Effects of Viola yedoensis Makino anti-itching compound on degranulation and cytokine generation in RBL-2H3 mast cells

Zeng, Hairong; Bing, Wang; Zhao, Zhen Z.; Zhang, Qi; Liang, Mei-Yun; Yao, Ya-Qi; Bian, Ka; Zhang, Weirong

doi:10.1016/j.jep.2016.05.030

Cited by 21 publications

(9 citation statements)

References 38 publications

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“…Mast cells contain a large amount of cytoplasmic granules, which activation caused the process of degranulation (Zeng et al, 2016). The release of β-hexosaminidase can be used to quantify the extent of degranulation (Huang et al, 2015).…”

Section: Resultsmentioning

confidence: 99%

“…Degradation of granules in mast cells leads to the release of the enzyme β-hexosaminidase along with histamine. Therefore, β-hexosaminidase is often used as a marker to measure the efficacy of new drugs in preventing mast cell activation and degranulation (Huang et al, 2014; Zeng et al, 2016). Using this assay, we confirmed that ZCO significantly inhibited anti-DNP IgE-mediated and PMA/A23187-stimulated β-hexosaminidase release (Figure 2).…”

Section: Discussionmentioning

confidence: 99%

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Anti-allergic Inflammatory Effects of the Essential Oil From Fruits of Zanthoxylum coreanum Nakai

Guo

Park

et al. 2018

Front. Pharmacol.

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Zanthoxylum coreanum Nakai is a rare shrub which grows in Korea and China. Pericarp of Z. coreanum has been used as a crude medicine, but there are few researches about the pharmacologic activities. The present study was designed to investigate the anti-allergic inflammatory activities of the essential oil from fruits of Zanthoxylum coreanum Nakai (ZCO). Our findings showed that ZCO inhibited both the IgE-antigen complex or PMA/A23187-induced β-hexosaminidase release and IL-4 production dose-dependently in RBL-2H3 mast cells, and confirmed that ZCO at the tested concentrations did not show cytotoxicity to RBL-2H3 cells by MTS assay. Additionally, we found that ZCO showed the significant inhibition on LPS-induced overproduction of TNF-α, IL-6 and NO. Consistently, the protein levels of iNOS and COX-2 were also remarkably decreased by ZCO treatment. Herein, Our mechanistic studies revealed that ZCO significantly suppressed the activation of transcription factor NF-κB in PMA-activated 293T cells, and further inhibited NF-κB p65 translocation into the nucleus in LPS-stimulated RAW264.7 cells. Further investigation identified that ZCO down-regulated LPS-induced phosphorylation of MAPK (JNK, ERK, and p38) signal pathway. For incremental research, we established an DNCB-induced atopic dermatitis model in BALB/c mice, and found that ZCO remarkably inhibited DNCB-induced ear swelling and AD-like symptoms. Based on these findings, ZCO is suggested to have a therapeutic potential for the allergic inflammatory diseases.

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Section: Resultsmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Anti-allergic Inflammatory Effects of the Essential Oil From Fruits of Zanthoxylum coreanum Nakai

Guo

Park

et al. 2018

Front. Pharmacol.

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“…Viola yedoensis Makino, was widely distributed in China, especially in Sichuan Province, [11] which was used to treat inflammation [12,13] and viral hepatitis [14] . Its taste is bitter, spicy, and cold, and has the effects of clearing heat and removing toxicity, cooling blood and detumescence [15] .…”

Section: Introductionmentioning

confidence: 99%

“…Its taste is bitter, spicy, and cold, and has the effects of clearing heat and removing toxicity, cooling blood and detumescence [15] . It was used as an antifebrile and detoxicant agent for the treatment of inflammatory diseases such as boils, furuncles, carbuncles, acute and chronic hepatitis, and other infections [16] . The theory of TCM believed that the main pathogenesis of liver injury were summer heat dampness, hot, phlegm, toxin, and consumptive disease, and it was the deficiency of the spleen and kidney [17] .…”

Section: Introductionmentioning

confidence: 99%

Protective Effect of n‐Butanol Extract from Viola yedoensis on Immunological Liver Injury

