Effects on gut properties in exocrine pancreatic insufficient (EPI) pigs, being growth retarded due to pancreatic duct ligation at 7 weeks but not at 16 weeks of age

Prykhodko, Olena; Fedkiv, Olexandr; Weström, Björn; Pierzynowski, Stefan

doi:10.1016/j.advms.2013.08.004

Cited by 11 publications

(10 citation statements)

References 34 publications

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“…which has also been confirmed by our lab, using a pig model [3]. In order to understand the purpose of the current study, as well as our hypothesis, one should consider that pancreatic enzymes are not only responsible for digestion, but also exhibit a positive influence on the luminal intestinal mucosa [27,28].…”

Section: Discussionsupporting

confidence: 68%

Enteral Pancreatic-like Enzymes of Microbial Origin affect Insulin Release during an Intravenous Glucose Tolerance Test

Pierzynowski¹,

Goncharova²,

Woliński

et al. 2016

J Diabetes Metab

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We have previously shown that the presence of pancreatic enzymes in the gut lumen of exocrine pancreatic insufficient pigs influences blood glucose and insulin levels during an intravenous glucose tolerance test (IVGTT). The present study aims to highlight the effects of orally applied pancreatic-like enzymes on blood glucose and plasma insulin levels during an IVGTT in young intact pigs.Five, 7-week old pigs were fed with pancreatic-like enzymes of microbial origin, a proteinase (from Aspergillus melleus), α-amylase (from Aspergillus oryzae) or lipase (from Burkholderia cepacia) alone or in combination with the Ca/Na salts of α-ketoglutaric acid (AKG). One hour following administration of the various supplements an IVGTT was performed. Blood samples were withdrawn during the 2 hours of IVGTT for glucose and insulin analyses.Blood glucose during the IVGTT was identical following administration of all combinations of the various enzymes or enzyme mixtures. Enteral loading of amylase or any amylase containing mixture resulted in reduced insulin secretion while administration of proteinase or any proteinase containing mixture resulted in enhanced insulin secretion during IVGTT, as compared to the control water vehicle. Lipase or AKG and lipase or AKG containing mixtures did not affect insulin secretion. Thus, it can be suggested that host amylase/protease ratio and their amount in pancreatic juice can participate in regulation of insulin release, thus, possibly affecting development of obesity and diabetes type 2.

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Section: Discussionsupporting

confidence: 68%

Enteral Pancreatic-like Enzymes of Microbial Origin affect Insulin Release during an Intravenous Glucose Tolerance Test

Pierzynowski¹,

Goncharova²,

Woliński

et al. 2016

J Diabetes Metab

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“…; Prykhodko et al . ). Pancreatic duct ligation results in the dilatation of the pancreatic duct, atrophy of the acinar cells, and their replacement with fibrous tissue in the pancreatic remnant (Imondi et al .…”

Section: Discussionmentioning

confidence: 97%

“…Pancreatic duct ligation in young pigs leads to the immediate elimination of pancreatic enzymes from the gut lumen without affecting bile flow (Prykhodko et al 2014). The main consequence of PDL is the development of EPI.…”

Section: The Porcine Epi Modelmentioning

confidence: 99%

“…The main consequence of PDL is the development of EPI. Exocrine pancreatic insufficiency in young pigs that would normally be growing rapidly is manifested by poor nutrient assimilation and growth retardation Rengman et al 2009;Prykhodko et al 2014). Pancreatic duct ligation results in the dilatation of the pancreatic duct, atrophy of the acinar cells, and their replacement with fibrous tissue in the pancreatic remnant (Imondi et al 1972).…”

Section: The Porcine Epi Modelmentioning

confidence: 99%

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Decreased insulin secretion and glucose clearance in exocrine pancreas‐insufficient pigs

