Effects of vitamin D on apoptosis of T-lymphocyte subsets in neonatal sepsis

Abstract

Effect of vitamin D on apoptosis of peripheral blood T-lymphocyte subsets in treatment of neonatal sepsis was investigated. A total of 150 neonatal patients with sepsis were randomly divided into vitamin D treatment group (observation group) and treatment control group, while 100 healthy newborns were selected as healthy control group. T-lymphocyte subsets were detected by flow cytometer, the levels of tumor necrosis factor-α, interleukin-1 and calcitonin were determined by double-antibody immunoluminometric assay, and the effect of vitamin D on the above indicators in the treatment of sepsis was observed. Serum 25(OH)D (22.52±5.56 mg/l) in the treatment group was obviously increased compared with that in the treatment group (14.85±6.14 mg/l) (P<0.05), but the levels in the two groups were remarkably lower than that in the normal control group (26.38±6.56 mg/l), and the differences were statistically significant (P<0.05). Cluster of differentiation 4 (CD4+) T-lymphocyte subset in sepsis patients was obviously reduced compared with that in the healthy control group (P<0.01); the difference in comparison of CD8+ T-lymphocyte subset between sepsis patients and healthy people was not statistically significant (P>0.05). After treatment for 72 h, CD4+ T-lymphocytes were increased, and the ratio of CD4+ to CD8+ was close to 1, suggesting that the effect was superior to that in the treatment control group. The inflammatory factor levels in children with sepsis were evidently higher than those in the healthy control group (P<0.01), and high-level states of inflammatory factors were significantly improved after treatment with vitamin D for 72 h, indicating that the effect was superior to that in the treatment group. The results indicated that the prognosis of sepsis patients treated with vitamin D is improved, and the mechanism may be achieved by regulating T-lymphocyte subsets and inflammatory factors.

Vitamin D status was associated with sepsis in critically ill children

“…[ 41 ] Vitamin D is a nutritional vitamin, but surveys have shown that children with critical illness have a high incidence of nutritional risk, and children with sepsis have a high incidence of nutritional risk of 86.4%. [ 40 ] In our meta-analysis, we demonstrated that critically ill children with sepsis might have a lower 25-OHD level than those without sepsis.…”

“…Finally, a total of 27 studies were included based on the inclusion and exclusion criteria. [ 3 , 7 – 10 , 19 – 40 ] The flowchart of the literature screening was shown in Fig. 1 .…”

“…We assessed the quality of included studies based on the NOS approach, 12 studies were regarded as high quality in case-control study, [ 7 , 19 , 21 , 23 , 24 , 27 , 28 , 30 , 34 , 38 – 40 ] and all the cohort studies were regarded as high quality with NOS >6. [ 3 , 8 , 9 , 25 , 26 , 32 , 33 , 35 – 37 ]…”

Comparison of Serum Vitamin D in the Umbilical Cord of Survived with Not Survived Premature Infants

“…Several studies have also shown the impact of vitamin D deficiency on the regulation of immune responses by creating an antimicrobial peptide pattern in epithelial cells, neutrophils, and macrophages. There are also reports of lung non-maturation through examination of the nuclear vitamin D receptor (VDR) expressed in most body tissues, which contributes to cellular regulation, diagnosis, and differentiation in premature infants (15)(16)(17)(18)(19)(20).…”

“…which can be due to maternal vitamin deficiency and causes serious problems in premature neonates, such as increased risk of respiratory problems at birth, osteopenia of prematurity, bronchopulmonary dysplasia, increased risk of infectious diseases, reduced brain growth, seizures, growth disturbances, retinopathy of immaturity, brain hemorrhage, and death (13)(14)(15). Studies report the effect of vitamin D deficiency on the regulation of immune responses by generating an antimicrobial peptide pattern in epithelial cells, neutrophils, and macrophages and the lack of lung maturation by examining the specific vitamin D receptor (VDR), which is expressed in most tissues of the body, in terms of regulation, diagnosis, and differentiation of the cell in immature died premature neonates (15)(16)(17)(18)(19)(20). Therefore, deficiency of this vitamin is one of the main challenges in the care of neonates (13).…”