Article InformationPeanut is the most important oil seed crop processed from Central Myanmar: Sagaing, Magway and Mandalay Region. Peanut production and utilization have been important due to its high oil content and nutritive value. Peanut oil has a pale yellow and liquid state at room temperature. The oxidative stability and prolonged shelf-life of peanut oil were dependent on the fatty acid compositions of the oil. The aim of this study was to analyze the fatty acid compositions by using the Gas chromatography-mass spectrometry (GC-MS) method for simultaneous determination and quantification of fatty acids from peanut oil by expressing the results in relative and absolute concentrations. Four different types of peanut oil (Pin Pyant 6 Month, Pin Pyant 4 Month, Sinpadethar-11 and Spain-121) were evaluated for the profiling of fatty acid compositions. The analyzed total fatty acid compositions were used to classify the different concentration in four different types of peanut oil in Myanmar. Significant differences were found in fatty acids composition among the different types of peanut oil. Palmitic (16:0), stearic (18:0), oleic (18:1), linoleic (18:2), linolenic (18:3), arachidic (20:0), and behenic (22:0) acids were found obviously in four different types of peanut oil. The total fatty acids were Pin Pyant 6 Month (10787.75 ppm), Pin Pyant 4 Month (10173.83 ppm), and respectively.