Effects of vanadate on mechanical and electrical activities in guinea-pig taenia coli.

Ueda, Fukiko; Kishimoto, Tadashi; Ozaki, Hiroshi; Karaki, Hideaki; Urakawa, Norimoto

doi:10.1540/jsmr1965.20.85

Cited by 4 publications

(3 citation statements)

References 22 publications

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“…Further, the cells could not fix on the surface of flask at higher concentration than 10 −5 M, and the change of shapes was observed. VAN has inhibitory effects on various ATPase such as Na + /K + -ATPase and Ca-ATPase, and thereby, complicated phenomena such as contraction and relaxation are observed in various tissues [13][14][15][16]. Although the reason for the none fixation in high concentration of VAN was not investigated in the present study owing to the deviation from the aim of this study, more study will be needed to know the mechanisms of VAN in details.…”

Section: Discussionmentioning

confidence: 87%

“…In the experiment, the cells were treated with 10 −3 M VAN, because this concentration of VAN produced the maximum contractions in NT solution and relaxations in high potassium solution, in various isolated smooth muscles such as vascular, intestinal, and tracheal muscles [13][14][15][16] without change in PH of NT solution. Consequently, we obtained the V distributions of Vero cell in the VAN solution, extracellular and intracellular, as 1:0.564:0.036 and 1:0.662:0.098 at 60 and 120 min.…”

Section: Discussionmentioning

confidence: 99%

“…On the other hand, physiological effects may relate to the inhibitory effects on the Na + /K + -ATPase [7][8][9] and on the Ca 2+ -ATPase [10][11][12]. We have also reported that one of V compounds, vanadate (VAN), has the inhibitory effects on the contractions induced by high concentration of potassium in isolated tissues from animals, such as taenia coli [13,14], vascular smooth muscle [15], and tracheal smooth muscle [16]. The inhibitory effects were dependent on the cellular V contents [17][18][19].…”

Section: Introductionmentioning

confidence: 99%

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A Basic Study on the Biological Monitoring for Vanadium—Effects of Vanadium on Vero Cells and the Evaluation of Intracellular Vanadium Contents

Mochizuki

Kudo

Kikuchi

et al. 2010

Biol Trace Elem Res

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A high concentration of vanadium (V) has toxic effects on human and animals and is one of environmental pollutants. In the present study, we have conducted a fundamental study using cultured Vero cells from monkey kidney for the future environmental monitoring. Orthovanadate (VAN), one of V compounds, of 10(-10) and 10(-8) M did not affect the cell growth although the higher concentration of above 10(-6) M VAN inhibited the cell growth accompanied with the decrease in cell numbers and morphological changes. Given that the washing method with ice-cold Li is also effective for determination of the cellular Na content, we used this method for the determination of the V content of the Vero cells. The V distributions in Vero cell; in the 10(-3) M VAN solution, extracellular and intracellular were obtained as 1:0.564:0.036 and 1:0.662:0.098 at 60 and 120 min after the treatment of VAN. The intracellular V content was 10% of the applied concentration of VAN. Consequently, it was suggested that V concentration of 10(-7) and 10(-6) M in the tissue and environment, respectively, might become the threshold concentration; a criterion of the environmental contamination when we carry out environmental monitoring.

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Section: Discussionmentioning

confidence: 87%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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A Basic Study on the Biological Monitoring for Vanadium—Effects of Vanadium on Vero Cells and the Evaluation of Intracellular Vanadium Contents

Mochizuki

Kudo

Kikuchi

et al. 2010

Biol Trace Elem Res

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Vanadate treatment reverses gastrointestinal complications in the streptozotocin-diabetic rats

Öztürk

Arı

Pekiner

et al. 1994

General Pharmacology: The Vascular System

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Relationship between vanadate induced relaxation and vanadium content in guinea pig taenia coli

Mochizuki¹,

Ueda²,

Sano³

et al. 2000

Rev. can. physiol. pharmacol.

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Vanadate has been known to induce a transient increase in high K+ induced contraction, and also gradually relax the high K+ contraction itself in guinea pig taenia coli. The relationship between the rate of relaxation and ion content of Na+, K+, and V ion at the cellular level was investigated when vanadate was applied to contracted muscle. Tissue Na+ and V ion content increased linearly, depending on the time after vanadate treatment, reaching maximum levels of approximately 50 mM x kg(-1) and 0.25 mM x kg(-1) wet weight, respectively. There was a positive correlation between the V ion and Na+ contents, while there was a negative correlation between both ions and the relaxed rate of the high K+ induced contraction. The uptake of V ion was affected by the external K+ concentration, and the maximum rate of V ion uptake decreased to 40% in the presence of 90 mM external K+. These results suggest that a small amount of V ion was enough to inhibit the Na+ pump activity and muscle contraction in the high K+ solution.

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Effects of vanadate on mechanical and electrical activities in guinea-pig taenia coli.

Cited by 4 publications

References 22 publications

A Basic Study on the Biological Monitoring for Vanadium—Effects of Vanadium on Vero Cells and the Evaluation of Intracellular Vanadium Contents

A Basic Study on the Biological Monitoring for Vanadium—Effects of Vanadium on Vero Cells and the Evaluation of Intracellular Vanadium Contents

Vanadate treatment reverses gastrointestinal complications in the streptozotocin-diabetic rats

Relationship between vanadate induced relaxation and vanadium content in guinea pig taenia coli

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