Effects of Vitamin C Supplementation on the Chronic Phase of Chagas Disease

Marim, Ricardo Guimarães; Gusmão, Alex Silva de; Castanho, Roberto Esteves Pires; Deminice, Rafael; Therezo, Altino Luiz Silva; Júnior, Alceu Afonso Jordão; Assis, Marcos Renato de; Taipeiro, Elane de Fátima; Martins, Luciamáre Perinetti Alves

doi:10.1590/s0036-46652015000300011

Cited by 14 publications

(9 citation statements)

References 37 publications

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“…The use of nutritional supplements to aid in host response against protozoan parasites has been studied, some of which revealed anti-trypanosome effects. Ascorbic acid (vitamin C) has been shown to aid in combating the oxidative stress injuries in vital organs of mice infected with Trypanosoma cruzi [ 9 ]. Epigallocatechin gallate (an analog of green tea extract) administration to mice showed significant levels of decreased parasitism and increased survival rates suggesting that epigallocatechin gallate may be potentially useful for the protection against T. cruzi [ 10 ].…”

Section: Introductionmentioning

confidence: 99%

Anti-trypanosome effects of nutritional supplements and vitamin D3: in vitro and in vivo efficacy against Trypanosoma brucei brucei

et al. 2016

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BackgroundPrevious publications suggest that nutritional supplements have anti-trypanosome activity in vitro, although apparent efficacy was not noted in vivo. This study was conducted by experimentally infecting mice with Trypanosoma brucei brucei to assess the anti-trypanosome activity of various nutritional supplements with the hope of finding possible application in the treatment of African trypanosomiasis.MethodsActivities of nutritional supplements were screened in vitro against bloodstream forms of T. b. brucei. To evaluate selectivity, we used two mammalian cells, Jurkat cells and Vero cells. The IC50 values and selectivity index values were calculated, and supplements with promising efficacy in vitro were selected for further testing in vivo. Mice were infected intraperitoneally with 1 × 103T. b. brucei. We observed parameters for disease progression such as parasitemia, red blood cell count, white blood cell count, survivability, and splenomegaly. Morphological profiles after the treatment were analyzed by scanning electron microscopy.ResultsVitamin D3 showed anti-trypanosome efficacies both in vitro and in vivo. It seemed to have suppressive effects on parasitemia, and spleen weight was also significantly lower in vitamin D3-treated mice when compared to non-treated control mice. There was, however, no significant prolonged survivability of infected mice treated with vitamin D3. Among green tea extracts, polyphenon-60 and epigallocatechin gallate had suppressive effects against T. b. brucei in vitro, but in vivo efficacies were marginal.ConclusionsTreatment with nutritional supplements, vitamin D3, and polyphenon-60 seemed to have anti-trypanosome activity in vitro and protective activity to some extent in vivo, respectively, although those supplements themselves did not have curable effects. The exact mechanisms of action are not clear, but the significant efficacy in vitro suggested direct effects of supplements against African trypanosome parasites.

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Section: Introductionmentioning

confidence: 99%

Anti-trypanosome effects of nutritional supplements and vitamin D3: in vitro and in vivo efficacy against Trypanosoma brucei brucei

et al. 2016

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“…In light of this finding, it may be postulated that in our study due to intoxication with high dose of ParCM in group D, the activity of heme oxygenase might have accelerated resulting in frequent availability of free transition iron and presence of AsAc, causing reduction of transition metal ions, boost of the Fenton reaction, and detrimental effects on the liver cells. Wang et al (2007), Park and Lee (2008), Kamel et al (2010), and Marim et al (2015) have all reported the damaging effects of AsAc in their studies (Kamel et al, 2010;Marim et al, 2015;Park and Lee, 2008;Wang et al, 2007).…”

Section: Discussionmentioning

confidence: 98%

Hepatoprotective effect of a polyherbal formulation and ascorbic acid in paracetamol induced hepatic damage in rabbits

Fiaz

Shakir

et al. 2017

Biomed. Res. Ther.

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Background: The hepatoprotective effect of a polyherbal formulation was evaluated in female rabbits. The herbal formulation was used alone and in combination with ascorbic acid (AsAc) in animals with paracetamol (ParCM)-induced hepatic damage. Methods: The study design included five groups, each comprised of five animals. Group A was the control group (untreated; given only routine diet) while group B was given a single oral dose of ParCM (2 g/kg) on day 9. Groups C, D and E were pretreated with polyherbal formulation (PoHF; 500 mg/kg), ascorbic acid (AsAc; 200 mg/kg) and PoHF (500 mg/kg) combined with ascorbic acid (AsAc; 200 mg/kg), respectively for 9 consecutive days. On the last day (day 9), after 30 minutes of routine treatments, a single dose of ParCM (2 g/ kg) was administered in groups C, D and E. Animals were sacrificed 24 hours after the last treatment. Blood and liver samples were collected from all animals. Serum was separated from the blood samples and subjected to biochemical tests for liver biomarker analysis. The biomarkers included alanine aminotransferase (ALT), aspartate aminotransferase (AST), alkaline phosphatase (ALP) and total bilirubin. Elevation of enzyme markers was considered an indicator of hepatocellular injury. Results: Serum levels of liver enzymes and total bilirubin were elevated significantly in group B when compared to group A. The level of serobiochemicals significantly dropped in group C but increased significantly in group D, as compared to group B.

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“…in Chagas mice, though this seemed to be a dose-dependent effect; treatment with 60 mg vitC was not oxidative, while treatment with 500 mg of vitC per day led to increased total peroxide and TBARS at 60 and 180 days [52,60].…”

Section: Oxidative Medicine and Cellular Longevitymentioning

confidence: 90%

Potential Role of Antioxidants as Adjunctive Therapy in Chagas Disease

Sánchez-Villamil

Bautista-Niño

Serrano

et al. 2020

Oxidative Medicine and Cellular Longevity

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Chagas disease (CD) is one of the most important neglected tropical diseases in the American continent. Host-derived nitroxidative stress in response to Trypanosoma cruzi infection can induce tissue damage contributing to the progression of Chagas disease. Antioxidant supplementation has been suggested as adjuvant therapy to current treatment. In this article, we synthesize and discuss the current evidence regarding the use of antioxidants as adjunctive compounds to fight harmful reactive oxygen species and lower the tissue oxidative damage during progression of chronic Chagas disease. Several antioxidants evaluated in recent studies have shown potential benefits for the control of oxidative stress in the host’s tissues. Melatonin, resveratrol, the combination of vitamin C/vitamin E (vitC/vitE) or curcumin/benznidazole, and mitochondria-targeted antioxidants seem to be beneficial in reducing plasma and cardiac levels of lipid peroxidation products. Nevertheless, further research is needed to validate beneficial effects of antioxidant therapies in Chagas disease.

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Effects of Vitamin C Supplementation on the Chronic Phase of Chagas Disease

Cited by 14 publications

References 37 publications

Anti-trypanosome effects of nutritional supplements and vitamin D3: in vitro and in vivo efficacy against Trypanosoma brucei brucei

Anti-trypanosome effects of nutritional supplements and vitamin D3: in vitro and in vivo efficacy against Trypanosoma brucei brucei

Hepatoprotective effect of a polyherbal formulation and ascorbic acid in paracetamol induced hepatic damage in rabbits

Potential Role of Antioxidants as Adjunctive Therapy in Chagas Disease

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