Effects of wogonin on the mechanism of melanin synthesis in A375 cells

Chen, Xi; Gu, Ting; Wang, Jinghua; Xiong, Hui; Wang, Ye‐Qiu; Guo-liang, Liu; Qu, Yan; Zhang, Ning

doi:10.3892/etm.2017.5070

Cited by 3 publications

(2 citation statements)

References 12 publications

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“…Hedgehog (Hh) signalling plays an important role in melanoma pathogenesis, its activity being blocked by wogonin, an active component of flavonoids, in HT144 melanoma cells [ 128 ]. It is accepted that wogonin has various inhibitory effects in different melanoma cells, including on invasion and migration of B16F10 cells, melanin synthesis in A375 melanoma cells, or the proliferation and tumour growth of HT144 melanoma cells [ 129 , 130 ]. The anti-inflammatory effect of wogonin in HT144 melanoma is supported by the following activities: (i) pro-inflammatory factors decrease, (ii) anti-inflammatory factors increase, and (iii) inflammatory cytokines expression increase [ 131 ].…”

Section: Inflammatory Microenvironment In Malignant Melanomamentioning

confidence: 99%

The Interplay between Tumour Microenvironment Components in Malignant Melanoma

et al. 2022

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Malignant melanoma has shown an increasing incidence during the last two decades, exhibiting a large spectrum of locations and clinicopathological characteristics. Although current histopathological, biochemical, immunohistochemical, and molecular methods provide a deep insight into its biological behaviour and outcome, melanoma is still an unpredictable disease, with poor outcome. This review of the literature is aimed at updating the knowledge regarding melanoma’s clinicopathological and molecular hallmarks, including its heterogeneity and plasticity, involving cancer stem cells population. A special focus is given on the interplay between different cellular components and their secretion products in melanoma, considering its contribution to tumour progression, invasion, metastasis, recurrences, and resistance to classical therapy. Furthermore, the influences of the specific tumour microenvironment or “inflammasome”, its association with adipose tissue products, including the release of “extracellular vesicles”, and distinct microbiota are currently studied, considering their influences on diagnosis and prognosis. An insight into melanoma’s particular features may reveal new molecular pathways which may be exploited in order to develop innovative therapeutic approaches or tailored therapy.

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Section: Inflammatory Microenvironment In Malignant Melanomamentioning

confidence: 99%

The Interplay between Tumour Microenvironment Components in Malignant Melanoma

et al. 2022

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“…In accordance with previous studies, quercetin, luteolin, kaempferol, wogonin, baicalein, and acacetin inhibit the activation of c-Jun N-terminal kinase and attenuate the activation of the AP-1 transcription factor ( Jang et al, 2008 ; Chen et al, 2008 ; Fong et al, 2010 ; Chen et al, 2012 ; Kim et al, 2012 ; Chen et al, 2013 ; Wang et al, 2014 ). Furthermore, kaempferol blocks the interactions between PD-1 and PD-L1 ( Kim et al, 2020 ) whereas wogonin can down-regulate mRNA expression of ERK2 in melanoma cells ( Chen et al, 2017 ). Thus, QFJDD suppresses PD-L1 expression in LUAD by inhibiting mRNA transcriptions of EGFR and JUN, and increasing the proportions of CD8 + PD-1 + T.…”

Section: Discussionmentioning

confidence: 99%

Qingfei Jiedu decoction inhibits PD-L1 expression in lung adenocarcinoma based on network pharmacology analysis, molecular docking and experimental verification

