Effects of Zinc Oxide nanoparticles on Streptococcus pyogenes

Liang, Sharolynne Xiao Tong; Wong, Ling Shing; Lim, Yang Mooi; Lee, Poh Foong; Djearamane, Sinouvassane

doi:10.1016/j.sajce.2020.05.009

Cited by 31 publications

(16 citation statements)

References 42 publications

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“…Simlar to the present results, our earlier studies also demonstrated a dose-dependent bacterial growth inhibition by ZnO NPs with a higher growth inhibition of 81.18% on Gram-positive bacterium S. pyogenes compared to 65.73% on Gram-negative bacterium P. aeruginosa, respectively, when treated with 100 μg/ml of ZnO NPs for 24 h ( Liang et al, 2020 ; Dhanasegaran et al, 2022 ).…”

Section: Resultssupporting

confidence: 88%

“…According to Kairyte et al (2013) , treatment of ZnO NPs on L. monocytogenes resulted in bleb formation, which subsequently caused shrinkage of bacterial cells and intracellular component leakage, and eventually the death of bacterial cells. Further, our earlier studies have reported that the disruption in the cell wall integrity by the treatment of ZnO NPs caused cell death in S. Pyogenes and P. aeruginosa, respectively ( Liang et al, 2020 , and Dhanasekran et al, 2022).…”

Section: Resultsmentioning

confidence: 97%

“…The treatment of bacteria with ZnO NPs causes induction of oxidative stress through increased production of reactive oxygen species and the subsequent peroxidation of lipid bilayer in cell membrane, disturbing the integrity of the bacterial cell wall ( Kadiyala et al, 2018 ; Tiwari et al, 2018 :; Djearamane et al, 2020 ). The attachment of ZnO NPs on the bacterial surface is also reported to cause damage to the cell membrane and leakage of cytoplasmic components, resulting in bacterial cell death ( Alekish et al, 2018 ; Liang et al, 2020 ; Dhanasekaran et al, 2022 ).…”

Section: Introductionmentioning

confidence: 99%

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Remedial Aspect of Zinc Oxide Nanoparticles Against Serratia Marcescens and Enterococcus Faecalis

et al. 2022

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Zinc oxide nanoparticles (ZnO NPs) have been widely used in biomedical applications due to their high biocompatibility and low toxicity to humans. The present work aimed to investigate the antibacterial effects of different concentrations of ZnO NPs on two opportunistic pathogens, Serratia marcescens and Enterococcus faecalis. The surface interaction between nanoparticles and bacterial cell wall, and the subsequent morphological alterations on the bacterial surface, were examined through Fourier transform infrared spectroscopy and scanning electron microscope. The energy dispersive X-ray analysis was used to confirm the elemental composition of ZnO NPs and the cellular accumulation of ZnO NPs in bacteria. The growth-inhibitory test demonstrated a dose-dependent growth inhibitory effect of ZnO NPs against both the test bacteria, as the higher concentration of nanoparticles caused the higher bacterial growth inhibition. The results showed that ZnO NPs caused a higher growth inhibition (63.50 ± 2.50%) on the Gram-positive bacterium E. faecalis compared to the Gram-negative bacterium S. marcescens (51.27 ± 4.56%). Fourier transform infrared spectrum revealed the possible involvement of hydroxyl, carboxyl, amides, methylene, and phosphate groups from the biomolecules of bacterial cell wall such as proteins, carbohydrates, lipids, and phospholipids in the interaction of ZnO NPs on bacterial cell surface. Energy dispersive X-ray analysis showed the higher accumulation of ZnO NPs in E. faecalis than S. marcescens analogous to the bacterial growth inhibition. Scanning electron microscopy images confirmed the antibacterial properties of ZnO NPs, showing the loss of integrity of cell membrane and distortion of bacterial cells. Hence, the potential of ZnO NP as an antibacterial agent against S. marcescens and E. faecalis has been confirmed.

