Effects of Vitamin E and Vitamin C on Mercury Induced Toxicity in Mice

Abstract

The present study was undertaken to find out the efficacy of vitamin E and/or vitamin C against mercury (Hg) induced toxicity in mice. Sixty mice were randomly divided into 5 equal groups (n=12). One group of mice (Group A) was kept as control and each of rest four groups (B, C, D and E) were fed with mercuric chloride (HgCl 2 ) in drinking water @ 65 mg/L. In addition to HgCl 2 alpha-tocopherol (vitamin E) @ 100 mg/L, ascorbic acid (vitamin C) @ 250 mg/L and combination of vitamin E and vitamin C at same dose were given to the mice of groups C, D and E respectively. All treatments were continued for 28 consecutive days. Four mice of each group were sacrificed on day 1, 14 and 28 and efficacy of vitamin E and vitamin C against Hg induced toxicity were evaluated by observing toxic signs, body weight, hemato-biochemical parameters and postmortem lesions. Mild (++) toxic signs as evident by reduced feed and water intake, salivation, vomiting, excitement, muscle tremor, ataxia, restlessness, incordination and ruffled hair coat were observed from 2 nd week (group B) and from 3 rd week (group C and D) by intoxication with HgCl 2 . Significant (P<0.01) reduction of body weight (18.38%) and hematological parameters i.e. TEC (19.88%), TLC (27.89%), Hb content (34.09%) and PCV (9.15%) were observed at day 28 in HgCl 2 induced intoxicated mice (group B). At identical period in same group biochemical parameters i.e. AST (46.99%) and ALT (58.72%) increased significantly (p<0.01). Pinpoint hemorrhages throughout the liver and highly (++++) congested kidney was also observed at post mortem (group B). All the parameters i.e. toxic signs, body weight, hemato-biochemical and post mortem lesions were found to be slight (+) or mild (++) and/or improved in rest three groups of mice following treatment with vitamin E, vitamin C and combination of vitamin E and vitamin C. The present study reveals that vitamin E and C have a protective role against Hg poisoning. However, combination of vitamin E and C gave better results.

Ameliorating potency of Chenopodium album Linn. and vitamin C against mercuric chloride-induced oxidative stress in testes of Sprague Dawley rats

“…The effect of C. album is comparable with standard vitamin C group. Studies revealed that C. album contains vitamin C and E which play a vital role in hampering the absorption of Mercury in the gastrointestinal tract [11] and prevent ROS-mediated membrane damage by binding up ROS within the cell membrane [50]. It is postulated that the antioxidant CAT, SOD, and POD depletion by mercury may be a trigger for the production of reactive oxygen species (ROS) that induce lipid, protein, and DNA oxidation.…”

Estrategias dietéticas para disminuir la biodisponibilidad de mercurio desde alimentos

“…Todos estos efectos tóxicos se redujeron en presencia de vitamina C (250 mg/) o vitamina E (100 mg/L), si bien la combinación de ambas fue el tratamiento más efectivo (Huq et al, 2008). La combinación de vitamina C y ácido fólico también mostró un efecto antagonista en embriones de pollo tratados con metilmercurio (2,5 mg), reduciendo en gran medida los efectos teratogénicos observados al inyectar la forma mercurial a los huevos fertilizados (Bekhet et al, 2013).…”