et al. 2022

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Objective: We aim at investigating the molecular mechanisms through which the Qingfei Jiedu decoction (QFJDD) regulates PD-L1 expression in lung adenocarcinoma (LUAD).Methods: Bioactive compounds and targets of QFJDD were screened from TCMSP, BATMAN-TCM, and literature. Then, GeneCard, OMIM, PharmGKB, Therapeutic Target, and DrugBank databases were used to identify LUAD-related genes. The protein-protein interaction (PPI) network was constructed using overlapping targets of bioactive compounds in LUAD with the Cytoscape software and STRING database. The potential functions and pathways in which the hub genes were enriched by GO, KEGG, and DAVID pathway analyses. Molecular docking of bioactive compounds and key genes was executed via AutoDock Vina. Qualitative and quantitative analyses of QFJDD were performed using UPLC-Q-TOF-MS and UPLC. Expressions of key genes were determined by qRT-PCR, immunoreactivity score (IRS) of PD-L1 was assessed by immunohistochemistry (IHC), while the CD8+PD-1+T% derived from spleen tissues of Lewis lung cancer (LLC) bearing-mice was calculated using flow cytometry (FCM).Results: A total of 53 bioactive compounds and 288 targets of QFJDD as well as 8151 LUAD associated genes were obtained. Further, six bioactive compounds, including quercetin, luteolin, kaempferol, wogonin, baicalein, and acacetin, and 22 hub genes were identified. The GO analysis showed that the hub genes were mainly enriched in DNA or RNA transcription. KEGG and DAVID pathway analyses revealed that 20 hub genes were primarily enriched in virus, cancer, immune, endocrine, and cardiovascular pathways. The EGFR, JUN, RELA, HIF1A, NFKBIA, AKT1, MAPK1, and MAPK14 hub genes were identified as key genes in PD-L1 expression and PD-1 checkpoint pathway. Moreover, ideal affinity and regions were identified between core compounds and key genes. Notably, QFJDD downregulated EGFR, JUN, RELA, HIF1A, NFKBIA, and CD274 expressions (p < 0.05), while it upregulated AKT1 and MAPK1 (p < 0.05) levels in A549 cells. The PD-L1 IRS of LLC tissue in the QFJDD high dose (Hd) group was lower than model group (p < 0.01). CD8+PD-1+T% was higher in the QFJDD Hd group than in normal and model groups (p < 0.05).Conclusion: QFJDD downregulates PD-L1 expression and increases CD8+PD-1+T% via regulating HIF-1, EGFR, JUN and NFκB signaling pathways. Therefore, QFJDD is a potential treatment option for LUAD.

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Anti-melanogenesis effect from Wampee fruit pectin via α-MSH/TRY pathway in A375 cells

Liao

Zhang

et al. 2022

BMC Complement Med Ther

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Background Polysaccharides from wampee have been reported to process various biological activities, while the relationship between structure and bioactivities has been barely addressed. Pectin, an abundant water-soluble polysaccharide in wampee, showed significant antioxidant activity, which was associated with the anti-melanogenic activity. Therefore, this study investigated the physicochemical characteristics and the anti-melanogenesis effect of pectin extracted from wampee fruit in A375 cells. Methods The physicochemical characterization of pectin from wampee fruit was investigated by gel chromatography (GCP), FT-IR spectroscopy, and NMR spectroscopy methods. The anti-melanogenesis effects and mechanism were evaluated by mushroom tyrosine enzyme and human melanin cell model in vitro. Results The results showed that a molecular weight of 5.271 × 105 Da wampee fruit pectin (WFP) were mainly composed of mannose (Man), ribose (Rib), rhamnose (Rha), glucuronic acid (Glc A), glucose (Glc), galacturonic acid (Gal A), galactose (Gal), and arabinose (Ara), which linked with →4)-β-D-Galp-(1 → units. The current study revealed that WFP could significantly suppress mushroom TRY activity in vitro. Furtherly, WFP significantly reduced intracellular and extracellular melanin formation in A375 melanoma cells depending on the presence of alpha-melanocyte stimulating hormone (α-MSH). TRY activity was only inhibited in α-MSH treated A375 cells. Western blot analysis demonstrated that WFP reverse α-MSH induced melanogenesis in A375 melanoma cells, including in down-regulated TRY, TYRP-1, TYRP-2, MITF and CREB expressions. Conclusion These results indicated that WFP could inhibit α-MSH induced melanogenesis in A375 melanoma cells via α-MSH/TRY pathway. In conclusion, these data provided a new perspective to annotate WFP anti-melanogenesis activity mechanism.

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Effects of wogonin on the mechanism of melanin synthesis in A375 cells

Cited by 3 publications

References 12 publications

The Interplay between Tumour Microenvironment Components in Malignant Melanoma

The Interplay between Tumour Microenvironment Components in Malignant Melanoma

Qingfei Jiedu decoction inhibits PD-L1 expression in lung adenocarcinoma based on network pharmacology analysis, molecular docking and experimental verification

Anti-melanogenesis effect from Wampee fruit pectin via α-MSH/TRY pathway in A375 cells

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