Effects of washed platelets vs platelet-rich plasma on the proliferation and mineralization of rat dental pulp cells

Zhang, L; Xie, Yuan; Lin, Been‐Ren

doi:10.4238/2015.august.14.12

Cited by 7 publications

(5 citation statements)

References 29 publications

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“…We used low concentrations of CGF extract to mimic the sustained release feature in vivo , and the immunoregulatory effects of 20 and 50% CCM were not significantly different in most experiments. Consistent with our study, previous studies have shown that CGF extract modulated cell differentiation at relatively low concentrations (5–50%), and higher concentrations exerted no or negative effects [ 29 , 31 ].…”

Section: Discussionsupporting

confidence: 93%

Concentrated growth factor regulates the macrophage-mediated immune response

Liu

Zhou

et al. 2021

Regenerative Biomaterials

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Concentrated growth factor (CGF) is a promising regenerative material that serves as a scaffold and adjunct growth factor for tissue engineering. The host immune response, particularly macrophage activity, plays a critical role in injury repair and tissue regeneration. However, the biological effect of CGF on the immune response is not clear. To enrich the theoretical groundwork for clinical application, the present study examined the immunoregulatory role of CGF in macrophage functional activities in vitro. The CGF scaffold appeared as a dense fibrin network with multiple embedded leukocytes and platelets, and it was biocompatible with macrophages. Concentrated bioactive factors in the CGF extract enhanced THP-1 monocyte recruitment and promoted the maturation of suspended monocytes into adherent macrophages. CGF extract also promoted THP-1 macrophage polarization toward the M2 phenotype with upregulated CD163 expression, as detected by cell morphology and surface marker expression. A cytokine antibody array showed that CGF extract exerted a regulatory effect on macrophage functional activities by reducing secretion of the inflammatory factor interleukin (IL)-1β while inducing expression of the chemokine RANTES. Mechanistically, the AKT signaling pathway was activated, and an AKT inhibitor partially suppressed the immunomodulatory effect of CGF. Our findings reveal that CGF induces a favorable immune response mediated by macrophages, which represents a promising strategy for functional tissue regeneration.

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Section: Discussionsupporting

confidence: 93%

Concentrated growth factor regulates the macrophage-mediated immune response

Liu

Zhou

et al. 2021

Regenerative Biomaterials

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“…A few studies pointed out that PRP could improve odontogenic/osteogenic differentiation [13,34,35] and mineralization [11,12] of dental stem cells. PRP promoted mRNA and protein expression of DSPP and DMP-1 in SCAP [13].…”

Section: Discussionmentioning

confidence: 99%

“…PRP is a favorable autologous scaffold that can facilitate cell proliferation and differentiation [10]. Dental pulp stem cells (DPSCs) cultured with a certain concentration of PRP exhibited preferable proliferation, migration, and mineralization [11,12]. SCAP cultured with PRP expressed a high level of odontogenic markers such as dentin matrix protein 1 (DMP-1) and dentin sialophosphoprotein (DSPP) [13].…”

Section: Introductionmentioning

confidence: 99%

Effects of Platelet-Rich Plasma on Proliferation, Viability, and Odontogenic Differentiation of Neural Crest Stem-Like Cells Derived from Human Dental Apical Papilla

