Effects of trapezoid body and superior olive lesions on choline acetyltransferase activity in the rat cochlear nucleus

Godfrey, Donald A.; Park-Hellendall, Jami L.; Dunn, Jon D.; Ross, C. David

doi:10.1016/0378-5955(87)90053-0

Cited by 40 publications

(37 citation statements)

References 25 publications

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“…Their large number is sufficient to account for the amount of ChAT activity that disappears from the CN when the trapezoid body is completely cut, and their light staining for AChE activity may correlate with its smaller decline in the CN (Godfrey et al, 1983;Osen et al, 1984) than in the cochlear inner hair cell region (Godfrey and Ross, 1985) after transection of their centrifugal innervations. The larger number rostrally of the smaller ChAT-positive VNTB neurons could be consistent with the relatively larger effects on CN ChAT activity of lesions including more rostral damage (Godfrey et al, 1987b) and correlates with evidence for higher ChAT activities rostrally than caudally in the trapezoid body (Godfrey et al, 1988a).…”

Section: Chat-positive Vntb Neuronsmentioning

confidence: 72%

“…7,8) are about five times as numerous as the larger neurons. It is believed that they are, at least in part, responsible for the cholinergic projections to the CN via the trapezoid body route (Godfrey et al, 1987b) and do not project to the cochlea (Sherriff and Henderson, 1994). These neurons do not stain darkly for AChE (Fig.…”

Section: Chat-positive Vntb Neuronsmentioning

confidence: 98%

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Immunohistochemical evaluation of cholinergic neurons in the rat superior olivary complex

Yao

Godfrey

1998

Microsc. Res. Tech.

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Section: Chat-positive Vntb Neuronsmentioning

confidence: 72%

Section: Chat-positive Vntb Neuronsmentioning

confidence: 98%

Immunohistochemical evaluation of cholinergic neurons in the rat superior olivary complex

Yao

Godfrey

1998

Microsc. Res. Tech.

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“…Bar ϭ 100 m. duncular corner, and parts of the DCN molecular and deep layers, branching of individual fibers to provide multiple terminals, combined with relatively large terminal sizes, suggests that each cholinergic fiber may have a stronger influence here than elsewhere in the CN. The different patterns of cholinergic innervation in different CN regions may be associated with different origins (Godfrey et al 1987), including intrinsic neurons (Godfrey 1993;Vetter et al 1993), collaterals of the medial OCB (Benson and Brown 1990), and a population of smaller neurons of the ventral nucleus of the trapezoid body (VNTB) (Sherriff and Henderson 1994;Yao and Godfrey 1998). Alternatively, they may suggest subgroups within the VNTB population of smaller neurons, which appears to be the predominant source (Sherriff and Henderson 1994;Yao and Godfrey 1998).…”

Section: Discussionmentioning

confidence: 99%

Vesicular Acetylcholine Transporter in the Rat Cochlear Nucleus: An Immunohistochemical Study

Yao

Godfrey

1999

J Histochem Cytochem.

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SUMMARY After being synthesized in the cytoplasm of axon terminals, acetylcholine is packaged into synaptic vesicles by a proton-dependent transporter, vesicular acetylcholine transporter (VAChT). Localization of VAChT is restricted to cholinergic neurons, especially their terminals. We used an anti-VAChT antibody from INCSTAR to localize cholinergic terminals in the rat cochlear nucleus (CN), an important brainstem auditory center. VAChT immunoreactivity in the rat CN appears as labeled puncta and a few connecting fibers. In ventral CN (VCN), VAChT-labeled puncta are closely associated with somatic profiles of medium to large neurons. In and near the granular regions of VCN, VAChT-labeled puncta are more diffusely scattered. In the subpeduncular corner and the medial sheet, some VAChT-labeled fibers are seen in connection with especially prominent VAChT-labeled puncta. In dorsal CN (DCN), VAChT-labeled puncta show no clear association with somata and are found in all layers. Ultrastructurally, VAChT labeling is seen in the cytoplasm and is associated with synaptic vesicle membrane of terminals with small round vesicles. Such VAChT-labeled terminals synapse with cell bodies and dendrites in the CN.

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“…For example, it has been shown that cholinergic axons originating from the trapezoid nucleus [33] and from the superior olivary complex [11] terminate in the CN, and vesicular choline acetyltransferase positive boutons have been described to contact various cell types of the CN [43]. Moreover, expression of muscarinic receptors has been demonstrated, along with their expression patterns, in the CN [41,42].…”

Section: Introductionmentioning

confidence: 99%

Activation of muscarinic receptors increases the activity of the granule neurones of the rat dorsal cochlear nucleus—a calcium imaging study

Kőszeghy

Vincze

Rusznák

et al. 2012

Pflugers Arch - Eur J Physiol

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Acetylcholine modulates the function of the cochlear nucleus via several pathways. In this study the effects of cholinergic stimulation were studied on the cytoplasmic Ca 2+ concentration of granule neurones of the rat dorsal cochlear nucleus (DCN). Ca 2+ transients were recorded in Oregon-Green-BAPTA 1-loaded brain slices using a calcium imaging technique. For the detection, identification, and characterisation of the Ca 2+ transients, a wavelet analysis-based method was developed. Granule cells were identified on the basis of their size and localisation. The action potential-coupled character of the Ca 2+ transients of the granule cells was established by recording fluorescence changes and electrical activity simultaneously. Application of the cholinergic agonist carbamyl-choline (CCh) significantly increased the frequency of the Ca 2+ transients (from 0.37 to 6.31 min -1 , corresponding to a 17.1-fold increase; n = 89).This effect was antagonised by atropine, whereas CCh could still evoke an 8.3-fold increase of the frequency of the Ca 2+ transients when hexamethonium was present.Using immunolabelling, the expression of both type 1 and type 3 muscarinic receptors (M1 and M3 receptors, respectively) was demonstrated in the granule cells. Application of 1,1-dimethyl-4-diphenylacetoxypiperidinium iodide (an M3-specific antagonist) prevented the onset of the CCh effect, whereas an M1-specific antagonist (pirenzepine) was less effective. We conclude that cholinergic stimulation increases the activity of granule cells, mainly by acting on their M3 receptors. The modulation of the firing activity of the granule cells, in turn, may modify the firing of projection neurones, and may adjust signal processing in the entire DCN.

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Effects of trapezoid body and superior olive lesions on choline acetyltransferase activity in the rat cochlear nucleus

Cited by 40 publications

References 25 publications

Immunohistochemical evaluation of cholinergic neurons in the rat superior olivary complex

Immunohistochemical evaluation of cholinergic neurons in the rat superior olivary complex

Vesicular Acetylcholine Transporter in the Rat Cochlear Nucleus: An Immunohistochemical Study

Activation of muscarinic receptors increases the activity of the granule neurones of the rat dorsal cochlear nucleus—a calcium imaging study

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