Effects of transforming growth factors-alpha and -beta on proliferation and apoptosis of rat theca-interstitial cells

Pehlivan, Tugce; Mansour, Ali; Spaczyński, Robert; Duleba, A.P.

doi:10.1677/joe.0.1700639

Cited by 21 publications

(11 citation statements)

References 53 publications

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“…This is consistent with a previous report showing that TGFβ does not induce apoptosis in a cell line of normal ovarian epithelial cells, even though it does inhibit ( 3 H) thymidine uptake in the cells (Havrilesky et al, 1995). It is also consistent with the finding that TGFβ by itself has no effect on apoptosis in rat thecal interstitial cells (Pehlivan et al, 2001). Further, it is consistent with a recent report that Smad 3 is functional in some ovarian cancers (Dunfield et al, 2002).…”

Section: Discussionsupporting

confidence: 92%

Smad 3 regulates proliferation of the mouse ovarian surface epithelium

et al. 2003

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Smad 3 is a signaling intermediate for the transforming growth factor beta (TGF␤) family; however, little is known about the role this protein plays in the regulation of the ovarian surface epithelium (OSE). Using a transgenic mouse model, we found that in the absence of Smad 3 there was a distinct morphological alteration of OSE cells. Wild-type (WT) OSE was flat with thin cells, while Smad 3-deficient (Smad 3 -/-) OSE was thick with plump cuboidal cells. WT OSE had less immunostaining for proliferating cell nuclear antigen (PCNA) and estrogen receptor alpha (ER␣) than Smad 3 -/-OSE. However, there were no differences in the number of apoptotic cells or Bax and Bcl-2 levels between WT and Smad 3 -/-OSE. Although WT mice had higher levels of serum estradiol than Smad 3 -/-mice, WT and Smad 3 -/-mice had similar levels of progesterone. These data suggest that Smad 3 regulates OSE morphological appearance and proliferation in the absence of high serum estradiol levels or alterations in progesterone levels. Anat Rec Part A 273A: 681-686, 2003.

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Section: Discussionsupporting

confidence: 92%

Smad 3 regulates proliferation of the mouse ovarian surface epithelium

et al. 2003

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“…For example, members of the transforming growth factor-β (TGF-β) family are expressed by oocytes, granulosa cells, and theca cells in a developmental-stage dependent manner and play roles in proliferation/atresia of granulosa/theca cells, steroidogenesis, oocyte maturation, ovulation, and luteinization (Pehlivan et al, 2001; Juengel et al, 2005; Knight et al, 2006). The members of downstream signaling molecules (Smads) and binding proteins (follistatin) of TGF-β family are also expressed in the ovary, and function in follicular development and luteinization (Drummond et al, 2002; Xu et al, 2002).…”

Section: Introductionmentioning

confidence: 99%

Induction of Ski Protein Expression upon Luteinization in Rat Granulosa Cells

Kim¹,

Kim

Park

et al. 2012

Asian Australas. J. Anim. Sci

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Ski protein is implicated in proliferation/differentiation in a variety of cells. We had previously reported that Ski protein is present in granulosa cells of atretic follicles, but not in preovulatory follicles, suggesting that Ski has a role in apoptosis of granulosa cells. The alternative fate of granulosa cells other than apoptosis is to differentiate to luteal cells; however, it is unknown whether Ski is expressed and has a role in granulosa cells undergoing luteinization. Thus, the aim of the present study was to locate Ski protein in the rat ovary during luteinizationto predict the possible role of Ski. In order to examine the expression pattern of Ski protein along with the progress of luteinization, follicular growth was induced by administration of equine chorionic gonadtropin to immature female rats, and luteinization was induced by human chorionic gonadtropin treatment to mimic luteinizing hormone (LH) surge. While no Ski-positive granulosa cells were present in preovulatory follicle, Ski protein expression was induced in response to LH surge, and was maintained after the formation of the corpus luteum (CL). Though Ski protein is absent in granulosa cells of preovulatory follicle, its mRNA (c-Ski) was expressed and the level was unchanged even after LH surge. Taken together, these results demonstrated that Ski protein expression is induced in granulosa cells upon luteinization, and suggests that its expression is regulated post-transcriptionally.

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“…Gene expression of transforming growth factor α, which is synthesized by keratinocytes, and when included in the process of proliferation, stimulates the regeneration of tissues and wound healing (Coffey, 1995;Boilly et al, 2000;Vogel-Höpker et al, 2000;Pehlivan, 2001;Dörr et al,2002;Praul et al, 2002), and on the 20th and 35th day after irradiation in the area of the burn is very low. Administration of autofibroblasts does not alter anything.…”

Section: Table 1 Effect Of Autotransplantation Of Fibroblasts and The Mixture Of Fibroblasts And Keratinocytes On The Dynamics Of The Relmentioning

confidence: 99%

Biochemical mechanisms of skin radiation burns inhibition and healing by the volumetric autotransplantation of fibroblasts and of keratinocytes with fibroblasts composition

Altukhova

Kot

et al. 2015

VDNUBM

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Mechanisms of influence of volumetric autotransplantation of fibroblasts and of the mixture of fibroblasts and keratinocytes on the development of the local 3rd degree X-ray burn and the radiation skin ulcer in guinea pigs were investigated. We used deepadministration into the irradiation zone on its perimeter of 6 doses, which contained (150–160)×103 fibroblasts and (130–140)×103 keratinocytes in 100 µl. It is shown that this autotransplantation carried out 1 hour after the irradiation, and then every 24 hours, reduces the area of burn on the 35th day, compared to the control by 63%. Radiation ulcer appears on the 10th day after irradiation and is completely healed on the 25th day. With the same regimen of administration of only fibroblasts containing (200–210)×103 cells in 100 µl, these parameters of treatment were equal to 31% on 4th and 35th day, respectively. It is shown that as a result of radiation in the area of burn the level of gene expression of collagen types I and III, elastin, fibronectin, vinculin, decorin, hyaluronansynthases 1, 2, 3, matrix metalloproteinases 1, 2, 3, 7, 9 and hyaluronidase is reduced. Besides, in the burn area the level of gene expression of transforming growth factor α, fibroblast growth factors 1, 2, 8 and anti-inflammatory cytokines – interleukin 10 and transforming growth factor-β1 – is reduced, while the level of gene expression of proinflammatory cytokine (interleykin1β) increases. Both types of autotransplantation cause the growth of the expression level of all the structural genes and regulatory proteins of biopolymers and decrease in the expression level of interleukin 1β, which leads to activation of tissue regeneration and healing of the burn wound. Reasonsfor the higher efficiency of autotransplantation using the mixture of fibroblasts and keratinocytes compared to autotransplantation by fibroblasts only are both the larger total number of live cells regularly replacing dead cells in the burn area, and mutual stimulation of auto-fibroblasts and auto-keratinocytes to proliferate and to synthesize biologically active substances, i.e. cytokines and growth factors.

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Effects of transforming growth factors-alpha and -beta on proliferation and apoptosis of rat theca-interstitial cells

Abstract: Ovarian development, follicular growth and atresia require mechanisms regulating proliferation and death of ovarian cells including theca-interstitial (T-I) cells. Transforming growth factors-and -

Cited by 21 publications

References 53 publications

Smad 3 regulates proliferation of the mouse ovarian surface epithelium

Smad 3 regulates proliferation of the mouse ovarian surface epithelium

Induction of Ski Protein Expression upon Luteinization in Rat Granulosa Cells

Biochemical mechanisms of skin radiation burns inhibition and healing by the volumetric autotransplantation of fibroblasts and of keratinocytes with fibroblasts composition

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