Effects ofSolanum malacoxylon on calcium metabolism in patients with chronic renal failure

Mautalén, C.; Ferraro, Clelia; Cabrejas, M.; Landi, Estefanía; Gotlieb, D

doi:10.1007/bf02064154

Cited by 6 publications

(5 citation statements)

References 3 publications

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“…2a-e. In the search for the active agents in plants, leading to severe calcification in cattle, vitamin D metabolites were identified. Soon, their therapeutic utilization was suggested and dried leaves were administered in three clinical trials in patients with renal failure and hypothyroidism [10][11][12]. For the first time, however, purified plant extracts have been characterized in a bioassay for vitamin D-activity and tested in a pre-clinical model for osteoporosis.…”

Section: Discussionmentioning

confidence: 99%

Effect of vitamin D-containing plant extracts on osteoporotic bone

Rosenberg

Wehr

Bachmann³

2007

The Journal of Steroid Biochemistry and Molecular Biology

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Section: Discussionmentioning

confidence: 99%

Effect of vitamin D-containing plant extracts on osteoporotic bone

Rosenberg

Wehr

Bachmann³

2007

The Journal of Steroid Biochemistry and Molecular Biology

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“…Substantial evidence documents that the plant and/or plant extracts are active in monogastrics, and if given in high amounts can serve as a means of increasing plasma 1,25D 3 concentrations [34, 36, 45-50]. There is also an isolated report where the leaf was administered to a handful of human patients with renal failure as a source of 1,25D 3 to prevent secondary renal hyperparathyroidism [51]. This caused a rise in serum Ca 2+ and an increase in intestinal Ca 2+ absorption, indicating the leaf is active in humans as well.…”

Section: Disucussionmentioning

confidence: 99%

Targeted delivery of 1,25-dihydroxyvitamin D3 to colon tissue and identification of a major 1,25-dihydroxyvitamin D3 glycoside from Solanum glaucophyllum plant leaves

Zimmerman

Koszewski

Hoy

et al. 2015

The Journal of Steroid Biochemistry and Molecular Biology

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Leaves of the Solanum glaucophyllum (Sg) plant, indigenous to South America, have long been known for their calcinogenic toxicity in ruminant animals. It was determined the leaves contained glycosidic derivatives of 1,25-dihydroxyvitamin D3 (1,25D3) and liberation of the free hormone by rumen bacterial populations elicited a hypercalcemic response. Our interest in the leaves is predicated on the concept that the glycoside forms of 1,25D3 would target release of the active hormone in the lower gut of non-ruminant mammals. This would provide a means of delivering 1,25D3 directly to the colon, where the hormone has been shown to have beneficial effects in models of inflammatory bowel disease (IBD) and colon cancer. We fed mice for 10 days with variable amounts of Sg leaf. Feeding 7-333 ug leaf/day produced no changes in plasma Ca2+ and 1,25D3 concentrations, and only at > 1000 ug leaf/day did these values become significantly elevated compared to controls. Gene expression studies from colon tissue indicated a linear relationship between the amount of leaf consumed and expression of the Cyp24a1 gene. In contrast, Cyp24a1 gene expression in the duodenums and ileums of these mice was unchanged compared to controls. One of the major 1,25D3-glycosides was isolated from leaves following extraction and purification by Sep-Pak cartridges and HPLC fractionation. Ultraviolet absorbance was consistent with modification of the 1-hydroxyl group, and positive ion ESI mass spectrometry indicated a diglycoside of 1,25D3. 2-Dimensional NMR analyses were carried out and established the C1 proton of the A-ring was interacting with a C1’ sugar proton, while the C3 proton of the A-ring was linked with a second C1’ sugar proton. The structure of the isolated compound is therefore consistent with a beta-linked 1,3-diglycoside of 1,25D3. Thus, Sg leaf administered to mice at up to 333 ug/day can elicit colon-specific enhancement of Cyp24a1 gene expression without inducing hypercalcemia, and the 1,3-diglycoside is one of the major forms of 1,25D3 found in the leaf.

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“…In dairy cows, supplementation with S. glaucophyllum has been proposed to prevent postpartum hypocalcaemia or milk fever (Fricke, 1985; Gast, Horst, Jorgensen, & Luca, 1979). In human medicine, supplementation with calcinogenic plants has been proposed as an alternative to synthetic vitamin D for the treatment of renal failure (Herrath, Kraft, Offermann, & Schaefer, 1974; Mautalen, Ferraro, Cabrejas, Landi, & Gotlieb, 1977).…”

Section: Introductionmentioning

confidence: 99%

The Solanum glaucophyllum Desf. extract reduces mineralized matrix synthesis in osteogenically differentiated rat mesenchymal stem cells in vitro

Melo

Ocarino

Reis

et al. 2020

Animal Physiology Nutrition

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The Solanum glaucophyllum Desf. has been used to treat and prevent diseases in human and veterinary medicine. On the other hand, plant poisoning causes several bone diseases, among them osteoporosis, which is characterized by osteoblastic hypoplasia. Because the osteoblast is a cell derived from the differentiation of mesenchymal stem cells (MSCs) from bone marrow, the hypothesis is that the plant reduces the osteogenic differentiation of MSCs. The objective of this study was to evaluate the effects of S. glaucophyllum Desf. extract on MSCs cultured in osteogenic differentiation medium. We determined by liquid chromatography that 1 ml of plant extract contained 3.8 μl of 1,25(OH)2D3 (calcitriol). Four groups of MSCs cultivated in osteogenic medium were evaluated as follows: (a) treated with 100 μl of extract/L containing 0.4 μg/L of calcitriol; (b) treated with 1 ml of extract/L containing 4 μg/L of calcitriol; (c) treated with 5 ml of extract/L containing 20 μg/L of calcitriol; and (d) a control group without extract. We performed alkaline phosphatase activity assay, analysis of MTT conversion to formazan, and evaluated the percentage of cells, and number and diameter of mineralization nodules. The expression of gene transcripts for osteopontin, bone sialoprotein and BMP‐2 was analysed by RT‐qPCR. After 21 days, there was a significant reduction in MTT conversion to formazan in treated groups, of the cellularity in the group with 5 ml of extract/L, and in the number and size of mineralization nodules in the groups treated with 1 and 5 ml of extract/L. The 5 ml extract/L concentration also reduced transcript expression of osteopontin. It is concluded that S. glaucophyllum Desf. at concentrations of 1 and 5 ml extract/L reduced mineralized matrix synthesis in MSCs cultivated in osteogenic differentiation medium, which suggests that this is one of the mechanisms by which osteoporosis occurs in intoxicated animals.

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Effects ofSolanum malacoxylon on calcium metabolism in patients with chronic renal failure

Cited by 6 publications

References 3 publications

Effect of vitamin D-containing plant extracts on osteoporotic bone

Effect of vitamin D-containing plant extracts on osteoporotic bone

Targeted delivery of 1,25-dihydroxyvitamin D3 to colon tissue and identification of a major 1,25-dihydroxyvitamin D3 glycoside from Solanum glaucophyllum plant leaves

The Solanum glaucophyllum Desf. extract reduces mineralized matrix synthesis in osteogenically differentiated rat mesenchymal stem cells in vitro

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