Effects of ultrasonic treatment on the efficacy of gentamicin against established Pseudomonas aeruginosa biofilms

Huang, Chao-Yuan; James, Garth A.; Pitt, William G.; Stewart, Philip S.

doi:10.1016/0927-7765(96)01257-x

Cited by 21 publications

(7 citation statements)

References 24 publications

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“…This research differs significantly from previous work in our lab that was aimed at killing bacterial cells on surfaces or in suspension using the combination of ultrasound and antibiotics (32)(33)(34)(35)(36)(37)(38)(39)(40)(41). In that previous work, antibiotics are required to kill the cells during the exposure to ultrasound.…”

Section: Introductionmentioning

confidence: 71%

Ultrasound Increases the Rate of Bacterial Cell Growth

2003

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Ultrasound was employed to increase the growth rate of bacterial cells attached to surfaces. Staphylococcus epidermidis, Pseudomonas aeruginosa and Escherichia coli cells adhered to and grew on a polyethylene surface in the presence of ultrasound. It was found that low frequency ultrasound (70 kHz) of low acoustic intensity (<2 W/cm 2 ) increased the growth rate of the cells compared to growth without ultrasound. However, at high intensity levels, cells were partially removed from the surface. Ultrasound also enhanced planktonic growth of S. epidermidis and other planktonic bacteria. It is hypothesized that ultrasound increases the rate of transport of oxygen and nutrients to the cells and increases the rate of transport of waste products away from the cells, thus enhancing their growth.

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Section: Introductionmentioning

confidence: 71%

Ultrasound Increases the Rate of Bacterial Cell Growth

2003

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“…Numerous studies demonstrated an effect of ultrasound in combination with an antibiotic on bacterial populations (Zips et al. 1990; Huang et al. 1996; Qian et al.…”

Section: Discussionmentioning

confidence: 99%

Effect of pulsed ultrasound in combination with gentamicin on bacterial viability in biofilms on bone cements in vivo

Ensing

Roeder²,

Nelson

et al. 2005

J Appl Microbiol

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Aims: The aim of this study is to investigate whether pulsed ultrasound (US) in combination with gentamicin yields a decreased viability of bacteria in biofilms on bone cements in vivo. Methods and Results: Bacterial survival on bone cement in the presence and absence of ultrasound was compared in a rabbit model. Two bone cement samples with an Escherichia coli ATCC 10798 biofilm were implanted in a total of nine rabbits. In two groups bone cement discs loaded with gentamicin, freshly prepared and aged were used, and in one group unloaded bone cement discs in combination with systemically administered gentamicin. Pulsed ultrasound with a frequency of 28AE48 kHz and a maximum acoustic intensity of 500 mW cm )2 was applied continuously from 24 h till 72 h postsurgery on one of the two implanted discs.After euthanization and removal of the bacteria from the discs, the number of viable bacteria were quantified and skin samples were analysed for histopathological examination. Application of ultrasound, combined with gentamicin, reduced the viability of the biofilms in all three groups varying between 58 and 69% compared with the negative control. Histopathological examinations showed no skin lesions. Conclusions: Ultrasound resulted in a tendency of improved efficacy of gentamicin, either applied locally or systemically. Usage of ultrasound in this model proved to be safe. Significance and Impact of the Study: This study implies that ultrasound could improve the prevention of infection immediately after surgery, especially because the biomaterials, gentamicin and ultrasound used in this model are all in clinical usage, but not yet combined in clinical practice.

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“…Електричні поля низької напруги підвищували ефективність антисептиків, їх бактерицидна концентрація на біоплівкові форми бактерій нижча, ніж мінімальна бактерицидна концентрація для планктонних форм. Дослідники (Huang et al, 1996) продемонстрували ефективність ультразвуку щодо біоплівок P. aeruginosaобробка підвищувала дію гентаміцину відносно тих самих біоплівок. Таким чином, лабораторні мікробіологічні дослідження вказують, що використання електрофорезу з діоксизолем для лікування ХАТ перспективне та актуальне.…”

Section: обговоренняunclassified

The effect of antimicrobial agents on planktonic and biofilm forms of bacteria that are isolated from chronic anal fissures

Kozlovska

Romanjuk

et al. 2017

Regul. Mech. Biosyst.

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The microorganisms that are formed in biofilm cause about 60% of chronic and recurrent diseases, and as a consequence, traditional etiotropic antibacterial therapy is ineffective. Chronic anal fissures are also a disease which is caused by biofilm forms of bacteria, has a chronic course and is difficult to treat. The sensitivity of planktonic and biofilm forms of bacteria isolated from chronic anal fissures to antibacterial drugs was determined and the method of degradation of biofilm by electrophoresis for the effective treatment of fissures was developed. It was found that the most effective antibiotics against planktonic forms of bacteria were cephalosporins III and IV generations: cefеpime, cefoperazone and ceftazidime. Exceptionally, only bacteria of the genus Enterococcus, which were sensitive to ceftazidime, were found to be 38.9%. The sensitivity of the bacteria to Furamag was from 60.0% to 100.0%, and only P. aeruginosa exhibited resistance in 100.0% of the studied cultures. The number of sensitive to gatifloxacin strains of P. aeruginosa and Enterobacter spp. was 71.4%, all other isolated bacteria were sensitive to this preparation from 77.8% to 100.0%. Among the five studied antiseptics (chlorhexidine, decasan, octinisept, povidone iodine, dioxidine), the greatest antimicrobial activity was found in dioxidine and betadine (povidone iodine) solutions, the sensitivity of the microflora was from 60.0% to 100.0%. We found that the most protected biofilm matrix was P. aeruginosa and Enterococcus spp. We found that the antibiotic which had the best effect on cells in biofilm was fluoroquinoione gatifloxacin. After its influence on the biofilm P. aeruginosa and Enterococcus spp., the number of living cells didn’t exceed lg 1.5 ± 0.02 CFU/cm2 in the area of the biofilm, and S. aureus and E. coli cells were completely inactivated. After the influence of other antibiotics, the number of microbial cells that survived in the biofilm did not exceed lg 2.9 ± 1.6 CFU/cm2 of the area. It was found that after the action of dioxin, the amount of viable microbial cells was up to lg 2.9 ± 1.7 CFU/cm2 of biofilm area. Antiseptics: octine septum, ranopost, decaSan and chlorhexidine exhibited less strong bactericidal action on cells in biofilms, and the number of bacteria that survived after their action ranged from 2.9 ± 1.8 to lg 3.7 ± 2.1 CFU/cm2 of biofilm area. We propose using solution "Dioxysol-Darnitsa" (active substance dioxidine) for local treatment of patients with chronic anal fissures for intracutaneous electrophoresis of the fissure. We established that under the influence of electrophoresis at a current of 0.05–0.10 mA/cm2 of the area of the biofilm with dioxidine, bacteria were not isolated. This indicates on the destruction of the matrix and the effective contact of dioxidine with microbial cells and the manifestation of bactericidal action. Consequently, laboratory microbiological studies indicate that the use of electrophoresis with dioxysole in the treatment of chronic anal fissures is promising.

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Effects of ultrasonic treatment on the efficacy of gentamicin against established Pseudomonas aeruginosa biofilms

Cited by 21 publications

References 24 publications

Ultrasound Increases the Rate of Bacterial Cell Growth

Ultrasound Increases the Rate of Bacterial Cell Growth

Effect of pulsed ultrasound in combination with gentamicin on bacterial viability in biofilms on bone cements in vivo

The effect of antimicrobial agents on planktonic and biofilm forms of bacteria that are isolated from chronic anal fissures

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