Effects of ubiquinol with fluid resuscitation following haemorrhagic shock on rat lungs, diaphragm, heart and kidneys

Abstract

New Findings r What is the central question of this study?What are the effects of administering ubiquinol (reduced form of co-enzyme Q 10 ) following haemorrhagic shock on leucocyte mitochondrial superoxide, diaphragmatic hydrogen peroxide concentration and apoptosis in the lungs, diaphragm, heart and kidneys? r What is the main finding and its importance?The administration of ubiquinol attenuated damage to the lungs, diaphragm, heart and kidneys following haemorrhagic shock and fluid resuscitation. Leucocyte mitochondrial superoxide was reduced, while hydrogen peroxide and apoptosis in the diaphragm decreased, as did apoptosis in the lungs, heart and kidneys. These findings suggest that ubiquinol could be a potential antioxidant used to reduce the reperfusion injury following haemorrhagic shock.Haemorrhagic shock (HS) and fluid resuscitation can lead to increased reactive oxygen species (ROS), contributing to ischaemia-reperfusion injury and organ damage. Ubiquinol is a potent antioxidant that decreases ROS. This study examined the effects of ubiquinol administered with fluid resuscitation following controlled HS. Adult male Sprague-Dawley rats were randomly assigned to treatment [ubiquinol, 1 mg (100 g body weight)−1 ] or control groups. Rats were subjected to 60 min of HS by removing 40% of the total blood volume to a mean arterial pressure ß45-55 mmHg. The animals were resuscitated with blood and lactated Ringer solution, with or without ubiquinol, and monitored for 120 min. At the end of the experiments, the rats were killed and the lungs, diaphragm, heart and kidneys harvested. Leucocytes were analysed for mitochondrial superoxide at baseline, end of shock and 120 min following fluid resuscitation using MitoSOX Red. Diaphragms were examined for hydrogen peroxide using dihydrofluorescein diacetate and confocal microscopy. The apoptosis in lungs, diaphragm, heart and kidneys was measured using fluorescence microscopy with acridine orange and ethidium bromide. Leucocyte mitochondrial superoxide levels were significantly lower in rats that received ubiquinol than in the control animals. Production of hydrogen peroxide and apoptosis were significantly reduced in the organs of rats treated with ubiquinol. These findings suggest that ubiquinol, administered with fluid resuscitation after HS, attenuates ROS production and

Ubiquinol decreases hemorrhagic shock/resuscitation-induced microvascular inflammation in rat mesenteric microcirculation

“…Frequent measurements of arterial blood gases were similar to our previous HS experiments with and without ubiquinol (Bennetts et al. ). In summary, arterial blood pH was unchanged in both groups throughout the experiment, as a result of hyperventilation compensating for the metabolic acidosis.…”

“…The results indicated animals receiving ubiquinol had significantly reduced levels of ROS and nuclear DNA damage (apoptosis) (Bennetts et al. ). In the present study, ubiquinol attenuated HS and FR induced microvascular inflammation by decreasing ROS levels, leukocyte adherence, mast cell degranulation, and vascular permeability.…”

Correlation between adiponectin and hemorrhagic shock in mice

“…HS is induced by many factors, including high rate of blood loss, severe vomiting or diarrhea, intestinal obstruction, or profuse sweating. Factors that result in rapid blood loss (over 20% of whole blood) will lead to shock, and death will occur quickly when the content of blood loss accounts for more than 50% of whole blood (Bennetts et al, 2014;Williams et al, 2014). HS is accompanied by many complications, the most evident of which is disseminated intravascular coagulation, which leads to the dysfunction of circulatory (and other internal organ) function, secondary fibrinolysis and consumptive coagulopathy.…”

Animal models in genomic research: Techniques, applications, and roles for nurses

“…In one study (Bennetts et al, 2014), two fluid resuscitation formulations (Lactated Ringer (LR) vs. LR + ubiquinol) were tested as a means of improving outcomes of hemorrhagic shock in rats. Hemorrhagic shock was induced by removing forty percent of the total blood volume, treatment administered, and animal monitored for two hours prior to sacrifice.…”

“…The heart, lungs, kidney, and diaphragm were harvested and analyzed. The main finding was that hydrogen peroxide levels and apoptotic proteins were reduced following LR + ubiquinol treatment, suggesting protective effects on oxidation and cell death (Bennetts et al, 2014). …”