Effects of treatment with Astragalus Membranaceus on function of rat leydig cells

Jiang, Xiaolong; Cao, Xia; Huang, Yang; Chen, Jianwei; Yao, Xiaolei; Zhao, Miaomiao; Yan, Long; Meng, Jinzhu; Li, Pengfei; Li, Zhiyan; Yao, Jianbo; Smith, George W.; Lv, Lei

doi:10.1186/s12906-015-0776-3

Cited by 19 publications

(14 citation statements)

References 26 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The results of the current study were similar to previous studies on tilapia (Shi et al, 2013), swimming crab, Portunus trituberculatus (Meng, Liu, Li, Gao, & Chen, 2014), and puffer fish (Cheng et al, 2017). To date, many studies have confirmed that A. membranaceus and its extracts have significant antioxidant activities (Jia et al, 2012;X. L. Jiang et al, 2014;M.…”

Section: Discussionsupporting

confidence: 90%

“…The results of the current study were similar to previous studies on tilapia (Shi et al, ), swimming crab, Portunus trituberculatus (Meng, Liu, Li, Gao, & Chen, ), and puffer fish (Cheng et al, ). To date, many studies have confirmed that A. membranaceus and its extracts have significant antioxidant activities (Jia et al, ; X. L. Jiang et al, ; M. Li et al, ). In this study, SOD, CAT, and GSH‐Px activities were significantly higher in the liver of AMEP‐supplemented groups than those in the NC group at 10°C, whereas the MDA content showed a reverse trend.…”

Section: Discussionmentioning

confidence: 96%

See 1 more Smart Citation

Improving low‐temperature stress tolerance of tilapia, Oreochromis niloticus: A functional analysis of Astragalus membranaceus

Yang

Huang

et al. 2019

J World Aquaculture Soc

View full text Add to dashboard Cite

A 54‐day experiment was conducted to investigate the effects of dietary supplementation of Astragalus membranaceus extract powder (AMEP) on the low temperature tolerance of genetically improved farmed tilapia, Oreochromis niloticus. The base diet was supplemented with 0.0% (NC), 0.1% (NP0.1), 0.2% (NP0.2), and 0.4% (NP0.4) AMEP. Tilapia with an initial body weight of 21.35 ± 0.17 g were fed three times a day for 14 days at 30°C and then fed for 40 days while the water temperature decreased at a rate of 0.5°C/day. The results showed that survival, final weight, and the rate of weight gain in fish in the NP0.1, NP0.2, and NP0.4 groups were higher than those of fish in the NC group. The contents of glucose, triglyceride, cholesterol, and nitric oxide in the serum of fish fed diets supplemented with AMEP were all significantly lower than those of fish in the NC group. In contrast, fish fed with AMEP‐supplemented diets had higher liver superoxide dismutase, glutathione peroxidase and catalase activities, and lower malonaldehyde content than those of fish in the NC group at 10°C. The expression of the growth‐arrest‐specific 2 gene in the liver was significantly lower in the NP0.2 and NP0.4 groups than in the NC group. The results of this study demonstrated that the optimal level of AMEP supplementation was 0.2% under the conditions used in the current study. Optimum dietary AMEP can promote growth performance and facilitate the survival of tilapia under low‐temperature stress.

show abstract

Section: Discussionsupporting

confidence: 90%

Section: Discussionmentioning

confidence: 96%

Improving low‐temperature stress tolerance of tilapia, Oreochromis niloticus: A functional analysis of Astragalus membranaceus

Yang

Huang

et al. 2019

J World Aquaculture Soc

View full text Add to dashboard Cite

show abstract

“…Astragaloside IV (AsIV) is one of the major active ingredients of the plant Astragalus membranaceus, which is referred to as HuangQi in Chinese. As an important Qi-invigorating medicinal herb, A. membranaceus is extensively used in traditional Chinese medicine for the treatment of cardiovascular diseases, hepatitis, and kidney and skin diseases (2,6,7). Chemically, AsIV is a cycloartane triterpene saponin with a well-defined molecular formula, the chemical structure of which is presented in Fig.…”

Section: Introductionmentioning

confidence: 99%

Astragaloside IV inhibits isoprenaline-induced cardiac fibrosis by targeting the reactive oxygen species/mitogen-activated protein kinase signaling axis

