Effects of β-Glucans from Aureobasidium pullulans on Cucumber Mosaic Virus Infection in Chili Pepper

Yoon, Ju-Yeon; Gangireddygari, Venkata Subba Reddy; Cho, In-Sook; Chung, Bong Nam; Yoon, Byung-Dae; Choi, Seung‐Kook

doi:10.5423/rpd.2021.27.1.17

Cited by 3 publications

(3 citation statements)

References 35 publications

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“…The extracted samples of one hundred microliters were placed in the capture-antibody coated enzyme-linked immunosorbent assay (ELISA) plate and incubate at room temperature for 4 h. The ELISA plate-coated wells were washed eight times with phosphate buffered saline (pH 7.5 containing 0.05% Tween-20). DAS-ELISA for PMMoV quantification was carried out as described previously ( Yoon et al, 2021 ). DAS-ELISA data were analyzed with analysis of variance.…”

Section: Methodsmentioning

confidence: 99%

Inhibitory Effects of Pepper Mild Mottle Virus Infection by Supernatants of Five Bacterial Cultures in Capsicum annuum L.

et al. 2022

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Pepper mild mottle virus (PMMoV), one of the most prevalent viruses in chili pepper (Capsicum annuum L.) is a non-enveloped, rod-shaped, single-stranded positive-sense RNA virus classified in the genus Tobamovirus. The supernatants of five bacterial cultures (Pseudomonas putida [PP], Bacillus licheniformis [BLI], P. fluorescens [PF], Serratia marcescens [SER], and B. amyloliquifaciens [BA]) were analyzed to find novel antiviral agents to PMMoV in chili pepper. Foliar spraying with supernatants (1:1, v/v) obtained from Luria-Bertani broth cultures of PP, BLI, PF, SER, and BA inhibited PMMoV infection of chili pepper if applied before the PMMoV inoculation. Double-antibody sandwich enzyme-linked immunosorbent assay showed that treatments of five supernatants resulted in 51-66% reductions in PMMoV accumulation in the treated chili pepper. To identify key compounds in supernatants of PP, BLI, PF, SER, and BA, the supernatants were subjected to gas chromatography-mass spectrometry. The 24 different types of compounds were identified from the supernatants of PP, BLI, PF, SER, and BA. The compounds vary from supernatants of one bacterial culture to another which includes simple compounds—alkanes, ketones, alcohols, and an aromatic ring containing compounds. The compounds triggered the inhibitory effect on PMMoV propagation in chili pepper plants. In conclusion, the cultures could be used to further conduct tissue culture and field trial experiments as potential bio-control agents.

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Section: Methodsmentioning

confidence: 99%

Inhibitory Effects of Pepper Mild Mottle Virus Infection by Supernatants of Five Bacterial Cultures in Capsicum annuum L.

et al. 2022

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“…The absorbance value of each sample was measured at 405 nm by an ELISA reader (Titertek, Huntsville, AI, USA) thirty minutes after the addition of 100 μl of the substrate ( p -nitrophenyl phosphate at 1 mg/ml in 10% of diethanolamine pH 9.8). A sample was noted as positive if the OD exceeds 3 times the mean of the negative controls ( Yoon et al, 2021 ).…”

Section: Methodsmentioning

confidence: 99%

Inhibitory Effect of Chitosan and Phosphate Cross-linked Chitosan against Cucumber Mosaic Virus and Pepper Mild Mottle Virus

et al. 2021

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Cucumber mosaic virus (CMV) and Pepper mild mottle virus (PMMoV) causes severe economic loss in crop productivity of both agriculture and horticulture crops in Korea. The previous surveys showed that naturally available biopolymer material – chitosan (CS), which is from shrimp cells, reduced CMV accumulation on pepper. To improve the antiviral activity of CS, it was synthesized to form phosphate cross-linked chitosan (PCS) and compared with the original CS. Initially, the activity of CS and PCS (0.01%, 0.05%, and 0.1% concentration) compound against PMMoV infection and replication was tested using a half-leaf assay on Nicotiana glutinosa leaves. The total number of local lesions represented on a leaf of N. glutinosa were counted and analyzed with phosphate buffer treated leaves as a negative control. The leaves treated with a 0.1% concentration of CS or PCS compounds exhibited an inhibition effect by 40-75% compared with the control leaves. The same treatment significantly reduced about 40% CMV accumulation measured by double antibody sandwich enzyme-linked immunosorbent assay and increased the relative expression levels of the NPR1, PR-1, cysteine protease inhibitor gene, LOX, PAL, SRC2, CRF3 and ERF4 genes analyzed by quantitative reverse transcriptase-polymerase chain reaction, in chili pepper plants.

