Effects of X-ray on fibroblast mechanical properties

Abstract

Concerning the widespread use of X-rays to detect various diseases, such as oral and dental ones, it is essential to study the effects of this radiation on living cells. From the past, genetic effects and cell death because of X-rays have been studied. In addition, the effect of this ionizing radiation on the mechanical properties of the cell and cytoskeleton has been studied, but different results have been obtained based on different models. In this study, post-culture gingival fibroblast cells were classified into two groups of control and radiation with Nano Magnetic Particles functionalized by folic acid. The cells of the radiation group were exposed to X-rays of 3 mGy•cm 2. The specimens were undergone static creep test by a magnetic tweezer. Spring and damper coefficients were obtained based on the viscoelastic solid modeling. The static and dynamic stiffness of the groups was also calculated. The maximum deformation was decreased after radiation from 0.049 ± 0.01 µm to 0.029 ± 0.01 µm and the static stiffness of the model was 1.6 times decreased. Also, the gel point frequency for the control group was 27 Hz and for the radiation group was 7.5 Hz. The results show that the static and dynamic stiffness of the cells decreases after radiation, and less deformation appears in the cells after irradiation. These changes can be due to the breakdown of membrane chemical bonds and activation of actin fibers after radiation.

Experimental and numerical responses of fibroblast and epithelial cells to the frequency of electric toothbrush

“…Additionally, the gel point frequency was estimated to be about 23Hz in the study conducted by Azami et al on dermal broblast cells; this number is higher than that estimated for gingival cells. Nevertheless, the behavior of the cells at higher frequencies was comparable in both studies(Heydarian et al 2019b)(AmirhosseinAzami et al 2019).According to the results of the nite element analysis performed on these cells, the deformation of the cells occurs more often at their gel point frequency than at the operating frequency of the electric toothbrushes.Deformation of additional cells can result in an increase in cell mechanosensory, thereby enhancing tissue strength(Sarasa-Renedo and Chiquet 2005) (Wang et al 2007)(Chiquet et al 2003). In their investigation, Fernando et al demonstrated that stresses could change the function of cellular actin bers, resulting in the maintenance of the cytoskeleton and cellular scaffold regeneration(Valencia et al 2021).…”