Effects of Vitamin D Deficiency on Proliferation and Autophagy of Ovarian and Liver Tissues in a Rat Model of Polycystic Ovary Syndrome

Lajtai, Krisztina; Nagy, Csilla Terézia; Tarszabó, Róbert; Benkó, Rita; Hadjadj, Leila; Sziva, Réka Eszter; Gerszi, Dóra; Bányai, Bálint; Ferdinándy, Péter; Nádasy, György L.; Giricz, Zoltán; Horváth, Eszter; Várbı́ró, Szabolcs

doi:10.3390/biom9090471

Cited by 20 publications

(12 citation statements)

References 48 publications

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“…Testosterone levels of the animals following treatment has been published by Hadjadj et al, respectively: T-D+: 0.311 ± 0.16, T + D+: 4.292 ± 0.56, T-D-: 0.720 ± 0.16, T + D-: 5.495 ± 0.56 (ng/ml, mean ± SEM) [ 10 ]. Testosterone-treated groups had no estrus cycles [ 11 ]. PCO morphology was also a characteristic of testosterone treatment groups, and representative images of the corresponding ovaries were published by Pal et al [ 12 ].…”

Section: Discussionmentioning

confidence: 99%

Effect of Vitamin D Status on Vascular Function of the Aorta in a Rat Model of PCOS

Lajtai

Tarszabó

Bányai³

et al. 2021

Oxidative Medicine and Cellular Longevity

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Polycystic ovary syndrome (PCOS) is associated with elevated cardiovascular risk. Early vascular dysfunction may lead to the development of cardiovascular disease in PCOS. Vitamin D deficiency (VDD) is a common comorbidity of PCOS that contributes to the pathogenesis of the disease and its complications. Both PCOS and VDD are accompanied by increased oxidative stress that may be involved in the arising vascular dysfunction. We aimed to investigate the role of vitamin D status on aortic function. PCOS was induced by an 8-week-long transdermal testosterone treatment of female rats, and low and adequate vitamin D status was achieved by dietary means. Contraction and relaxation abilities of isolated aortic segments were measured by myograph. Resorcin-fuchsin staining and immunohistochemical labeling of 3-nitrotyrosine were performed. No difference was shown in the norepinephrine-induced contraction of the aortas of different groups, whereas we detected reduced acetylcholine- and insulin-evoked relaxation in VDD groups. A lower level of resorcin-fuchsin staining and elevated 3-nitrotyrosine immunostaining was observed in VDD. In our study, we demonstrated early endothelial dysfunction in VDD PCOS rat model. Vitamin D supplementation could prevent vascular disturbances, while VDD itself damaged endothelium-dependent vasorelaxation and induced nitrative stress.

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Section: Discussionmentioning

confidence: 99%

Effect of Vitamin D Status on Vascular Function of the Aorta in a Rat Model of PCOS

Lajtai

Tarszabó

Bányai³

et al. 2021

Oxidative Medicine and Cellular Longevity

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“…Low (<100 IU/kg) vitamin D dietary intake caused significant increase in the level of 3-nitrotyrosine in the male rat brain [ 48 ]. Vitamin-D-deficient female rats showed elevated NT expression in liver and ovaries [ 49 ]. Accumulation of HNE-modified macromolecules significantly influences several cell functions, interactions, and metabolism: DNA, RNA, protein synthesis inhibition, mitochondrial dysfunction, protein aggregation, cell cycle arrest, cell death, and impaired DNA-repair mechanisms have been found [ 50 ].…”

Section: Discussion Limitations and Strengthsmentioning

confidence: 99%

Vitamin D Deficiency Induces Elevated Oxidative and Biomechanical Damage in Coronary Arterioles in Male Rats

