Effects of β-Lipotropin and β-Lipotropin-derived Peptides on Aldosterone Production in the Rat Adrenal Gland

Matsuoka, Hiroaki; Mulrow, Patrick J.; Franco‐Saenz, Roberto; Li, Choh Hao

doi:10.1172/jci110311

Cited by 62 publications

(22 citation statements)

References 44 publications

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“…In contrast, Kern et al (20) found no effect on plasma aldosterone or plasma cortisol with bolus administrations of up to 15 mg ß-endorphin in four normal human subjects. Our own in vitro results with ß-endorphin speak against a direct effect of this peptide on the adrenal cortex, thereby confirming the findings ofMatsuoka et al (3) in rat adrenal cell preparations.…”

Section: Discussionsupporting

confidence: 91%

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Effects of β-Lipotropin, β-Endorphin, γ2-Melanotropin and Corticotropin on Steroid Production by Isolated Human Adrenocortical Cells

Eggens

Bähr²,

Oelkers³

et al. 1987

Clinical Chemistry and Laboratory Medicine

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Summary:Normal human adrenocortical cells (from multi-organ donors) were incubated with corticotropin (tetracosactide), highly purified human ß-lipotropin, synthetic human ß-lipotropin, y 2 -melanocyte stimulating hormone and ß-endorphin. Corticotropin stimulated cortisol, aldosterone and 18-hydroxycorticosterone production starting at " 12 -10" 11 mol/1 in normal adrenocortical cells. Purified human ß-lipotropin also stimulated steroidogenesis but 100 -lOOOfold higher concentrations of the peptide were needed. In contrast, synthetic human ß-lipotropin was without any effect on steroid production up to concentrations of 10~? mol/1. Synthetie ß-lipotropin (5 10~1 0 mol/1) did not significantly change the dose-response curve for corticotropin (10~1 3 mol/1-10~9 mol/1) versus the three steroids ineasured. y 2 -Melanotropin and ß-endorphin (10~6 mol/1) stimulated the secretion of cortisol, but not of aldosterone.Since synthetic human ß-lipotropin has no effect on human adrenocortical cells, the purified ß-lipotropin must be contaminated with traces of eorticotropin. Since pathologically elevated levels of proopipmelanocortin-derived peptides will rarely exceed plasma concentrations of 10~1 0 mol/1, our findings in vitro do not support a physiological or pathophysiological role of the peptides examined in the regulation of adrenal steroid secretion.

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Section: Discussionsupporting

confidence: 91%

“…seribed a stimulatory effect of synthetic ß-endorphin Stimülatoiy effects of proopiomelanocortin-derived on aldosterone secretion which could not be conpeptides on isolated rat adrenal cells have recently firmed by Matsuoka et al (3). Synthetic Lys-y 3 -mebeen reported:…”

mentioning

confidence: 99%

Effects of β-Lipotropin, β-Endorphin, γ2-Melanotropin and Corticotropin on Steroid Production by Isolated Human Adrenocortical Cells

Eggens

Bähr²,

Oelkers³

et al. 1987

Clinical Chemistry and Laboratory Medicine

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“…These include: f3-endorphin (7), f3-lipotropin (8), a-MSH (9, 10), -y-MSH (I 1, 12), and aldosterone-stimulating factor (13,14). Aldosterone-stimulating factor and y-MSH have both been reported to be elevated in patients with idiopathic aldosteronism (45,46).…”

Section: Discussionmentioning

confidence: 99%

Central serotonergic stimulation of aldosterone secretion.

Shenker¹,

Gross²,

Grekin³

1985

J. Clin. Invest.

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Serotonin stimulates aldosterone secretion both in vitro and in vivo, and serotonin antagonism decreases plasma aldosterone levels in patients with idiopathic aldosteronism. This study was designed to assess the effects of the serotonin precursor, 5-hydroxytryptophan (5HTP), upon aldosterone secretion in man, and to determine whether stimulatory effects of SHTP are mediated through the central nervous system. Oral 5HTP, administered as a single 200-mg dose, increased plasma aldosterone levels from 4.7±0.6 to 13.3±2.8 ng/dl in dexamethasone-pretreated, normal volunteers. Peripheral inhibition of decarboxylation of 5HTP, achieved by pretreatment with carboxydopa, 25 mg three times daily for 3 d, significantly increased the stimulatory effects of 5HTP on aldosterone levels (P < 0.001). No change in aldosterone levels occurred in subjects who received placebo after pretreatment with dexamethasone and carboxydopa. Increased aldosterone was not accompanied by increases in plasma levels of renin activity, potassium, or ACITH. Plasma levels of 5HTP were markedly increased by carboxydopa pretreatment, but peak plasma levels of serotonin were not significantly altered. Four patients with idiopathic aldosteronism all had an increase in plasma aldosterone levels after 5HTP administration, whereas the response in four patients with aldosterone-producing adenoma was variable.Incubation of isolated human and rat adrenal glomerulosa cells with serotonin resulted in increased aldosterone secretion by both sets of cells, whereas 5HTP was ineffective in stimulating aldosterone secretion in vitro. We conclude that central serotonergic pathways are involved in the stimulation of aldosterone induced by administration of 5HTP. This mechanism may be an important etiologic factor in the hypersecretion of aldosterone that occurs in patients with idiopathic aldosteronism.

