Effects of varying degrees of partial bladder outlet obstruction on urinary bladder function of rats: A novel link to inflammation, oxidative stress and hypoxia

Kaya‐Sezginer, Ecem; Yilmaz‐Oral, Didem; Lokman, Utku; Nebioğlu, Serpil; Aktan, Fügen; Gür, Serap

doi:10.1111/luts.12211

Cited by 22 publications

(33 citation statements)

References 47 publications

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“…We agree that further detailed and systematic examinations of the relationships between degree, type, and duration of urinary dysfunction and each oxidative stress biomarker in various samples such as urine, blood, and bladder tissues are needed [80]. For example, in rats, a severe PBOO model with a 3Fr catheter (diameter 1 mm) showed increasing levels of MDA in bladder tissue compared to the control, whereas an intermediate PBOO model with a 4Fr catheter (1.3 mm) showed no significant difference [46]. In addition, as shown in Table 2, there are few biomarkers of oxidative stress in urine other than 8-OHdG.…”

Section: Problems To Be Solved and Future Directionsmentioning

confidence: 99%

A Review of Oxidative Stress and Urinary Dysfunction Caused by Bladder Outlet Obstruction and Treatments Using Antioxidants

et al. 2019

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Urinary dysfunction is a common pathological condition that can significantly decrease the quality of life. Bladder outlet obstruction (BOO) is a major cause of urinary dysfunction, and various lower urinary tract diseases including benign prostatic hyperplasia and urethral stricture disease cause BOO. According to the results of a variety of animal experiments on partial BOO (PBOO), there is a general agreement that ischemic conditions and repeated ischemia/reperfusion of the bladder are closely associated with BOO-induced bladder damage, and that increased oxidative stress by ischemia/reperfusion plays a crucial role in the pathological mechanisms underlying urinary dysfunction. Changes in biomarkers of oxidative stress in PBOO animal models support this association between oxidative stress and urinary dysfunction. Oxidative stress is defined as an imbalance between the production of pro-oxidants, such as free radicals and reactive species, and their elimination through protective mechanisms of antioxidants. Therefore, organizing the knowledge on the state of oxidative stress, changes in biomarkers, and biological roles of antioxidants in systemic and bladder tissues is essential to understand the detailed pathological characteristics of the urinary dysfunction caused by PBOO. Furthermore, information on drugs and supplements that have antioxidant effects is important for defining treatment strategies for urinary dysfunction with PBOO. In this review, we paid special attention to the following three issues; (1) changes in oxidative stress, including its biomarkers, (2) antioxidant status, and (3) previous reports on treatment strategies involving agents with antioxidative activity for urinary dysfunction caused by BOO. In particular, we provide systematic information on the detailed mechanisms underlying the antioxidative effects of agents used to treat PBOO. In addition, we show present research issues and research limitations, as well as suggest possible future antioxidant treatment strategies for patients with PBOO.

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Section: Problems To Be Solved and Future Directionsmentioning

confidence: 99%

A Review of Oxidative Stress and Urinary Dysfunction Caused by Bladder Outlet Obstruction and Treatments Using Antioxidants

et al. 2019

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“…The anti-oxidative and anti-inflammatory properties of Lycogen™ have been demonstrated when mounting our prior investigations [ 1 , 2 , 7 , 31 , 32 , 49 , 53 ]. Lycogen™ treatment was demonstrated to inhibit NO production and inducible nitric-oxide synthase (iNOS) expression in activated macrophages [ 1 ].…”

Section: Discussionmentioning

confidence: 59%

“…( c ) Postulated mechanisms of how Lycogen may reduce BPH. References utilized in this composite figure include [ 1 , 2 , 6 , 7 , 30 , 31 , 32 ]. Red arrow represented up-regulation of gene/protein level; blue arrow represented down-regulation of gene/protein level.…”

Section: Figurementioning

confidence: 99%

Rhodobacter sphaeroides Extract Lycogen™ Attenuates Testosterone-Induced Benign Prostate Hyperplasia in Rats

