Effects of Ultrasound‐Assisted Alkaline Extraction on Antioxidant Activity and Functional Characteristics of Chicken Feather Keratin Peptides

Abstract

In this research, we focus on enzymatic hydrolysis of chicken feathers, after chemical or chemical and ultrasound pretreatment. Our result illustrated that ultrasound pretreatment preserved the main structure and amino acid composition of extracted keratin as confirmed by the Fourier-transform infrared (FTIR) spectroscopy and gel-electrophoresis analysis. In addition, investigation showed that ultrasound-assisted chemical treatment was more effective on enzymatic hydrolysis and bioactivity as compared to only the chemical treatment. Thermo gravimetric analysis (TGA) characterization of hydro-lyzed products, using Alcalase enzyme at different times, showed that decomposition temperature was decreased during enzymatic hydrolysis and the best antioxidant activity was achieved after 30 min of hydrolysis with the value of 90.43 � 4.03 % (5 mg/ml concentration). Also, it was found that hydrolyzed product indicated no cytotoxicity to SK-N-MC cells when the concentration is less than 0.312 mg/ml. In this paper, the results demonstrated that the ultrasonic-assisted enzymolysis processing seems to be effective for producing keratin antioxidant hydrolysate.

Reactive Keratin: Harnessing the Power of Polymer Solutions Extracted Hydrothermally from Poultry Feathers and Wool for Eco-Friendly Functional Finishes on Polyester Textiles

“…High levels of such sulphur-containing amino acids, particularly cysteine, have been suggested to account for the antioxidant activity of the feather keratin. The antioxidant properties have been attributed directly to disruption of the disul de bonds during keratinous material solubilisation, therefore freeing the highly reactive thiol functional groups (SH), that could act as scavengers to cellular oxidants [40]. It has been shown that the antioxidant action of protein hydrolysates involves such mechanisms as deactivation of reactive oxygen species, reduction of hydroperoxides, chelation of prooxidant metallic ions, and changes in the physical properties of food systems [41,42], pollutants and environmental toxins.…”

Enhancing activity of food protein‐derived peptides: An overview of pretreatment, preparation, and modification methods

“…Remarkably, bioactive peptides and proteins demonstrate properties akin to hormones or drugs, thereby playing a beneficial role in metabolic processes and contributing to human well‐being. Bioactive peptides are categorized based on their distinct biological activities, including but not limited to antihypertensive (Chen et al., 2023; Uluko et al., 2014), antioxidant (Alahyaribeik & Ullah, 2020; Alizadeh & Aliakbarlu, 2020; Ashraf et al., 2020), antibacterial (Aguilar‐Toala et al., 2020; Alizadeh & Aliakbarlu, 2020), immunomodulatory (Zhang, Huang, et al., 2019c), anticancer (Chan et al., 2011; Ma et al., 2022), hypocholesterolemic (Ashraf et al., 2020), and mineral‐binding peptides (Luo et al., 2022).…”

Human hair proteins as natural reactive oxygen species scavengers for in vitro applications

“…Indeed, chemical reduction, enzymatic extraction, and ultrasound-assisted alkaline extraction have been demonstrated in recent studies utilizing chicken feathers to produce keratin hydrolysates with antioxidant and antibacterial activities. [11][12][13][14] The antioxidant properties have been attributed to disruption of the disulfide bonds during keratinous material solubilization, therefore freeing the highly reactive thiol functional groups ( SH) that could act as scavengers to cellular oxidants. Furthermore, bioactivities of antioxidant peptides isolated from poultry keratins were also scrutinized.…”