Lozinska

Weström

Prykhodko

et al. 2015

Experimental Physiology

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New Findings r What is the central question of this study? Does the exocrine pancreas have an impact on endocrine pancreatic function and peripheral nutrient utilization? r What is the main finding and its importance?In an exocrine pancreas-insufficient pig model, the insulin response to a glucose load was delayed. Oral enzyme supplementation did not improve the insulin release but facilitated blood glucose clearance. These results suggest an acino-insular axis communication affecting islet function and an impact of gut pancreatic enzymes on blood glucose utilization.The effect of exocrine pancreatic function on the glucose-mediated insulin response and glucose utilization were studied in an exocrine pancreas-insufficient (EPI) pig model. Five 10-week-old EPI pigs after pancreatic duct ligation and 6 age-matched, non-operated control pigs were used in the study. Blood glucose, plasma insulin and C-peptide concentrations were monitored during meal (MGTT), oral (OGTT) and intravenous (IVGTT) glucose tolerance tests. Upon post-mortem examination, the pancreatic remnants of the EPI pigs showed acinar fibrotic atrophy but normal islets and β-cell morphology. The EPI pigs displayed increased fasting glucose concentrations compared with control animals (6.4 ± 0.4 versus 4.8 ± 0.1 mmol l −1 , P < 0.0001) but unchanged insulin concentrations (2.4 ± 0.6 versus 2.1 ± 0.2 pmol l

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“…As exocrine pancreatic function is essential for digestion and absorption of nutrients the complete loss of pancreatic secretion causes maldigestion and malabsorption of various macro- and micronutrients [24,25,26,27]. The juvenile PL pig was used in several studies to test the effects of PEI on growth and different parameters in juveniles [23,28,29,30,31]. Due to the tremendous growth rate of juvenile pigs (birth weight: 1.5 kg; weight at six months: 120 kg) deficiencies develop much faster than in humans.…”

Section: Introductionmentioning

confidence: 99%

Oral Supplementation with a Special Additive of Retinyl Palmitate and Alpha Tocopherol Reduces Growth Retardation in Young Pancreatic Duct Ligated Pigs Used as a Model for Children Suffering from Exocrine Pancreatic Insufficiency

Mößeler

Schmicke

Höltershinken

et al. 2016

IJMS

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Pancreatic exocrine insufficiency (PEI) is a disease of diverse aetiology—e.g., majority of patients suffering from cystic fibrosis (CF) show PEI congenitally. Malnutrition and malabsorption of nutrients impair growth and nutritional status. As reduced fat digestion leads to a deficiency of fat-soluble vitamins the supplementation is standard, but absorption is a critical point in PEI-patients. The pancreatic duct ligated (PL) pig is an established model for PEI in humans and has been proven to be a suitable model to compare different vitamin additives for supplementation. In a former study, PEI caused distinct growth retardation in young piglets, but did not affect growth in older ones. Our study hypothesised that this age-dependent effect is caused by exhausted body reserves of fat-soluble vitamins and, therefore, extra supply reduces growth retardation. PEI was induced by PL at the age of seven (PL-7) or 16 weeks (PL-16). Controls (C) underwent a sham surgery. Some PL-7 pigs (PL-7 + Vit) were fed a special vitamin additive. PEI reduced the mean final body weight (kg) at 26 weeks of age significantly with lower effect in PL-16-pigs (C:117; PL-7:49.5; PL-7 + Vit:77.1; PL-16:96.4). Extra vitamin supply resulted in an increased growth and normalised serum concentration of alpha-tocopherol, underlining the importance of special supplementation in PEI-patients.

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Effects on gut properties in exocrine pancreatic insufficient (EPI) pigs, being growth retarded due to pancreatic duct ligation at 7 weeks but not at 16 weeks of age

Cited by 11 publications

References 34 publications

Enteral Pancreatic-like Enzymes of Microbial Origin affect Insulin Release during an Intravenous Glucose Tolerance Test

Enteral Pancreatic-like Enzymes of Microbial Origin affect Insulin Release during an Intravenous Glucose Tolerance Test

Decreased insulin secretion and glucose clearance in exocrine pancreas‐insufficient pigs

Oral Supplementation with a Special Additive of Retinyl Palmitate and Alpha Tocopherol Reduces Growth Retardation in Young Pancreatic Duct Ligated Pigs Used as a Model for Children Suffering from Exocrine Pancreatic Insufficiency

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