Wang

Liu

et al. 2018

IJMS

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Benign prostate hyperplasia (BPH) is one of the most common urological problems in mid-aged to elderly men. Risk factors of BPH include family history, obesity, type 2 diabetes, and high oxidative stress. The main medication classes for BPH management are alpha blockers and 5α-reductase inhibitors. However, these conventional medicines cause adverse effects. Lycogen™, extracted from Rhodobacter sphaeroides WL-APD911, is an anti-oxidant and anti-inflammatory compound. In this study, the effect of Lycogen™ was evaluated in rats with testosterone-induced benign prostate hyperplasia (BPH). Testosterone injections and Lycogen™ administration were carried out for 28 days, and body weights were recorded twice per week. The testosterone injection successfully induced a prostate enlargement. BPH-induced rats treated with different doses of Lycogen™ exhibited a significantly decreased prostate index (PI). Moreover, the Lycogen™ administration recovered the histological abnormalities observed in the prostate of BPH rats. In conclusion, these findings support a dose-dependent preventing effect of Lycogen™ on testosterone-induced BPH in rats and suggest that Lycogen™ may be favorable to the prevention and management of benign prostate hyperplasia.

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“…Generation of reactive oxygen species (ROS) and ischemia - reperfusion injury have been proposed as the primary etiological factors in obstruction - induced bladder dysfunction (9). At a molecular level, reactive oxygen species exhibit signaling and cell - function - modifying roles (27).…”

Section: Discussionmentioning

confidence: 99%

“…OS induces lipid peroxidation, which is expressed by formation of malondialdehyde (MDA) (8). Sezginer et al has recently investigated the effects of different degrees of obstruction on bladder function in rats, showing that MDA levels were increased in severe partial BOO (9). Nevertheless, this association has not been properly assessed in humans so far.…”

Section: Introductionmentioning

confidence: 99%

Oxidative stress in the bladder of men with LUTS undergoing open prostatectomy: a pilot study

et al. 2018

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Purpose: This study aims to evaluate the link between preoperative parameters and oxidative stress (OS) markers in the bladder wall of men undergoing open prostatectomy. Materials and Methods: From July 2014 to August 2016, men aged ≥ 50 years and presenting with LUTS were prospectively enrolled. Preoperative assessment included validated questionnaires (IPSS and OAB - V8), lower urinary tract ultrasound and urodynamics. Bladder biopsies were taken during open prostatectomy for determination of OS markers. Increased OS was defined by increased concentration of malondialdehyde (MDA) and / or decreased concentration of antioxidant enzymes (superoxide dismutase and / or catalase). P<0.05 was regarded as statistically significant. Results: Thirty - eight consecutive patients were included. Mean age was 66.36 ± 6.44 years, mean prostate volume was 77.7 ± 20.63 cm3, and mean IPSS was 11.05 ± 8.72 points. MDA concentration was increased in men with severe bladder outlet obstruction (BOO grade V - VI according to the Schaefer's nomogram) in comparison with BOO grade III - IV (p = 0.022). Patients with severe LUTS also had higher MDA concentration when compared to those with mild LUTS (p = 0.031). There was a statistically significant association between increased post - void residual urine (cut off ≥ 50 mL) and not only higher levels of MDA, but also reduced activity of SOD and catalase (p < 0.05). Conclusions: This pilot study showed that severity of LUTS and BOO were associated with increased MDA concentration in the bladder wall of men undergoing open prostatectomy. Further studies are still needed to assess the role of non - invasive biomarkers of OS in predicting bladder dysfunction in men with LUTS.

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Effects of varying degrees of partial bladder outlet obstruction on urinary bladder function of rats: A novel link to inflammation, oxidative stress and hypoxia

Cited by 22 publications

References 47 publications

A Review of Oxidative Stress and Urinary Dysfunction Caused by Bladder Outlet Obstruction and Treatments Using Antioxidants

A Review of Oxidative Stress and Urinary Dysfunction Caused by Bladder Outlet Obstruction and Treatments Using Antioxidants

Rhodobacter sphaeroides Extract Lycogen™ Attenuates Testosterone-Induced Benign Prostate Hyperplasia in Rats

Oxidative stress in the bladder of men with LUTS undergoing open prostatectomy: a pilot study

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