) compared with the control group. Dietary 2.0 g/kg TFRD also reduced the activities of serum alkaline phosphatase ( P < 0.01), tartrate resistant acid phosphatase ( P < 0.01), and the contents of osteocalcin ( P < 0.01). Furthermore, tibia histomorphology observation showed that the microstructure of bone tissue was improved after TFRD treatment. Egg quality was not affected by TFRD while the egg weight significantly increased ( P < 0.01). These findings suggested that TFRD has beneficial effects on bone health in older caged laying hens.