Effects of vasopressin on gene expression in rat inner ear

Gu, Fei; Han, Honglei; Zhang, Lianshan

doi:10.1016/j.heares.2006.08.016

Cited by 25 publications

(12 citation statements)

References 36 publications

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“…However, data from Patil et al (1997) suggested that AQP1 may also be regulated by AVP in Xenopus oocytes. More recently AQP7 was found decreased in response to AVP (Gu et al, 2006). AVP has an effect on water metabolism of the amniotic membranes (Ervin et al, 1986).…”

Section: Introductionmentioning

confidence: 95%

AQP1 gene expression is upregulated by arginine vasopressin and cyclic AMP agonists in trophoblast cells

et al. 2008

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Section: Introductionmentioning

confidence: 95%

AQP1 gene expression is upregulated by arginine vasopressin and cyclic AMP agonists in trophoblast cells

et al. 2008

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“…While it has been shown that aquaporin 3 mRNA expression is upregulated by intra-endolymphatic sac application of dexamethasone 11 , little is known of the direct molecular impact on other ion homeostasis genes by therapeutic glucocorticoids delivered trans-tympanically, a common approach to treating hearing disorders. Ion homeostasis genes are active in the inner ear not only in maintaining the ionic microenvironment required for hearing, but also in response to vasopressin 12 and other molecules 13 .…”

Section: Introductionmentioning

confidence: 99%

Intratympanic Steroid Treatments May Improve Hearing via Ion Homeostasis Alterations and Not Immune Suppression

MacArthur¹,

Hausman²,

Kempton³

et al. 2015

Otology &Amp; Neurotology

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Hypothesis The inner ear endothelium is capable of responding to therapeutic steroids by altering local expression of cytokine and ion homeostasis genes that impact inflammation and fluid regulation. Background Glucocorticoids are often given trans-tympanically for hearing disorders because of their anti-inflammatory effects, but their direct impact on inner ear ion homeostasis and cytokine gene expression has not been studied. Methods The middle ears of Balb/c mice were transtympanically injected with 5 µl of either phosphate-buffered saline (PBS), prednisolone or dexamethasone. Untreated mice were used as controls. Mice were euthanized at 6, 24 and 72 hours, the cochleas harvested and total RNA isolated from the inner ear tissues. Expression of eight cytokine genes and 24 ion homeostasis genes were analyzed with qRT-PCR. Results PBS caused upregulation of inflammatory cytokine genes that peaked at 6 hours. Surprisingly, prednisolone and dexamethasone also caused upregulation of most cytokine genes. Interestingly, ion homeostasis genes were predominantly upregulated with dexamethasone and prednisolone, with prednisolone having the larger effect. Conclusion In the murine model, intratympanic steroids caused an initial upregulation of inflammatory cytokine genes in the inner ear, as well as predominantly upregulation of ion homeostasis genes. These findings suggest glucorticoids do not suppress inner ear inflammation, but rather cause an initial inflammatory response in the inner ear. Thus, inflammatory gene suppression is not a likely mechanism for their hearing restorative effects. On the other hand, these steroids have a significant mineralocorticoid function, as demonstrated by increased function of ion homeostasis genes, implicating their ionic and fluid regulatory properties as a mechanism for their therapeutic effects.

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“…The etiology of endolymphatic hydrops and Meniere's disease, common causes of episodic vertigo and fluctuating hearing loss in humans, are poorly understood. Rats injected with vasopressin, as a model for Meniere's disease, were found to have an upregulation of AQP2 and a downregulation of AQP7 mRNAs (Gu et al, 2006). Finally, Hawkins et al performed a wide scale analysis of the gene expression profiles of cultured mature chick cochleae and utricles that were exposed either to neomycin treatment or laser ablation, in order to identify transcription factors and signaling pathways essential for hair cell regeneration (Hawkins et al, 2007).…”

Section: Gene Expression Analysis In Response To Aging Injury or Othmentioning

confidence: 99%

High throughput gene expression analysis of the inner ear

Hertzano

Elkon

2012

Hearing Research

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Effects of vasopressin on gene expression in rat inner ear

Cited by 25 publications

References 36 publications

AQP1 gene expression is upregulated by arginine vasopressin and cyclic AMP agonists in trophoblast cells

AQP1 gene expression is upregulated by arginine vasopressin and cyclic AMP agonists in trophoblast cells

Intratympanic Steroid Treatments May Improve Hearing via Ion Homeostasis Alterations and Not Immune Suppression

High throughput gene expression analysis of the inner ear

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