Effects of treatment with sucrose in drinking water on liver histology, lipogenesis and lipogenic gene expression in rats fed high-fiber diet

Mašek, Tomislav; Filipović, Natalija; Vuica, Ana; Starčević, Kristina

doi:10.1016/j.plefa.2016.11.001

Cited by 20 publications

(22 citation statements)

References 28 publications

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“…Recently, substantial evidence emerged that palmitoleate could be considered as a lipokine . Moreover, palmitoleic acid content in tissue strongly responds to Δ9D expression with a decrease in IDDM, and an increase during metabolic syndrome, which makes it an interesting biomarker in metabolic diseases.…”

Section: Discussionmentioning

confidence: 99%

“…The quantification was performed by GC‐MS (QP2010 Ultra, Shimadzu, Kyoto, Japan) equipped with a BPX70 capillary column (0.25 mm internal diameter, 0.25 μm film thickness, 30 m long, SGE, Austin, TX, USA). Analytical conditions were set as described previously . Nonadecanoic fatty acid (C19:0) was used as an internal standard.…”

Section: Methodsmentioning

confidence: 99%

“…Total RNA was isolated from 30 mg of liver tissue and reverse transcribed as previously described with specific primers listed in Table S3, Supporting Information. The gene expression from each sample was analyzed and relatively quantified using the ∆∆Ct method (2 ∆∆Ct ) after normalization to β‐actin and cyclophilin (housekeeping genes) .…”

Section: Methodsmentioning

confidence: 99%

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Hepatic Lipogenesis and Brain Fatty Acid Profile in Response to Different Dietary n6/n3 Ratios and DHA/EPA Supplementation in Streptozotocin Treated Rats

Starčević

Filipović

Galán

et al. 2018

Molecular Nutrition Food Res

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Section: Discussionmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

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Hepatic Lipogenesis and Brain Fatty Acid Profile in Response to Different Dietary n6/n3 Ratios and DHA/EPA Supplementation in Streptozotocin Treated Rats

Starčević

Filipović

Galán

et al. 2018

Molecular Nutrition Food Res

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“…Fatty acids from TL and PL were transmethylated using 2M KOH in methanol. The analysis of fatty acid composition was performed as described previously (STARCEVIC et al, 2015, MAŠEK et al, 2017. All the experimental measurements were performed in triplicates and the average values reported.…”

Section: Methodsmentioning

confidence: 99%

“…In contrast, the content of essential C18 fatty acids, linoleic and linolenic was strongly decreased. Linoleic and linolenic acids must be supplied by the food and therefore, their decrease in metabolic syndrome is explained by partitioning into oxidation (EL HAFIDI et al, 2001, PEREZ et al, 2009, MAŠEK et al, 2017, MAŠEK and STARČEVIĆ, 2017b. In different models of metabolic syndrome the content of arachidonic acid (20:4n6) is usually decreased in hepatic tissue and serum , PEREZ et al, 2009.…”

Section: T Mašek Et Al: Influence Of Chronic Sucrose Intake On Rat mentioning

confidence: 99%

Chronic sucrose intake increases expression of SREBP-1c and inflammatory response genes in rat kidneys without significant changes in long chain polyunsaturated fatty acid content

et al. 2018

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________________________________________________________________________________________ MAŠEK, T., N. FILIPOVIĆ, A. GALAN, K. STARČEVIĆ:Chronic sucrose intake increases expression of SREBP-1c and inflammatory response genes in rat kidneys without significant changes in long chain polyunsaturated fatty acids content. Vet. arhiv 88, 497-510, 2018. ABSTRACTWe studied the influence of long-term treatment with sucrose in drinking water on the kidney histology, inflammation gene expression and lipogenesis. Male Wistar rats were supplemented with 30% sucrose (w/v) in water (MetSyn group) or with plain water (Control group). Kidney histology showed that the sucrose treated rats had increased renal lipid content and glomerulosclerosis. TGF-ß1 and TNF-α gene expression was also increased in the MetSyn group indicating low-grade inflammation. The fatty acid profile was characterized by increased saturated and monounsaturated fatty acids and a decrease in C18 essential fatty acids. The content of arachidonic and docosahexaenoic acids did not decrease despite the decrease in the content of their precursors. Neither the expression nor the protein quantity of delta-5 desaturase increased, indicating that renal delta-5-desatuaration is not important for the preservation of the kidney arachidonic and docosahexaenoic acid content in metabolic syndrome. The expression of SREBP-1c transcriptional factor increased two-fold in the MetSyn group, indicating increased renal lipogenesis in the sucrose treated rats. In conclusion, the results show that chronic sucrose intake induces glomerulosclerosis, low-grade inflammation, increases saturated and monounsaturated fatty acids and decreases C18 fatty acids without further decrease in arachidonic and docosahexaenoic fatty acids content.

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Reabsorption in the proximal tubuli—ultrastructural evidence for a novel aspect of renal VEGF trafficking

et al. 2018

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Many clinical and experimental studies have revealed VEGF as an important factor in the pathophysiology of renal damage during diabetes mellitus (DM). Anti-VEGF therapy is in clinical use for treatment of DM and other diabetes-related (and unrelated) diseases. Nevertheless, little is known about the metabolism of VEGF in the kidneys. In order to determine the ultrastructural localization of VEGF in the kidney, we study the distribution of VEGF in the kidney of rats by using immunogold immunohistochemistry. Our light-microscopic data showed remarkable re-distribution of VEGF in proximal tubular cells (PTCs) during prolonged hyperglycemia, a DM type 2 model (DM2), which was confirmed by transmission electron microscopy (TEM) findings. TEM findings revealed an initial presence of VEGF in the vesicular transport apparatus of PTCs in healthy rats and its gradual translocation to the apical membrane of PTCs after renal damage caused by high sucrose treatment. The presented data add to our understanding of kidney VEGF trafficking, providing novel insight into the renal metabolism and pharmacodynamics of the cytokine. This could have a high impact on the use of VEGF and anti-VEGF therapy in different renal diseases.

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Effects of treatment with sucrose in drinking water on liver histology, lipogenesis and lipogenic gene expression in rats fed high-fiber diet

Cited by 20 publications

References 28 publications

Hepatic Lipogenesis and Brain Fatty Acid Profile in Response to Different Dietary n6/n3 Ratios and DHA/EPA Supplementation in Streptozotocin Treated Rats

Hepatic Lipogenesis and Brain Fatty Acid Profile in Response to Different Dietary n6/n3 Ratios and DHA/EPA Supplementation in Streptozotocin Treated Rats

Chronic sucrose intake increases expression of SREBP-1c and inflammatory response genes in rat kidneys without significant changes in long chain polyunsaturated fatty acid content

Reabsorption in the proximal tubuli—ultrastructural evidence for a novel aspect of renal VEGF trafficking

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