Dai

Jia

Lü

et al. 2017

Molecular Medicine Reports

View full text Add to dashboard Cite

Cardiac fibrosis is considered an important pathological mechanism in the progression of cardiac remodeling and heart failure. Astragaloside IV (AsIV) is a major active ingredient in Astragalus membranaceus. In a preliminary experiment, it was demonstrated that this naturally occurring substance exhibited cardioprotective effects via preventing cardiomyocyte hypertrophy and apoptosis. The present study aimed to investigate the effects of AsIV on β‑adrenergic receptor (β‑AR)‑mediated cardiac fibrosis, and the associated mechanism. Cell Counting Kit‑8 (CCK‑8) assay was used to examine the proliferation of rat cardiac fibroblast (CF) cultures. Collagen I secretion was detected by ELISA. Dihydroethidium was used to determine intracellular ROS levels. Western blotting was used to examine the expression level of total and phosphorylated mitogen‑activated protein kinases (MAPKs). In the present study, the effects of AsIV on β‑adrenergic receptor (β‑AR) ‑mediated cardiac fibrosis were investigated, and the associated mechanism was revealed. Isoprenaline (ISO) is a selective β‑AR agonist, and treatment with AsIV significantly inhibited (ISO)‑triggered cardiac fibroblast proliferation and type I collagen synthesis. In addition, ISO resulted in a significant elevation of reactive oxygen species (ROS) levels and phosphorylation of the three profibrotic MAPKs, namely extracellular signal‑regulated kinase, p38MAPK and c‑Jun N‑terminal kinase. AsIV effectively reversed the aforementioned ISO‑induced alterations. In addition, N‑acetylcysteine, a typical ROS scavenger, mimicked the inhibitory effects of AsIV on MAPK activation. The present study demonstrated that AsIV may inhibit ISO‑induced cardiac fibrosis by suppressing ROS‑mediated MAPK activation.

show abstract

“…Ta-65 exerted a partial preservation of the normal structure of the seminiferous tubules and Leydig interstitial cells. In vitro treatment of cultured Leydig cells with Ta-65 increased the number of Leydig cells and elevated the production of testosterone in the culture media (Jiang et al, 2015). Pomegranate stimulates GnRH from hypothalamus to increase synthesis and secretion of LH and FSH from the anterior pituitary (Hong et al, 2008).…”

Section: Discussionmentioning

confidence: 97%

Astragalus membranaceus and Punica granatum alleviate infertility and kidney dysfunction induced by aging in male rats

Alshinnawy¹,

El‐Sayed²,

Taha³

et al. 2020

Turk J Biol

View full text Add to dashboard Cite

Introduction Infertility affects up to 20% of couples globally (Pan et al., 2018). The risk of poor reproductive insufficiency in males increases at >35 years old due to age-related changes in aged males including high incidence of systemic diseases and infections, vascular insufficiency, reduced levels of sex hormones, mutations, disorders in the architecture of testes, and oxidative stress (Azenabor et al., 2015; Ilacqua et al., 2018). In men over 35 years old, negative changes in sperm quality are reported and become more pronounced with age over 40 years (Rosiak-Gill et al., 2019). By aging, deterioration in semen quality is observed in the ejaculate volume, sperm count, motility, vitality and morphology (Harris et al., 2011). Furthermore, aging is associated with loss of kidney function marked with progressive reductions in renal blood flow and glomerular filtration rate (GFR). The decline in GFR is attributed to reductions in glomerular capillary flow rate, glomerular capillary ultrafiltration coefficient, and afferent arteriolar resistance (Weinstein and Anderson, 2010). These changes lead to loss of renal mass; stiffness of afferent arterioles, sclerosis of glomeruli, and tubulointerstitial fibrosis (Yoon and Choi, 2014). Aging is associated with altered activity and responsiveness to vasoactive stimuli; responses to vasoconstrictor stimuli are enhanced, while vasodilatory responses are impaired. These changes may predispose the older kidney to acute and progressive chronic kidney injury (Weinstein and Anderson, 2010). Therefore, targeting the pathological changes associated with male infertility and kidney dysfunctions could reverse these aging-induced perturbations. Astragalus membranaceus is a Chinese medicinal herb used to treat various diseases and is marketed as life-prolonging extracts for human use in China (Liu et al., 2017). The major components of Astragalus membranaceus are polysaccharides, flavonoids, and saponins (Auyeung et al., 2016). The components of Astragalus membranaceus extract can increase telomerase activity, and has antiinflammatory,

show abstract

Effects of treatment with Astragalus Membranaceus on function of rat leydig cells

Cited by 19 publications

References 26 publications

Improving low‐temperature stress tolerance of tilapia, Oreochromis niloticus: A functional analysis of Astragalus membranaceus

Improving low‐temperature stress tolerance of tilapia, Oreochromis niloticus: A functional analysis of Astragalus membranaceus

Astragaloside IV inhibits isoprenaline-induced cardiac fibrosis by targeting the reactive oxygen species/mitogen-activated protein kinase signaling axis

Astragalus membranaceus and Punica granatum alleviate infertility and kidney dysfunction induced by aging in male rats

Contact Info

Product

Resources

About