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Section: Resultssupporting

confidence: 88%

Section: Resultsmentioning

confidence: 97%

Section: Introductionmentioning

confidence: 99%

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Remedial Aspect of Zinc Oxide Nanoparticles Against Serratia Marcescens and Enterococcus Faecalis

et al. 2022

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“…Current research argues that this depends on the bacterial strain and also on the physico-chemical properties of the ZnO NPs. The proposed mechanisms of ZnO NPs antimicrobial activity are: (i) the change of membrane potential after NPs attachment, resulting in depolarization, and imbalance in the transport system and other membrane function; and (ii) NPs internalization and intracellular release of Zn(II) that can also lead to bacterial cell death, following binding to internal target molecules or reactive oxygen species release [ 10 , 51 ].…”

Section: Discussionmentioning

confidence: 99%

ZnO Nanoparticles-Modified Dressings to Inhibit Wound Pathogens

et al. 2021

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Zinc oxide (ZnO) nanoparticles (NPs) have been investigated for various skin therapies in recent years. These NPs can improve the healing and modulate inflammation in the wounds, but the mechanisms involved in such changes are yet to be known. In this study, we have designed a facile ZnO nano-coated dressing with improved antimicrobial efficiency against typical wound pathogens involved in biofilm and chronic infections. ZnO NPs were obtained by hydrothermal method and characterized by X-ray diffraction, scanning electron microscopy, transmission electron microscopy, and Fourier-transform infrared spectroscopy. Antibacterial and antibiofilm effects were evaluated against laboratory and clinical isolates of significant Gram-negative (Pseudomonas aeruginosa and Escherichia coli) and Gram-positive (Staphylococcus aureus and Enterococcus faecalis) opportunistic pathogens, by quantitative methods. Our results have shown that the developed dressings have a high antibacterial efficiency after 6–24 h of contact when containing 0.6 and 0.9% ZnO NPs and this effect is similar against reference and clinical isolates. Moreover, biofilm development is significantly impaired for up to three days of contact, depending on the NPs load and microbial species. These results show that ZnO-coated dressings prevent biofilm development of main wound pathogens and represent efficient candidates for developing bioactive dressings to fight chronic wounds.

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“…To elucidate the mechanism of this action, Akbar and Anal [81] confirmed the disrupted cell membrane and accumulation of ZnONPs in the cytoplasm by using TEM and SEM images as well as FTIR, XRD analyses. Furthermore, Liang et al [82] reported that FTIR analysis showed the binding of ZnONPs to polypeptides and glycogen from the Streptococcus pyogenes cell wall that lead to its disruption and cell damage.…”

Section: Antimicrobial Activity Mechanism Of Znonpsmentioning

confidence: 99%

Microbial Mediated Synthesis of Zinc Oxide Nanoparticles, Characterization and Multifaceted Applications

Gomaa

2022

J Inorg Organomet Polym

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Nanoparticles have gained considerable importance compared to bulk counterparts due to their unique properties. Due to their high surface to volume ratio and high reactivity, metallic and metal-oxide nanostructures have shown great potential applications. Among them, zinc oxide nanoparticles (ZnONPs) have gained tremendous attention attributed to their unique properties such as low toxicity, biocompatibility, simplicity, easy fabrication, and environmental friendly. Remarkably, ZnONPs exhibit optical, physical, antimicrobial, anticancer, anti-inflammatory and wound healing properties. These nanoparticles have been applied in various fields such as in biomedicine, biosensors, electronics, food, cosmetic industries, textile, agriculture and environment. The synthesis of ZnONPs can be performed by chemical, physical and biological methods. Although the chemical and physical methods suffer from some disadvantages such as the involvement of high temperature and pressure conditions, high cost and not environmentally friendly, the green synthesis of ZnONPs offers a promising substitute to these conventional methods. On that account, the microbial mediated synthesis of ZnONPs is clean, eco-friendly, nontoxic and biocompatible method. This paper reviews the microbial synthesis of ZnONPs, parameters used for the optimization process and their physicochemical properties. The potential applications of ZnONPs in biomedical, agricultural and environmental fields as well as their toxic aspects on human beings and animals have been reviewed.

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Effects of Zinc Oxide nanoparticles on Streptococcus pyogenes

Cited by 31 publications

References 42 publications

Remedial Aspect of Zinc Oxide Nanoparticles Against Serratia Marcescens and Enterococcus Faecalis

Remedial Aspect of Zinc Oxide Nanoparticles Against Serratia Marcescens and Enterococcus Faecalis

ZnO Nanoparticles-Modified Dressings to Inhibit Wound Pathogens

Microbial Mediated Synthesis of Zinc Oxide Nanoparticles, Characterization and Multifaceted Applications

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