Effects of zinc fluoride on inhibiting dentin demineralization and collagen degradation <i>in vitro</i>: A comparison of various topical fluoride agents

Abstract

Root caries is developed because of demineralization followed by enzymatic collagen degradation. This in vitro study aimed to examine the inhibitory efficacy of ZnF2 on dentin demineralization and collagen degradation. Bovine dentin specimens were treated either with ZnF2 or HCl-acidified ZnF2 (ZnF2/HCl) and then demineralized. Anti-demineralization efficacy was assessed by TMR as mineral loss (ΔZ). The efficacy was compared with silver diammine fluoride (SDF), KF, and acidulated phosphate fluoride (APF). For evaluating anti-collagen degradation, EDTA-demineralized dentin specimens were treated by one of four fluoride agents [SDF, APF, ZnF2/HCl, NaF] followed by collagenase challenge. The eroded depth of collagen layer in the lesion was assessed using optical microscope. ΔZ of SDF, KF, ZnF2/HCl, and APF were significantly lower compared with ZnF2 and Control (no treatment). Regarding anti-collagen degradation, SDF and ZnF2/HCl demonstrated a significant difference in the eroded depth compared with Control. Although SDF possessed higher efficacy, ZnF2/HCl might be beneficial as a staining-free agent.

Silver Diamine Fluoride Has Efficacy in Controlling Caries Progression in Primary Teeth: A Systematic Review and Meta-Analysis

“…An increase in the mineral density and in the hardness of the carious dentin was observed in vitro [Mei et al, 2013], which is consistent with dentin remineralization [Mei et al, 2013]. SDF also inhibits the breakdown of the exposed collagen matrix, since it inhibits matrix metalloproteinases and cysteine cathepsins [Mei et al, 2013], probably due to the high concentration of silver [Thanatvarakorn et al, 2016]. This is very important, since the collagen network provides the scaffold for the new remineralization cores.…”

Zinc Inhibits Collagenolysis by Cathepsin K and Matrix Metalloproteinases in Demineralized Dentin Matrix

“…It has been previously shown that high levels of ZnCl 2 (3.33 mg/mL, ∼ 22 m M ) could significantly inhibit the MMP-induced degradation of DOM [Osorio et al, 2011a], and when doped in adhesive resin formulations, no adverse effect on the immediate bond strength to dentin was observed [Osorio et al, 2011b;Toledano et al, 2012aToledano et al, , b, 2016Thanatvarakorn et al, 2016]. The inhibition mechanism has been attributed to changes in the MMP protein configuration due to excess zinc binding, or to the formation of a zinc-hydroxide bridge blocking the catalytic site of the MMPs [Tezvergil-Mutluay et al, 2010].…”

Inhibitory effect of zinc-containing desensitizer on bacterial biofilm formation and root dentin demineralization

“…Accordingly, they increased dentin resistance to bacterial acids, led to reduction in LD and mineral loss. Zinc, fluoride and calcium in the Biounion Nanofiller of CS were able to demonstrate inhibitory effects on dental hard tissue demineralization 10,25,35,36) and enhance remineralization [37][38][39] , in addition to the ability of fluoride to form fluorapatite, which decreases crystal solubility and makes it more resistant to acid attack 40) . Future studies including SEM/EPMA analysis of both materials are essential to understand more about the exact constituents of the formed precipitates.…”

“…After demineralization by artificial biofilm formation, the specimens were cut into approximately 220 μm thick sections using a low-speed diamond saw and then immediately immersed in a 70% aqueous glycerin solution to prevent lesion shrinkage 25) . To perform TMR imaging, the excess glycerin was removed from the sections, then placed on an X-ray glass plate (High Precision Photo Plate, Konica Minolta Photo, Tokyo, Japan), with a 15-step aluminum step wedge.…”