Effects of vitamin E, inorganic selenium, bacterial organic selenium, and their combinations on immunity response in broiler chickens

Abstract

BackgroundSelenium (Se) and vitamin E (Vit E) can act synergistically and affect biological processes, mainly antioxidant and immunity. The use of excess dietary Vit E and Se in animals’ feed could enhance immune response and induce disease resistance. Moreover, different Se sources may provide different alterations in the immune system. Accordingly, the aim of the current study was to assess the impact of dietary supplementation of Vit E, inorganic Se (sodium selenite, SS), bacterial organic Se of ADS18, and their different combinations on the plasma immunoglobulins, ceacum microbial population, and splenic cytokines gene expression in broiler chickens.ResultsPresent results showed that, Se and Vit E synergistic effect was clear in plasma IgM level at day 42 and in splenic cytokines expression (TNF-α, IFN-γ, IL-2, IL-10). The combination of 0.3 mg/kg ADS18-Se with 100 mg/kg Vit E showed the highest IgM level compared to Vit E- SS complex. The combination of either SS or ADS18-Se with Vit E had no significant effect on IFN- γ and IL-10 compared to Vit E alone, while Vit E alone showed the significantly lowest TNF-α compared to the Se combinations. Supplementation of 100 mg/kg Vit E had no effect on microbial population except a slight reduction in Salmonella spp. The main effect of Se sources was that both sources increased the day 42 IgA and IgG level compared to NS group. ADS18-Se modulate the caecum microbial population via enhancing beneficial bacteria and suppressing the E-coli and Salmonella spp. while both Se and Vit E factors had no effect on lymphoid organ weights.ConclusionsThe inclusion of 100 mg/kg Vit E with 0.3 mg/kg ADS18-Se, effectively could support the immune system through regulation of some cytokines expression and immunoglobulin levels more than using ADS18-Se alone, while no difference was observed between using SS alone or combined with Vit E.

Supplemental selenium source on gut health: insights on fecal microbiome and fermentation products of growing puppies

“…In broilers, dietary supplementation with inorganic and bacterial organic selenium reduced the number of E. coli (Dalia et al . 2018 ), when compared to diets without selenium supplementation. Similarly, piglets fed selenium-enriched probiotics had lower E. coli and higher Lactobacillus spp.…”

“… 2008 ), and these bacteria might inhibit pathogenic microorganisms through secretion of hydrogen peroxide, acids and other antimicrobial substances (Dalia et al . 2018 ).…”

“…This observation agreed with a study of Dalia et al . ( 2018 ), which compared the combination of vitamin E with either sodium selenite or bacterial organic selenium for broiler supplementation, observing an increase of DNA concentrations of Lactobacilli, Bifidobacteria and a decrease of E. coli and Salmonella .…”

Examination of the Expression of Immunity Genes and Bacterial Profiles in the Caecum of Growing Chickens Infected with Salmonella Enteritidis and Fed a Phytobiotic

“…Previously, differences in the level of expression of immunity-associated genes were also demonstrated depending on external factors such as heat stress, addition of vitamin and mineral supplements into the diet, etc. [122,123].…”

Effect of Organic and Inorganic Dietary Selenium Supplementation on Gene Expression in Oviduct Tissues and, Antioxidant Capacity of Laying Hens

“…Different strains of microorganisms can be employed to produced organic Se via microbial reduction pathway. Among which is Stenotrophomonas maltophilia (ADS18) isolated from hot-spring water and linked with rich in organic Se-containing proteins which can be applied as Se source in poultry 11,30 . While it is evident that Se can enhance the antioxidant system, scienti c data about the effect of this new organic Se source on layers is limited, and to this edge and to our knowledge, there is no published study reported to investigate the effect of bacterial organic Se of ADS18 source on the expression of some uteri genes and selenoproteins in layers.…”