et al. 2020

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Background: Several reports prove interconnection between vitamin D (VD) deficiency and increased cardiovascular risk. Our aim was to investigate the effects of VD status on biomechanical and oxidative–nitrative (O–N) stress parameters of coronary arterioles in rats. Methods: 4-week-old male Wistar rats were divided into a control group (11 animals) with optimal VD supply (300 IU/kgbw/day) and a VD-deficient group (11 animals, <5 IU/kg/day). After 8 weeks, coronary arteriole segments were prepared. Geometrical, elastic, and biomechanical characteristics were measured by in vitro arteriography. O–N stress markers were investigated by immunohistochemistry. Results: Inner radius decreased; wall thickness and wall-thickness/lumen diameter ratio increased; tangential wall stress and elastic modulus were reduced in VD-deficient group. No difference could be found in wall-cross-sectional area, intima-media area %. While the elastic elements of the vessel wall decreased, the α-smooth muscle actin (α-SMA) immunostaining intensity showed no changes. Significant elevation was found in the lipid peroxidation marker of 4-hidroxy-2-nonenal (HNE), while other O–N stress markers staining intensity (poly(ADP)ribose, 3-nitrotyrosine) did not change. Conclusions: Inward eutrophic remodeling has developed. The potential background of these impairments may involve the initial change in oxidative damage markers (HNE). These mechanisms can contribute to the increased incidence of the cardiovascular diseases in VD deficiency.

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“…However, the exact molecular mechanisms of the effects of vitamin D on the improvement of IR and PCOS are not yet clear [103]. The possible role for folate and vitamin B 12 is decreasing the serum levels of homocysteine among PCOS women with IR [104,105] and also for zinc is modifying the clinical and in ammatory biomarkers of PCOS women [11].…”

Section: Discussionmentioning

confidence: 99%

Association Between the Food Security Status and Dietary Patterns With Polycystic Ovary Syndrome (PCOS) in Overweight and Obese Iranian Women: A Case-Control Study

Badri-Fariman

Naeini

Mirzaei

et al. 2021

Preprint

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Background Polycystic ovary syndrome (PCOS) as one of the major types of endocrine disorders is common among women worldwide. Frequently reported that food insecurity (FI) and unhealthy diets can exert negative effects on reproductive health. There are contradictory results on optimal lifestyle modification especially dietary components with PCOS. This paper aimed to assess the association between PCOS with food security status and dietary patterns of overweight and obese Iranian women. Methods This case-control study was performed on 240 overweight and obese adult women with PCOS (n = 120) and without PCOS (n = 120) referred to the infertility clinic of Arash Hospital in Tehran, Iran. Participants' data on anthropometry, scio-economic factors, and physical activity were collected. Food security status and dietary intakes were assessed by validated questionnaires. Results The prevalence of FI was 60% in women with PCOS and 30% in healthy women. There was a positive association between the risk of PCOS with FI, quasi-western dietary pattern, low economic level, waist circumference, and menstrual age and a negative association between physical activity and healthy dietary pattern with this risk even after controlling the potential confounders (P < 0.05). PCOS women had a higher intake of saturated fats, monounsaturated fats, oleic acid, fluorine, sucrose, and caffeine and a lower intake of vitamins A, B5, B6, B12, C, and D, potassium, proteins, carbohydrates, cholesterols, docosahexaenoic acid, potassium, carotenes, lutein, beta-cryptoxanthin, lycopene, calcium, iron, thiamine, riboflavin, niacin, tetra- and dihydrofolate, biotin, phosphorus, magnesium, zinc, copper, total, insoluble, and crude fiber, glucose, galactose, fructose, and lactose compared to the healthy group (P < 0.05). Conclusions FI, quasi-western dietary pattern, low economic status, and waist circumference were significantly associated with the higher risk of PCOS. Modifying lifestyle especially in dietary patterns may be an important strategy for the reduction of PCOS. Future prospective studies are warranted to confirm these findings and to identify the underlying mechanisms.

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Effects of Vitamin D Deficiency on Proliferation and Autophagy of Ovarian and Liver Tissues in a Rat Model of Polycystic Ovary Syndrome

Cited by 20 publications

References 48 publications

Effect of Vitamin D Status on Vascular Function of the Aorta in a Rat Model of PCOS

Effect of Vitamin D Status on Vascular Function of the Aorta in a Rat Model of PCOS

Vitamin D Deficiency Induces Elevated Oxidative and Biomechanical Damage in Coronary Arterioles in Male Rats

Association Between the Food Security Status and Dietary Patterns With Polycystic Ovary Syndrome (PCOS) in Overweight and Obese Iranian Women: A Case-Control Study

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