Effects of Weathering on Sterol, Fatty Acid Methyl Ester (FAME), and Hydrocarbon Profiles of Biodiesel and Biodiesel/Diesel Blends

Fuller, Stephen; Spikmans, Val; Vaughan, Gary; Guo, Chris

doi:10.1080/15275922.2012.760181

Cited by 7 publications

(4 citation statements)

References 10 publications

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“…However, the Euclidean distance between an extract from soil sample and the corresponding source increased with time. This can be attributed to different weathering resistances of each FAME in diesel 15 , 16 . The weathering effect implies that the diesel source discrimination method using relative FAME composition is subject to a degree of error, especially in the case of highly weathered samples.…”

Section: Resultsmentioning

confidence: 99%

Discrimination methods for diesel origin by analyzing fatty acid methyl ester (FAME) composition in diesel-contaminated soil

Lee

2021

Sci Rep

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The biodiesel containing fatty acid methyl esters (FAMEs) are blended with refined diesel products. Here, we evaluate relative FAME composition ratio as a potential index to discriminate the pollution origin in diesel-contaminated soil. Artificially contaminated soil was prepared to mimic the release of petroleum products using four different refined diesels; in addition, the contaminated soil was put under natural weathering conditions. The variations in the relative FAME composition ratio was compared with those of the corresponding diesel origin using principal component analysis (PCA) for 60 days. All soil samples could be classified into four groups according to diesel origin using two principal components. The proposed method can be used to discriminate the specific diesel pollution origin in contaminated soils.

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Section: Resultsmentioning

confidence: 99%

Discrimination methods for diesel origin by analyzing fatty acid methyl ester (FAME) composition in diesel-contaminated soil

Lee

2021

Sci Rep

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“…The copyright holder for this preprint this version posted February 10, 2022. ; https://doi.org/10.1101/2022.02.09.479836 doi: bioRxiv preprint Biodiesel is composed of fatty acid methyl esters (FAMEs) that are produced through a transesterification reaction with feedstocks of animal, plant, or microbial lipids (8). The FAMEs in biodiesel make the fuel more susceptible to microbial contamination since FAME compounds exist naturally in the environment and are readily degraded by many different microorganisms (9,10). Additionally, hydrocarbons (e.g.…”

Section: Introductionmentioning

confidence: 99%

“…The varying profiles of each biodiesel, therefore, may selection for differing communities of microorganisms that are adapted to preferentially consume specific FAMEs. Microorganisms oxidize FAME using lipases to first hydrolyze the ester bond, converting the FAME to methanol and the corresponding fatty acid, which is then further oxidized to yield ATP (9,16). Different microorganisms have differing capabilities of fatty acid metabolism that depend on fatty acid chain length.…”

Section: Introductionmentioning

confidence: 99%

The Composition of Diesel Fuel Influences the Structure of Microbiological Assemblages in Contaminated Storage Tanks

Floyd

Stamps

Bojanowski

et al. 2022

Preprint

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Microbiological contamination in diesel storage tanks is widespread and under-reported. The contaminating microorganisms can degrade components of the fuel, which contributes to fouling and corrosion. To better understand the connection between the microorganisms that are responsible for this contamination, the composition of affected fuels, and the resulting impact on fuel quality, we conducted a survey of 106 fuel tanks at 17 military bases across the continental United States. Fuel was sampled from each tank and the resident microbial communities were characterized using high throughput sequencing of small subunit ribosomal RNA gene libraries. Fatty acid methyl esters (FAME) and n-alkanes were characterized and quantified using GC-MS to determine their correlation with the presence of microbial taxa. Redundancy Analyses identified which microbial taxa were more prominent in contaminated fuels. Members of the fungal family Trichomaceae were found to be prominent in fuels containing more FAME. Members of the yeast family Debaryomycetaceae were found to be prominent in fuels containing more pentadecanoic and oleic acid methyl esters. These relationships between fungal taxa and fuel components were directly tested in growth experiments with representative isolates of the Trichocomaceae (Paecilomyces AF001) and Debaryomycetaceae (Wickerhamomyces SE3) families. Paecilomyces was capable of growth on linoleic acid methyl ester but unable to grow on pentadecanoic acid methyl ester, while Wickerhamomyces was able to grow on both substrates. Fuel composition may provide some insight into which microorganisms can proliferate but other factors like competition and symbiosis may also drive microbial proliferation, fouling, degradation, and corrosion in diesel fuels.

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“…Comprehensive two‐dimensional gas chromatography (GC×GC) is widely employed in the analysis of complex samples, such as petrol, biofuels, and biomass, owing to its high peak resolution and peak capacity . Generally, the rapid separation in the second column results in highly narrow peak widths ranging between 120 and 600 milliseconds at the baseline.…”

Section: Introductionmentioning

confidence: 99%

Development of two‐dimensional gas chromatography (GC×GC) coupled with Orbitrap‐technology‐based mass spectrometry: Interest in the identification of biofuel composition

et al. 2020

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Comprehensive gas chromatography (GC) has emerged in recent years as the technique of choice for the analysis of volatile and semivolatile compounds in complex matrices. Coupling it with high‐resolution mass spectrometry (MS) makes a powerful tool for identification and quantification of organic compounds. The results obtained in this study showed a significant improvement by using GC×GC‐EI‐MS in comparison with GC‐EI‐MS; the separation of chromatogram peaks was highly improved, which facilitated detection and identification. However, the limitation of Orbitrap mass analyzer compared with time‐of‐flight analyzer is the data acquisition rate; the frequency average was about 25 Hz at a mass resolving power of 15.000, which is barely sufficient for the proper reconstruction of the narrowest chromatographic peaks. On the other hand, the different spectra obtained in this study showed an average mass accuracy of about 1 ppm. Within this average mass accuracy, some reasonable elemental compositions can be proposed and combined with characteristic fragment ions, and the molecules can be identified with precision. At a mass resolving power of 7.500, the scan rate reaches 43 Hz and the GC×GC‐MS peaks can be represented by more than 10 data points, which should be sufficient for quantification. The GC×GC‐MS was also applied to analyze a cellulose bio‐oil sample. Following this, a highly resolved chromatogram was obtained, allowing EI mass spectra containing molecular and fragment ions of many distinct molecules present in the sample to be identified.

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Effects of Weathering on Sterol, Fatty Acid Methyl Ester (FAME), and Hydrocarbon Profiles of Biodiesel and Biodiesel/Diesel Blends

Cited by 7 publications

References 10 publications

Discrimination methods for diesel origin by analyzing fatty acid methyl ester (FAME) composition in diesel-contaminated soil

Discrimination methods for diesel origin by analyzing fatty acid methyl ester (FAME) composition in diesel-contaminated soil

The Composition of Diesel Fuel Influences the Structure of Microbiological Assemblages in Contaminated Storage Tanks

Development of two‐dimensional gas chromatography (GC×GC) coupled with Orbitrap‐technology‐based mass spectrometry: Interest in the identification of biofuel composition

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