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“…However, a relatively high peptide dose (10 sg/100 g body wt) was needed to increase aldosterone secretion. Recently, it has been reported that ,LPH and fMSH selectively stimulated aldosterone production in collagenase-dispersed capsular cells from rat adrenal glands (20,21). The C-terminal #lLPH fragments, ,BEND and Met-enkephalin, had no effect on steroidogenesis in this in vitro system.…”

Section: Resultsmentioning

confidence: 71%

“…Furthermore, the amino-terminal region of pro-OLMC has been reported to potentiate slightly but significantly the action of (1-24) ACTH in isolated rat adrenocortical cells (16). In this study synthetic aMSH, synthetic porcine flMSH, synthetic human fEND and highly purified ovine flLPH were found to have no Recently, I3LPH and #lMSH were observed to stimulate aldosterone production in collagenase-dispersed rat capsular adrenal cells (zona glomerulosa cells) without affecting corticosterone production in zona fasciculata and reticularis cells (20,21). Beta-endorphin and Met-or leucine(Leu)-enkephalin had no effect on steroid production by adrenal cells in this system.…”

mentioning

confidence: 64%

Beta endorphin selectively stimulates aldosterone secretion in hypophysectomized, nephrectomized dogs.

Güllner¹,

Gill²

1983

J. Clin. Invest.

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A B S T R A C T We examined the effects of synthetic human /-endorphin (BEND) and a stable methionine (Met)-enkephalin analogue on aldosterone and cortisol secretion rates in anesthetized, hypophysectomized, and nephrectomized dogs and compared them to those of (1-39) ACTH. The circulation of the adrenal glands was completely isolated on the arterial and venous sides (Hilton Pouch). The peptides were infused to deliver 3 pmol/min into the aortic "pouch." Blood was collected from the vena caval pouch, which received blood only from the adrenal gland. Secretion rates of aldosterone and cortisol were calculated as the product of adrenal blood flow and venous steroid concentration. Duplicate steroid measurements were obtained during a control period, at 10, 30, and 50 min of peptide infusion and during a postcontrol period. BEND increased aldosterone secretion rate from 2.4±0.5 ng/ min (mean±SEM) to 3.2±0.9 ng/min at 10 min (N.S.), 8.2±2.5 ng/min (P < 0.05) at 30 min and 11.0±3.7 ng/ min (P < 0.05) at 50 min of infusion. Cortisol secretion rate was not affected by infusion of SEND. Infusion of the stable Met-enkephalin analogue D-alanine2; Metphenylalanine4, Met(O)-enkephalin-ol or saline alone had no effect on aldosterone or cortisol secretion rates. ACTH infusion increased mean aldosterone secretion rate by -215% and significantly stimulated cortisol secretion rate. These results indicate that BEND selectively stimulates aldosterone secretion with a potency similar to that of an equimolar dose of ACTH. INTRODUCTION There is considerable indirect evidence suggesting that an unidentified pituitary factor other than ACTH

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Effects of β-Lipotropin and β-Lipotropin-derived Peptides on Aldosterone Production in the Rat Adrenal Gland

Cited by 62 publications

References 44 publications

Effects of β-Lipotropin, β-Endorphin, γ2-Melanotropin and Corticotropin on Steroid Production by Isolated Human Adrenocortical Cells

Effects of β-Lipotropin, β-Endorphin, γ2-Melanotropin and Corticotropin on Steroid Production by Isolated Human Adrenocortical Cells

Central serotonergic stimulation of aldosterone secretion.

Beta endorphin selectively stimulates aldosterone secretion in hypophysectomized, nephrectomized dogs.

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