1990
DOI: 10.1002/art.1780330412
|View full text |Cite
|
Sign up to set email alerts
|

Effects of tumor necrosis factor α and β on resorption of human articular cartilage and production of plasminogen activator by human articular chondrocytes

Abstract: We examined the effects of recombinant human tumor necrosis factor (TNF) on human articular cartilage and chondrocytes in culture. Both TNFa and TNFP stimulated cartilage matrix breakdown during prolonged culture and elevated the levels of plasminogen activator (PA) activity in both the supernatants and cell layers of cultured chondrocytes. Characterization of the PA activities by immunochemistry and by zymography following gel electrophoresis indicated that human chondrocytes produce both urokinase-type PA an… Show more

Help me understand this report

Search citation statements

Order By: Relevance

Paper Sections

Select...
3
1

Citation Types

4
49
1
1

Year Published

1992
1992
2004
2004

Publication Types

Select...
9

Relationship

2
7

Authors

Journals

citations
Cited by 98 publications
(55 citation statements)
references
References 34 publications
4
49
1
1
Order By: Relevance
“…Both IL-1p and TNFa are able to stimulate collagenase and prostaglandin E production and induce cartilage destruction in vitro (48)(49)(50), and they are arthritogenic in animal models (51,52). In addition, treatment of RA patients with anti-TNFa MAb has resulted in clinical improvement (53).…”
Section: Discussionmentioning
confidence: 99%
“…Both IL-1p and TNFa are able to stimulate collagenase and prostaglandin E production and induce cartilage destruction in vitro (48)(49)(50), and they are arthritogenic in animal models (51,52). In addition, treatment of RA patients with anti-TNFa MAb has resulted in clinical improvement (53).…”
Section: Discussionmentioning
confidence: 99%
“…These studies indicate that release of proteases with degrading activities is responsible for damaging the articular cartilage (Saklatvala, 1986). The secretion of these proteases is induced by several cytokines produced locally by macrophages and synovial cells (Dayer et al, 1985;Campbell et al, 1990). In addition, cytokines are especially important as mediators of inflammatory responses.…”
Section: Introductionmentioning
confidence: 99%
“…In addition, cytokines are especially important as mediators of inflammatory responses. Interleukin-1 (IL-1) and tumor necrosis factor (TNF) have been documented to contribute to joint destruction by induction of degrading proteases and by inducing synovial cells, synovial fibroblasts, and chondrocytes to secrete prostaglandin E 2 (Dayer et al, 1985;Campbell et al, 1990); these cytokines have also been associated with synovitis induced by endotoxin in horses (Price et al, 1992;Hawkins et al, 1993). During the initial phase of synovitis induced by intraarticular administration of endotoxin in horses, TNF was one of the first cytokines secreted into the joint fluid (Hawkins et al, 1993).…”
Section: Introductionmentioning
confidence: 99%
“…Cytokines have been implieated as mediators of both inflammation and joint destruction in rheumatoid arthritis (RA) [1][2][3][4]. They are released from activated T ceils and maerophages present in the joint, and may mediate their damage locally by causing synovial fibrablasts to release enzymes that damage cartilage and bone.…”
Section: Introductionmentioning
confidence: 99%