Chu

Wang

et al. 2021

Chemistry & Biodiversity

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Viola yedoensis Makino was used to treat inflammation, viral hepatitis, acute pyogenic infection, and ulcerative carbuncles. However, the protective effect on immunological liver injury (ILI) of V. yedoensis had been rarely reported. This study aimed to explore the protective effect of n‐butanol extract (BE) from V. yedoensis on ILI in vitro and in vivo. In vitro, the BE significantly inhibited the secretions of Hepatitis B surface antigen (HBsAg) and Hepatitis B e antigen (HBeAg) in the HepG2.2.15 cells and the replication of HBV DNA. The research data in vivo revealed that the BE reduced the levels of alanine transaminase (ALT), aspartate transaminase (AST), and methane dicarboxylic aldehyde (MDA) in liver tissues of the ConA‐induced mice, while increased the activities of superoxide dismutase (SOD), glutathione peroxidase (GSH‐PX), and the effective contents of tumor necrosis factor‐α (TNF‐α), interferon‐γ (IFN‐γ) and the BE could ameliorate liver histological lesions. These results motivated a further investigation into the chemical constituents of BE. Four coumarins (esculetin, prionanthoside, cichoriin, and esculin) and one flavonoid (quercetin‐3‐O‐galactoside) were isolated from the BE by silica gel column chromatography and recrystallization, of which structures were eventually confirmed by 1H‐NMR, 13C‐NMR, and MS.

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“…Earlier reports have indicated that V. yedoensis contains flavone (including mono-C-hexoside, 6,8-di-C-hexosides, 6,8-di-C-pentosides, 6,8-C-hexosyl-C-pentosides, C-glycosides, and O-glycosides), dicoumarin (including dimeresculetin, euphorbetin, and esculetin) and cyclotides 10 , 13 , 14 . V. yedoensis inhibits β-hexosaminidase and histamine release and down-regulates the expression of inflammatory cytokines (such as IL-1β, TNF-α, IL-6, and iNOS) to block the inflammatory development in RBL-2H3 mast cells 15 . Nevertheless, the anti-cancer effect of V. yedoensis in human lung adenocarcinoma has not been investigated.…”

Section: Introductionmentioning

confidence: 99%

Viola Yedoensis Suppresses Cell Invasion by Targeting the Protease and NF-κB Activities in A549 and Lewis Lung Carcinoma Cells

et al. 2018

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Cancer metastasis is a vital trait in malignancies with complicated early diagnosis and therapeutic management. Therefore, the development of new remedies and the utilization of natural medicines that target metastasis are of great interest and have been studied extensively. Chinese medicinal herbs have various anti-carcinogenesis properties; however, the in vitro effect and mechanism of Viola yedoensis on cancer cell metastasis remains poorly understood. V. yedoensis extracts (VYE) can suppress the invasion of a highly metastatic human lung cancer cell line, A549 cells. According to gelatin zymography and casein zymography assays, VYE inhibited the activities of matrix metalloproteinases (MMPs) and urokinase-type plasminogen activator (u-PA). The results of reverse transcription-polymerase chain reaction and Western blotting revealed that VYE can alter the expression of proteinase inhibitor. VYE also suppressed the DNA binding activity of nuclear factor-kappa B. We concluded that VYE may inhibit tumor invasion by suppressing the activities of MMP and u-PA in lung cancer cells.

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Effects of Viola yedoensis Makino anti-itching compound on degranulation and cytokine generation in RBL-2H3 mast cells

Cited by 21 publications

References 38 publications

Anti-allergic Inflammatory Effects of the Essential Oil From Fruits of Zanthoxylum coreanum Nakai

Anti-allergic Inflammatory Effects of the Essential Oil From Fruits of Zanthoxylum coreanum Nakai

Protective Effect of n‐Butanol Extract from Viola yedoensis on Immunological Liver Injury

Viola Yedoensis Suppresses Cell Invasion by Targeting the Protease and NF-κB Activities in A549 and Lewis Lung Carcinoma Cells

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