Effects of water-borne copper on the survival, antioxidant status, metallothionein-I mRNA expression and physiological responses of the Chinese mitten crab, <em>Eriocheir sinensis</em> (Decapoda: Brachyura) larvae

Abstract

Summary:The lethal concentration of water-borne copper in Chinese mitten crab Eriocheir sinensis larvae was tested by exposing the animals to 0, 0.1, 0.2, 0.3, 0.5 and 0.8 mg Cu L −1 at 20°C for 96 h. The 96-h median lethal concentration (LC 50 ) and its corresponding 95% confident interval was estimated on zoea 1 larvae and megalopa larvae, respectively. Acute dissolved copper toxicity was higher for zoea 1 larvae (0.16 mg L -1 ) than for megalopa larvae (0.21 mg L -1 ). The antioxidant status, metallothionein-I mRNA expression and physiological response of the crab to copper toxicity was further investigated by exposing the megalopa larvae to 0, 0.08 and 0.16 mg Cu L −1 for 96 h. The superoxide dismutase activity, catalase activity, glutathione S-transferase (GST) activity and lipid peroxidation content of megalopa larvae increased concomitantly with the exposure time and copper concentration. MT-I mRNA expression levels were positively correlated with both the concentration and duration of copper exposure. The oxygen consumption and respiratory quotient of megalopa larvae in response to 0.16 mg L -1 copper were significantly higher than those in the control group after 96 h of exposure (P<0.05). The results of this study highlight the potential effects of copper as a common stressor in E. sinensis larvae. MT-I and GST appear to be suitable biomarkers of environmental copper exposure stress in E. sinensis larvae.Keywords: Eriocheir sinensis; copper toxicity; physiological; larvae; antioxidant enzyme; metallothionein.Efectos del cobre disuelto sobre la supervivencia, estado antioxidante, expresión de la metalotionina-I mRNA y la respuesta fisiológica de las larvas del cangrejo de Shangai, Eriocheir sinensis (Decapoda: Brachyura) Resumen: La concentración letal de cobre disuelto se determina mediante la exposición de larvas del cangrejo de Shangai (Eriocheir sinensis) a dosis de 0, 0.1, 0.2, 0.3, 0.5 y 0.8 mg Cu L -1 , a 20°C durante 96 h. La concentración letal media (LC 50 ) y su correspondiente intervalo de confianza del 95% fueron estimados en los estadios larvales zoea I y megalopa. La toxicidad aguda del cobre disuelto fue mayor sobre larvas de zoea I (0.16 mg L -1 ) que sobre las de megalopa (0.21 mg L -1 ). El estado antioxidante, la expresión del mRNA de la metalotionina-I y la respuesta fisiológica del cangrejo a la toxicidad del cobre fueron posteriormente investigados exponiendo las larvas de megalopa a dosis de 0, 0.08 y 0.16 mg Cu L -1 durante 96 h. La actividad de la superóxido dismutasa (SOD), de la catalasa (CAT), de la glutatión-S-transferasa (GST) y la peroxidación lipídica de las larvas de megalopa se incrementó en relación con el tiempo de exposición y la concentración de cobre. Los niveles de expresión de MT-1 mRNA se correlacionaron positivamente con la concentración de cobre y el tiempo de exposición. El consumo de oxígeno y la tasa respiratoria de las larvas de megalopa fueron significativamente más elevadas que las del grupo control (P<0.05) en respuesta a dosis de 0.16 mg Cu L -1 , du...

The effect of copper nanoparticles supplementation on freshwater prawn Macrobrachium rosenbergii post larvae

“…The dose dependent alteration of SOD, CAT, LPO, GOT, and GPT has been reported in M. rosenbergii fed to Cu supplemented diets [13]. Also, Sun et al [41] reported that the insufficient or excess level of dietary Cu produced depressed hepatic SOD, TBARS and GPx activity in the crab, E. sinensis. The sea anemone, Exaiptasia pallid sowed elevated CAT level when exposed to high level of water borne Cu and CuO-NPs [43].…”

“…These results suggest that the Cu-NPs have influenced the production of haemocyte population in experimental prawns. Similarly, dietary Cu supplemented feed fed P. monodon and E. sinensis shown significant elevations in THC and intracellular superoxide anion (O 2 − ) production ratio [7,9,41]. Cu supplemented feed fed H. discus hannai produced better performance in phenol oxidase and lysozyme activity [7].…”

Effects of Copper Exposure on Oxidative Stress, Apoptosis, Endoplasmic Reticulum Stress, Autophagy and Immune Response in Different Tissues of Chinese Mitten Crab (Eriocheir sinensis)

“…High ambient Cu 2+ is a significant threat to the health of E. sinensis. After 24 h of Cu 2+ exposure, the metabolism and osmotic regulation in the gills of E. sinensis are altered [ 21 ], and after 96 h, its molting, growth, and survival are suppressed [ 22 , 23 ]. Although Cu 2+ toxicity in E. sinensis has garnered much attention in recent years, the knowledge of its underlying molecular mechanisms remains limited.…”