Effects of UVB radiation and salt stress on growth, pigments and antioxidative defence of the marine diatom Cylindrotheca closterium

Rijstenbil, J.W.

doi:10.3354/meps254037

Cited by 78 publications

(56 citation statements)

References 60 publications

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“…Such a high ratio is generally found in diatoms from subtidal, rather than intertidal, sediments, where it is associated with low irradiance (Cibic et al 2007, and references therein). Phytoplankton diatom communities also often display ratios of fucoxanthin:chl a > 1, depending on irradiation, nutrient regime and life stage (Schlüter et al 2000, Henriksen et al 2002, but to our knowledge, this has not been found for intertidal benthic diatoms (Rijstenbil 2003). In addition to the elevated fucoxanthin:chl a ratios, pheophytin a made up to 70% of the pigment composition in the dry area at Seokmo Island.…”

Section: Photosynthetic Pigment Abundance and Distributionmentioning

confidence: 98%

Section: Photosynthetic Pigment Abundance and Distributionmentioning

confidence: 99%

“…At all of these stations, diadinoxanthin was negatively correlated with diatoxanthin (Pearson correlation, r 2 = 0.494, p < 0.01; Hampyeong Bay: r 2 = 0.375, p < 0.01). Diadinoxanthin can be transformed into the heat-dissipating diatoxanthin in benthic algal communities for photoprotection (Demers et al 1991, Rijstenbil 2003, which could indicate a higher radiation stress of the microphytobenthos at the dry areas during emersion.Generally, microphytobenthos is associated with muddy sediments, which are common in sheltered environments and provide nutrient-rich substrates (Underwood & Kromkamp 1999, Facca et al 2002. Seokmo Island and Dolmori 1 had low mud contents and high chl a concentrations in the seepage areas, and at Songseokri Stn, with the lowest mud content, the chl a concentrations in the seepage areas exceeded those of all other studied sites (Fig.…”

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confidence: 99%

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Differences in microphytobenthos and macrofaunal abundances associated with groundwater discharge in the intertidal zone

Waska¹,

Kim²

2010

Mar. Ecol. Prog. Ser.

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We investigated the impact of intertidal groundwater seepage on benthic microalgae and macrofauna in 4 study sites located in 2 large tidal flat ecosystems along the western coast of Korea by comparing the chemical, physical, and biological characteristics of 'glossy' seepage sites with those of nearby areas without visually distinct groundwater discharge (dry sediment surface). At 3 of the 4 sites, sediment properties as well as pore water chemistry were similar in groundwater seepage and dry areas. At the 4th study site, the groundwater seepage areas were more coarsegrained compared to the dry areas. Here, the groundwater seepage also had lower salinity and higher nutrient concentrations than the pore water of the dry area and the seawater in a nearby tide pool. Although diatoms were the dominant algal class in seepage and dry areas alike, the seepage areas in 3 of the sites had elevated contributions of other marker pigments such as chlorophyll (chl) b compared to the dry areas. Chl a concentrations were higher in all seepage areas compared to dry areas, and all dry areas had high pheophytin a:chl a ratios, indicating a substantial amount of degraded algal material. In the seepage areas of 3 of the sites, we found large numbers of the snail Batillaria cumingi, while crab burrows of Scopimera sp. were only present in the neighboring dry areas. Correlations of sediment chl a concentrations with physicochemical properties of the ambient pore water indicated that microphytobenthos responded specifically to groundwater seepage, which may provide shelter from desiccation and salt stress during emersion of the tidal flat. Our results suggest that globally common groundwater seepage significantly impacts the ecosystem structures and microphytobenthos production of the tidal flats.KEY WORDS: Groundwater · Seepage · Microphytobenthos · Macrofauna · Intertidal · Pigments · Tidal flat Resale or republication not permitted without written consent of the publisherMar Ecol Prog Ser 407: [159][160][161][162][163][164][165][166][167][168][169][170][171][172] 2010 Intertidal microphytobenthos, i.e. the assemblage of bottom-living microalgae such as diatoms, is an important contributor to the benthic food chain (MacIntyre et al. 1996, Kang et al. 2003. Through resuspension, microphytobenthos can be transferred to pelagic systems as well (De Jonge & van Beusekom 1992). Furthermore, microphytobenthos can regulate diffusive nutrient fluxes between sediment and the overlying water column (Sundbäck et al. 1991). The relationship between microphytobenthos and ambient nutrient concentrations is often unclear. Generally, benthic microalgae, particularly from muddy sediments, are not nutrient limited (Underwood & Kromkamp 1999), and nutrient enrichment via the water column does not seem to have any effect on microphytobenthos biomass (Hillebrand & Kahlert 2002). On the other hand, microphytobenthos cultures can be maintained well with natural porewater (Admiraal et al. 1982), and microphytobenthos mats in the sandy sediment...

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Section: Photosynthetic Pigment Abundance and Distributionmentioning

confidence: 98%

Section: Photosynthetic Pigment Abundance and Distributionmentioning

confidence: 99%

mentioning

confidence: 99%

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Differences in microphytobenthos and macrofaunal abundances associated with groundwater discharge in the intertidal zone

Waska¹,

Kim²

2010

Mar. Ecol. Prog. Ser.

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“…Pigments were separated on a reverse-phase column (Nova-pak C18; Waters Millennium HPLC system). Gradient mixing pumps delivered 3 mobilephase solvents: methanol/ammonium acetate, 90% acetonitril and 100% ethyl acetate (Rijstenbil 2003 and references therein). Pigments were identified and quantified by commercially available standards using both a fluorescence detector (Waters 474 Scanning Fluorescence Detector) and a photodiode array absorption detector (Waters 996 Photodiode Array Detector).…”

Section: Methodsmentioning

confidence: 99%

Phospholipid-derived fatty acids as chemotaxonomic markers for phytoplankton: application for inferring phytoplankton composition

Dijkman¹,

Kromkamp²

2006

Mar. Ecol. Prog. Ser.

