Effects of vitamin A on doxorubicin-induced chromosomal aberrations in bone marrow cells of rats

Gülkaç, Mehmet Doğan

doi:10.1093/mutage/geh021

Cited by 38 publications

(12 citation statements)

References 41 publications

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“…Also, DOX caused high percentage of structural and numerical chromosome aberrations. These results are similar to the previous studies which provided evidence that DOX induced hepatotoxicity and chromosomal aberrations [29][30][31]. On the other hand, Herbs are rich in various compounds such triglycerides, flavonoids, and polyphenols, that can defend against damages induced by toxic drugs [32].…”

Section: Discussionsupporting

confidence: 91%

Protective Effect of Artemisia judaica against Doxorubicin-Induced Toxicity in Mice

Ahmed

Mabrouk

Hassanane

et al. 2017

ARRB

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Aim: The present study aimed to investigate the protective effect of Artemisia Judaica (A. Judaica) against doxorubicin (DOX)-induced toxicity in male mice. Place and Duration of Study: Department of Cell Biology, Genetic engineering and biotechnology division, National research centre, Egypt, between March 2016 and February 2017. Methodology: Male mice were divided into 7 groups (n=10) and treated as follow: the control group, the group treated with DMSO, the group injected (i.p.) with DOX , the groups treated with low and high dose of A. judaica extract and the groups injected (i.p.) with DOX and treated with low and high dose of A. judaica extract. Femur, testes and liver samples were collected for different analyses. Results: Our data showed that A. judaica significantly reversed the DOX-induced elevation of DNA fragmentation rate and MDA level in liver tissue, as well as declined chromosomal aberrations (CAs) either in the bone marrow cells or in the spermatocyte cells. Meanwhile, the expression of Ahmed et al.; ARRB, 18(3): 1-10, 2017; Article no.ARRB.35990 2 apoptosis-related genes (Bax and Caspase-3) in liver tissues was analyzed by quantitative real-time PCR (qRT-PCR) and results revealed that genes expression were up-regulated in DOX treated mice however; the administration of A. judaica didn't alter such increase. Conclusion: Overall, the findings indicated that A. judaica may attenuate the DOX-induced toxicity. However; further studies are required to confirm the protective effect of A. judaica extract against toxicity caused by DOX drug. Original Research Article

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Section: Discussionsupporting

confidence: 91%

Protective Effect of Artemisia judaica against Doxorubicin-Induced Toxicity in Mice

Ahmed

Mabrouk

Hassanane

et al. 2017

ARRB

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“…Similar results have also been observed in studies on other mammalian groups, which reported higher incidences of chromatid-type CAs (Newsome and Littlefield, 1975; Antunes and Takahashi, 1998; Gülkaç et al , 2004). High frequencies of chromatid damage are justified by the fact that the lymphocytes were exposed to a mutagenic agent during the S or G 2 phases, when the chromosomes have two sister chromatids (Evans and O'Riordan, 1975).…”

Section: Discussionsupporting

confidence: 90%

Identifying Mazama gouazoubira (Artiodactyla; Cervidae) chromosomes involved in rearrangements induced by doxorubicin

2017

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The process of karyotype evolution in Cervidae from a common ancestor (2n = 70, FN = 70) has been marked by complex chromosomal rearrangements. This ancestral karyotype has been retained by the current species Mazama gouazoubira (Fischer 1814), for which a chromosomal polymorphism (Robertsonian translocations and the presence of B chromosomes) has been described, presumably caused by a chromosome fragility. Thus, this study has identified doxorubicin-induced chromosome aberrations and mapped the regions involved in breaks, which may be related to the chromosome evolution process. G-banding pattern showed that 21 pairs of chromosomes presented chromosomal aberrations, 60% of the total chromosome number of the species M. gouazoubira. Among chromosomes that carry aberrations, the region where they were most frequently concentrated was distal relative to the centromere. These data suggest that certain chromosomal regions may be more susceptible to chromosome fragility and consequently could be involved in karyotype differentiation in species of the family Cervidae.