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“…A sample was noted as positive if the OD exceeds 3 times the mean of the negative controls (Yoon et al, 2021).…”

Section: Double Antibody Sandwich-enzyme Linked Immunosorbent (Das-el...mentioning

confidence: 99%

Inhibitory Activity of <italic>Pseudomonas putida</italic> and <italic>Bacillus licheniformis</italic> Supernatants on PMMoV in Chili Pepper

Gangireddygari

Cho

Choi

et al. 2023

J. People Plants Environ

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Background and objective: Plant viruses are major obstacles to enhancing crop productivity in both agriculture and horticulture throughout the world, resulting in losses of several billion dollars every year. Controlling viruses is arduous, so agrochemicals are widespread. To minimize the usage of those, this study's objective was to assess bacterial cultures supernatants on pepper mild mottle virus (PMMoV) in chili pepper plants and identify its secondary metabolites.Methods: This 48-h grown Pseudomonas putida (PP) and Bacillus licheniformis (BL) cultures supernatants were foliar sprayed separately in chili pepper plants 24-h before PMMoV inoculation (T1), and 24-h before and after PMMoV inoculation (T2), 2wpi (week's post inoculation), the virus titer was determined by using a double antibody sandwich enzyme-linked immunosorbent assay (DAS-ELISA), and the supernatants were extracted with ethyl acetate and concentrated by rota-evaporation before being analyzed in gas chromatography mass spectrometry (GC-MS).Results: The culture supernatants of PP and BL inhibit PMMoV by 43-47% in both the treatments (T1 & T2) compared to the control. The GC-MS chromatogram of two cultures of supernatants identified the molecules of cyclo (Pro-Val), cyclo (Pro-Leu), and cyclo (Phe-Pro). Commercial forms of these three molecules at three concentrations showed a hypersensitive response, ranging from 45-65% for PMMoV in Nicotiana glutinosa.Conclusion: The results revealed that supernatants of PP and BL-containing compounds have biological control of PMMoV in chili pepper plants.

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Effects of β-Glucans from Aureobasidium pullulans on Cucumber Mosaic Virus Infection in Chili Pepper

Cited by 3 publications

References 35 publications

Inhibitory Effects of Pepper Mild Mottle Virus Infection by Supernatants of Five Bacterial Cultures in Capsicum annuum L.

Inhibitory Effects of Pepper Mild Mottle Virus Infection by Supernatants of Five Bacterial Cultures in Capsicum annuum L.

Inhibitory Effect of Chitosan and Phosphate Cross-linked Chitosan against Cucumber Mosaic Virus and Pepper Mild Mottle Virus

Inhibitory Activity of <italic>Pseudomonas putida</italic> and <italic>Bacillus licheniformis</italic> Supernatants on PMMoV in Chili Pepper

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Effects of β-Glucans from Aureobasidium pullulans on Cucumber Mosaic Virus Infection in Chili Pepper

Cited by 3 publications

References 35 publications

Inhibitory Effects of Pepper Mild Mottle Virus Infection by Supernatants of Five Bacterial Cultures in Capsicum annuum L.

Inhibitory Effects of Pepper Mild Mottle Virus Infection by Supernatants of Five Bacterial Cultures in Capsicum annuum L.

Inhibitory Effect of Chitosan and Phosphate Cross-linked Chitosan against Cucumber Mosaic Virus and Pepper Mild Mottle Virus

Inhibitory Activity of &lt;italic&gt;Pseudomonas putida&lt;/italic&gt; and &lt;italic&gt;Bacillus licheniformis&lt;/italic&gt; Supernatants on PMMoV in Chili Pepper

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Inhibitory Activity of <italic>Pseudomonas putida</italic> and <italic>Bacillus licheniformis</italic> Supernatants on PMMoV in Chili Pepper