Xiang

Guan

et al. 2020

BioMed Research International

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Objective. This study is aimed at evaluating the effects of platelet-rich plasma (PRP) on proliferation, viability, and odontogenic differentiation of neural crest stem-like cells (NCSCs) derived from human dental apical papilla. Materials and Methods. Cells from apical papillae were obtained and then induced to form neural spheres. The expression of NCSC markers p75NTR and HNK-1 in neural sphere cells was detected by immunofluorescence staining. Human PRP was prepared by a 2-step centrifugation method and activated by CaCl2 and thrombin. The concentrations of PDGF-BB and TGF-β1 in whole blood and PRP were measured by an ELISA kit. PRP in five different concentrations (0%, 2.5%, 5%, 10%, and 25%) was applied to culture NCSCs. On the 1st, 3rd, 5th, and 7th days, cell proliferation was evaluated by CCK8. Cell viability was tested by a live/dead staining kit. mRNA and protein expression of DSPP and BMP4 were analyzed by RT-qPCR and western blot, respectively. Statistical analysis was performed by a one-way analysis of variance (ANOVA) test or t-test. Results. Dental apical papilla cells formed neural spheres, from which cells displayed positive expression of p75NTR and HNK-1. The concentrations of PDGF-BB and TGF-β1 in PRP were about 3.5-fold higher than those in whole blood. 5% and 10% PRP significantly promoted proliferation of NCSCs, while 25% and 50% PRP inhibited cell proliferation from Day 3 to Day 7. Low-concentration (2.5%, 5%, and 10%) PRP slightly improved viability of NCSCs on Day 7. On the other hand, high-concentration (25% and 50%) PRP significantly inhibited viability of NCSCs from Day 3 to Day 7. RT-qPCR and western blot results indicated that 10% PRP could promote odontogenic differentiation of NCSCs on Day 7. mRNA and protein expression of DSPP and BMP4 were significantly upregulated in the 10% PRP group compared to those in the control group (P<0.05). Conclusions. PRP is a simply acquirable blood derivative which contains high concentration of growth factors like PDGF-BB and TGF-β1. PRP in a proper concentration could promote proliferation, viability, and odontogenic differentiation of NCSCs derived from human dental apical papilla.

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“…Previously, similar phenomenon was observed in the mineralization process of rat dental pulp cells when induced under different concentrations of PRP. 28 Qin et al 18 suggested that it may be associated with the unsuitable pH of culture medium resulting from containing too much platelets. We thought that the high level of leukocytes, interleukin (IL)-1β, and IL-6 might be responsible for the negative role of high-dosage CGF as well.…”

Section: Discussionmentioning

confidence: 99%

Effects of concentrated growth factor on proliferation, migration, and differentiation of human dental pulp stem cells in vitro

et al. 2018

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Concentrated growth factor, a novel autologous plasma extract, contained various growth factors which promoted tissue regeneration. In this study, we aimed to investigate the biological effects of concentrated growth factor on human dental pulp stem cells. The microstructure and biocompatibility of concentrated growth factor scaffolds were evaluated by scanning electron microscopy. Cell proliferation and migration, odontoblastic and endothelial cell differentiation potential were assessed after exposing dental pulp stem cells to different concentrations (5%, 10%, 20%, 50%, or 80%) of concentrated growth factor extracts. The results revealed that concentrated growth factor scaffolds possessed porous fibrin network with platelets and leukocytes, and showed great biocompatibility with dental pulp stem cells. Higher cell proliferation rates were detected in the concentrated growth factor-treated groups in a dose-dependent manner. Interestingly, in comparison to the controls, the low doses (<50%) of concentrated growth factor increased cell migration, alkaline phosphatase activity, and mineralized tissue deposition, while the cells treated in high doses (50% or 80%) showed no significant difference. After stimulating cell differentiation, the expression levels of dentin matrix protein-1, dentin sialophosphoprotein, vascular endothelial growth factor receptor-2 and cluster of differentiation 31 were significantly upregulated in concentrated growth factor-supplemented groups than those of the controls. Furthermore, the dental pulp stem cell-derived endothelial cells co-induced by 5% concentrated growth factor and vascular endothelial growth factor formed the most amount of mature tube-like structures on Matrigel among all groups, but the high-dosage concentrated growth factor exhibited no or inhibitory effect on cell differentiation. In general, our findings confirmed that concentrated growth factor promoted cell proliferation, migration, and the dental pulp stem cell-mediated dentinogenesis and angiogenesis process, by which it might act as a growth factor-loaded scaffold to facilitate dentin-pulp complex healing.

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Effects of washed platelets vs platelet-rich plasma on the proliferation and mineralization of rat dental pulp cells

Cited by 7 publications

References 29 publications

Concentrated growth factor regulates the macrophage-mediated immune response

Concentrated growth factor regulates the macrophage-mediated immune response

Effects of Platelet-Rich Plasma on Proliferation, Viability, and Odontogenic Differentiation of Neural Crest Stem-Like Cells Derived from Human Dental Apical Papilla

Effects of concentrated growth factor on proliferation, migration, and differentiation of human dental pulp stem cells in vitro

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