128

136

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Phospholipid-derived fatty acids (PLFA) are widely used as chemotaxonomic markers in microbial ecology. In this paper we explore the use of PLFA as chemotaxonomic markers for phytoplankton species. The PLFA composition was determined for 23 species relevant to estuarine phytoplankton. The taxonomic groups investigated were Chlorophyceae, Trebouxiophyceae, Prasinophyceae, Bacillariophyceae, Dinophyceae, Cryptophyceae, Rhodophyceae, Pavlovophyceae and Prymnesiophyceae. Most of these taxonomic groups have a characteristic PLFA composition, although a few contain truly unique PLFA biomarkers. The PLFA composition was used to derive species composition and abundance of the phytoplankton in the Scheldt estuary (border region between Belgium and the Netherlands) in April, July and October 2003, using the matrix factorization program CHEMTAX. The results agree well with results from HPLC-derived pigment analysis of the same samples. The advantages of using PLFA instead of pigments, or rather in addition to pigments, are that (1) information on bacteria can be derived in addition to information on photosynthetic taxa, (2) in some cases more or different taxa can be distinguished and (3) the isotopic composition of PLFA can be measured relatively easily, allowing the measurement of group-specific primary production.KEY WORDS: Phytoplankton composition · Phospholipid-derived fatty acids · PLFA · Scheldt estuary · Biomarkers · CHEMTAX Resale or republication not permitted without written consent of the publisherMar Ecol Prog Ser 324: [113][114][115][116][117][118][119][120][121][122][123][124][125] 2006 microscopic enumerations require a trained microscopist and are time-consuming. Furthermore, in most cases, the algae have to be stored and therefore fixed before analysis and not all taxa withstand fixation well (Gieskes & Kraay 1983). Often, cell numbers are difficult to compare due to large differences in cell size and volume and cell numbers have to be converted to biovolume (e.g. Hillebrand et al. 1999) or carbon (Menden-Deuer & Lessard 2000), a tedious procedure.The use of chemotaxonomic markers provides an alternative method for determining phytoplankton composition. Photosynthetic pigments are the best known chemotaxonomic markers for phytoplankton. As HPLC technology advances, more and more pigments can be routinely measured, allowing an increasing number of taxa to be distinguished (e.g. Zapata et al. 2004, Jeffrey & Wright 2006. In recent years, several studies have compared cell numbers and pigments, and in general a good agreement is found between these 2 methods both in freshwater and marine ecosystems (e.g. Garibotti et al. 2003, Marinho & Rodrigues 2003. Even groups lacking specific pigments can be detected with the help of pigment ratios using matrix factorization programs such as CHEMTAX (Mackey et al. 1996, Schlüter & Møh-lenberg 2003.Bacteria are also an important constituent of aquatic ecosystems. Suitable pigment biomarkers are lacking for bacteria, and phospholipid-derived fatty acids (PLF...

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“…Pigments were extracted in 90% acetone and analysed by HPLC using a reversed-phase analytical column (Nova pak C 18 , 4 mm, 15 cm; Waters Millenium HPLC system), as described in Rijstenbil (2003). The matrix factorization program CHEMTAX (Mackey et al, 1996) was used to estimate algal group abundance from the pigment composition as in Dijkman & Kromkamp (2006).…”

Section: Pigment Composition and Suspended Particulate Mattermentioning

confidence: 99%

Photosynthetic characteristics of the phytoplankton in the Scheldt estuary: community and single-cell fluorescence measurements

Dijkman

Kromkamp

2006

European Journal of Phycology

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Estuaries present a very dynamic environment for phytoplankton with large changes in salinity occurring over relatively short distances, usually with high turbidity. We investigated the photosynthetic characteristics of phytoplankton in the Scheldt estuary (Belgium and the Netherlands) along a salinity gradient using community and single-cell fluorescence measurements. Single-cell fluorescence measurements offer the opportunity to study differences between species that were formerly possible only using uni-algal cultures. The objective was to study differences in the photochemical efficiency of photosystem II (F v /F m ) between different species or different genera (when identification to the species level was not possible) and to investigate the effect of salinity on individual species. The difference in F v /F m between species was small, but often significant (ANOVA, p < 0.05), and was found to be a true characteristic of species, as opposed to being caused by stochastic processes affecting cells of all species more or less equally. High algal mortality is implied by a large decline of biomass from freshwater towards higher salinity, as well as a complete change in the phytoplankton composition. Nevertheless, cells with low F v /F m , which would indicate decreased health, were rare. F v /F m of 93% of the measured cells was between 0.5 and 0.8. There was no obvious relation between F v /F m and species abundance. In laboratory studies, green algae are often reported to have a higher F v /F m than diatoms, but we found no indication of this in the Scheldt estuary.

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Effects of UVB radiation and salt stress on growth, pigments and antioxidative defence of the marine diatom Cylindrotheca closterium

Cited by 78 publications

References 60 publications

Differences in microphytobenthos and macrofaunal abundances associated with groundwater discharge in the intertidal zone

Differences in microphytobenthos and macrofaunal abundances associated with groundwater discharge in the intertidal zone

Phospholipid-derived fatty acids as chemotaxonomic markers for phytoplankton: application for inferring phytoplankton composition

Photosynthetic characteristics of the phytoplankton in the Scheldt estuary: community and single-cell fluorescence measurements

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