Effects of Ultrasonic Treatment on the Physicochemical Properties and DPPH Radical Scavenging Activity of Polysaccharides from Mushroom Inonotus obliquus

Abstract

Polysaccharides from Inonotus obliquus exhibited many biological activities including antitumor, antioxidant, hypoglycemic, immune-stimulating effects, and so on. Ultrasonic treatment is now an usual proceeding technique for the extraction of some bioactive constituents. But there was no information about the effects of ultrasonic treatment on the physicochemical properties and DPPH radical scavenging activity of polysaccharides from I. obliquus until now. Ultrasonic technique was an effective method to extract bioactive polysaccharides from I. obliquus (UPS) according to this study.

Physical modifications of polysaccharide from Inonotus obliquus and the antioxidant properties

“…It could be inferred that the cleavage of protein and the rupture of main chain accounted for the decrease of Mw value upon thermal treatment. The molecular weight of Ul-IOPS obviously decreased from 12.20 Da ×10 4 Da to 6.88 Da ×10 4 Da and 2.98 Da × 10 4 Da, suggesting that the molecular weight of the ultrasonic treatment polysaccharides was lower than that of extraction by hot water, which could be explained as that ultrasonic wave could degrade high molecular weight polysaccharides to small ones [14].…”

“…The polysaccharides of I. obliquus were extracted based on the method reported by Fu et al [14]. Briefly, the sclerotia of I. obliquus were refluxed with 80% (v/v) ethanol at 80 • C for 2 h for three times.…”

“…In Chen's study, it was postulated that thermal effects of tea acid polysaccharide conjugate on the homogeneity and conformation were responsible for the change of antioxidant activity [18]. Fu found that ultrasonic technique was an effective method to extract bioactive polysaccharides from I. obliquus (UPS) [14]. Most of the studies only refer to the optimization of extraction or bioactivities for IOPS.…”

“…A number of studies had shown that polysaccharides from I. obliquus (IOPS) could possess significant antioxidant activities [6][7][8]. IOPS had attracted special research interest for its health-promoting effects including anticancer effects [9], immunostimulating activity [10], hypoglycemic effects [11], antilipidperoxidative effects [12], anti-inflammatory action [13], and antioxidant properties [14]. In the structure-activity studies of polysaccharides, the antioxidant capacity might be hindered by spatial structures whereas low molecular weight polysaccharide might be more effective.…”

Physicochemical characteristics of ultrasonic extracted polysaccharides from cordyceps cephalosporium mycelia

“…As it was reported, extended proportion of crystalline regions in polysaccharides lead to an increase in the peak temperature and enthalpy value for removing moisture and degradation [18,19]. Changes in thermodynamic as well as morphological characteristics of USEP might be due to the more compact arrangement in polysaccharides, which was induced by ultrasonic treatment.…”