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“…The pellets were then fixed using methanol: acetic acid (3:1, v/v) fixative and stained with Giemsa stain. One hundred well‐spread metaphase cells were scored for each animal and structural chromosomal aberrations were observed and recorded [28].…”

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confidence: 99%

Anti‐Clastogenic Effect of Berberine against DMBA‐Induced Clastogenesis

Sindhu

Manoharan

2010

Basic Clin Pharma Tox

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Our aim was to evaluate the protective effect of berberine on 7,12-dimethylbenz [a]anthracene (DMBA)-induced chromosomal aberrations and micro-nucleated polychromatic erythrocytes (MnPCEs) frequency in bone marrow cells of golden Syrian hamsters. The anti-clastogenic effect of berberine (50 mg ⁄ kg b.w. p.o.) was also assessed by measuring the status of phase II detoxification enzymes and oxidative stress, as biochemical endpoints, during DMBA (30 mg ⁄ kg b.w. i.p.) induced clastogenesis. Marked chromosomal aberrations, increased MnPCEs frequency and enhanced status of lipid peroxidation, antioxidants and phase I and II detoxification enzymes were noticed in hamsters treated with DMBA alone. Oral pretreatment with berberine for 5 days to DMBA-treated hamsters significantly reduced the frequency of MnPCEs and chromosomal abnormalities as well as reversed the status of lipid peroxidation, antioxidants and phase I and II detoxification enzymes. The present study thus suggests that berberine has potent anti-clastogenic potential against DMBA-induced clastogenesis, which is probably due to its anti-lipid peroxidative potential and effect on modulation of phase I and II detoxification cascade.Somatic cell mutation plays a crucial role in the initiation and promotion of carcinogenesis. Polycyclic aromatic hydrocarbons exert mutagenic and carcinogenic effects in biological systems if they are metabolically activated to more reactive forms [1]. 7,12-Dimethylbenz[a]anthracene (DMBA), a potent and best characterised organ-specific carcinogen, attacks DNA through its active metabolite 4-dihydrodiol-1,2-epoxide. DMBA firmly binds to epidermal DNA and generates a complex DNA adduct profile [2]. DMBA is therefore widely used to induce clastogenesis in experimental animals.The investigation of new anti-clastogenic and anti-carcinogenic compounds is essential in the modern human civilization due to rise in undesirable genetic mutations and increase in cancer risk [3]. In vivo models with laboratory animals are the reliable approaches to predict the clastogenic or anti-clastogenic potential of natural or synthetic agents [4]. Bone marrow cells were commonly employed as a tool for the evaluation of clastogenic and anti-clastogenic efficacy of carcinogens and anti-cancer compounds, respectively. An abnormality in chromosomal structure (translocation, deletion, duplication) and chromosomal number due to exposure to chemical carcinogen and ⁄ or mutagen is associated with increased risk of cancer and in the progression of neoplastic transformation [5]. Gaizev et al. and Hayashi et al. reported that measurement of micronuclei in polychromatic erythrocytes is suitable to identify a mutagenic substance due to the reason that their macronucleus is extruded in the processes of their formation from erythroblasts [6,7]. Also, it is easy to examine chromosomal aberrations in the metaphase stage due to its compact arrangement [8].Though a battery of genotoxicity tests is used to evaluate the anti-clastogenic effect of medicinal plant...

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Effects of vitamin A on doxorubicin-induced chromosomal aberrations in bone marrow cells of rats

Cited by 38 publications

References 41 publications

Protective Effect of Artemisia judaica against Doxorubicin-Induced Toxicity in Mice

Protective Effect of Artemisia judaica against Doxorubicin-Induced Toxicity in Mice

Identifying Mazama gouazoubira (Artiodactyla; Cervidae) chromosomes involved in rearrangements induced by doxorubicin

Anti‐Clastogenic Effect of Berberine against DMBA‐Induced Clastogenesis

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