Effects on intestinal microbiota and immune genes of Solea senegalensis after suspension of the administration of Shewanella putrefaciens Pdp11

Vidal, Sara; Tapia‐Paniagua, Silvana; Moriñigo, Jesús Miguel; Lobo, C.; Banda, Inés García de la; Balebona, María del Carmen; Moriñigo, Miguel A.

doi:10.1016/j.fsi.2016.09.010

Cited by 25 publications

(13 citation statements)

References 63 publications

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“…Similarly, the bacterial diversity in the intestine of diseased fish was markedly lower than in healthy fish [ 92 ]. In agreement with our current results, decreased diversity in gut microbiome has also been observed in other fish species with different dietary interventions, such as the combination of probiotic and prebiotic in sole ( Solea senegalensis ) [ 93 ], the replacement of FO by VO in sablefish ( Anoplopoma fimbria ) [ 16 ], or soya inclusion in salmon [ 94 ]. In GSB, the number of OTUs decreased from wild to conventionally reared fish, involving a response of the gut prokaryotic community to the supplied food as well as possible alterations in food assimilation [ 62 ].…”

Section: Discussionsupporting

confidence: 91%

Under control: how a dietary additive can restore the gut microbiome and proteomic profile, and improve disease resilience in a marine teleostean fish fed vegetable diets

et al. 2017

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BackgroundThe constant increase of aquaculture production and wealthy seafood consumption has forced the industry to explore alternative and more sustainable raw aquafeed materials, and plant ingredients have been used to replace marine feedstuffs in many farmed fish. The objective of the present study was to assess whether plant-based diets can induce changes in the intestinal mucus proteome, gut autochthonous microbiota and disease susceptibility of fish, and whether these changes could be reversed by the addition of sodium butyrate to the diets. Three different trials were performed using the teleostean gilthead sea bream (Sparus aurata) as model. In a first preliminary short-term trial, fish were fed with the additive (0.8%) supplementing a basal diet with low vegetable inclusion (D1) and then challenged with a bacteria to detect possible effects on survival. In a second trial, fish were fed with diets with greater vegetable inclusion levels (D2, D3) and the long-term effect of sodium butyrate at a lower dose (0.4%) added to D3 (D4 diet) was tested on the intestinal proteome and microbiome. In a third trial, the long-term effectiveness of sodium butyrate (D4) to prevent disease outcome after an intestinal parasite (Enteromyxum leei) challenge was tested.ResultsThe results showed that opposed forces were driven by dietary plant ingredients and sodium butyrate supplementation in fish diet. On the one hand, vegetable diets induced high parasite infection levels that provoked drops in growth performance, decreased intestinal microbiota diversity, induced the dominance of the Photobacterium genus, as well as altered the gut mucosal proteome suggesting detrimental effects on intestinal function. On the other hand, butyrate addition slightly decreased cumulative mortality after bacterial challenge, avoided growth retardation in parasitized fish, increased intestinal microbiota diversity with a higher representation of butyrate-producing bacteria and reversed most vegetable diet-induced changes in the gut proteome.ConclusionsThis integrative work gives insights on the pleiotropic effects of a dietary additive on the restoration of intestinal homeostasis and disease resilience, using a multifaceted approach.Electronic supplementary materialThe online version of this article (10.1186/s40168-017-0390-3) contains supplementary material, which is available to authorized users.

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Section: Discussionsupporting

confidence: 91%

Under control: how a dietary additive can restore the gut microbiome and proteomic profile, and improve disease resilience in a marine teleostean fish fed vegetable diets

et al. 2017

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“…4). The stable presence of prokaryotes in the intestinal tract is not only due to transitory diet since Shewanella strains have been shown to act as probiotics to protect eukaryotes from pathogens (Vidal et al 2016). Metazoans could also be supplied with eicosapentaenoic acid (EPA) and omega-3 fatty acids produced by bacteria (Hau and Gralnick 2007;Dailey et al 2016).…”

Section: Metazoanmentioning

confidence: 99%

TheShewanellagenus: ubiquitous organisms sustaining and preserving aquatic ecosystems

Lemaire

Méjean

Iobbi‐Nivol

2020

FEMS Microbiology Reviews

109

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The Gram-negative Shewanella bacterial genus currently includes about 70 species of mostly aquatic γ-proteobacteria, which were isolated around the globe in a multitude of environments such as surface freshwater and the deepest marine trenches. Their survival in such a wide range of ecological niches is due to their impressive physiological and respiratory versatility. Some strains are among the organisms with the highest number of respiratory systems, depending on a complex and rich metabolic network. Implicated in the recycling of organic and inorganic matter, they are important components of organism-rich oxic/anoxic interfaces, but they also belong to the microflora of a broad group of eukaryotes from metazoans to green algae. Examples of long-term biological interactions like mutualism or pathogeny have been described, although molecular determinants of such symbioses are still poorly understood. Some of these bacteria are key organisms for various biotechnological applications, especially the bioremediation of hydrocarbons and metallic pollutants. The natural ability of these prokaryotes to thrive and detoxify deleterious compounds explains their use in wastewater treatment, their use in energy generation by microbial fuel cells and their importance for resilience of aquatic ecosystems.

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“…In both trials, the authors used denaturing gradient gel electrophoresis (DGGE; a DNA fingerprinting technique) to analyse the composition of microbial communities and detected shifts in the gut microbial community of the treated fish compared with the control fish. This shift was associated with upregulation of immune‐related genes (Cordero et al 2015; Vidal et al 2016). Cordero et al (2015) also investigated haemolytic activity (0%), respiratory burst (−20%) and phagocytic activity (+6.2%) in fish but none of these were significantly different from the control fish.…”

Section: Additional Insights Into the Effect Of Seaweeds On Immune Rementioning

confidence: 99%

“…Gut microbiome and innate immunity in farmed finfish The gut microbiota of fish has a strong influence on their metabolism, nutrition and immunity (Egerton et al 2018). However, in the 142 publications reviewed, only four investigated the effects of the treatments on gut microbiome as well as the innate immune functions (Cordero et al 2015;Vidal et al 2016;Sutili et al 2019;Asaduzzaman et al 2019). The two earlier studies used the probiotic Shewanella putrefaciens Pdp1 supplemented with sodium alginate (brown seaweed extract) in their treatments.…”

Section: Additional Insights Into the Effect Of Seaweeds On Immune Rementioning

confidence: 99%

Meta‐analysis of the use of seaweeds and their extracts as immunostimulants for fish: a systematic review

Thépot

Campbell

Rimmer

et al. 2020

Reviews in Aquaculture

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Disease is an enduring threat to aquaculture, with direct costs from outbreaks of more than US$6 billion a year. Efforts to reduce antibiotic use in aquaculture have resulted in a recent shift towards the use of immunostimulants that boost fish immune systems. Seaweeds are a potential source of unique immunostimulants because of their diverse taxonomy (11,353 species) and diverse bioactives, but there has been no synthesis of their effects on fish. A systematic review and meta‐analysis of 142 peer‐reviewed studies was conducted to evaluate the effects of seaweed on the innate immunity, resistance (to stress and pathogens) and growth of farmed fish. Overall, our review indicated that dietary supplementation with seaweeds or their extracts had substantial positive impacts on the health and growth of fish compared with control groups and were comparable to currently utilised immunostimulants. Dramatic effects on survival of fish during challenge experiments were detected, with an average of 33% higher survival of fish fed diets that included seaweeds or their extracts. This study provides broad evidence for the benefits of supplementing fish feed with the reviewed seaweeds or their bioactive extracts, including potential synergistic effects of supplements. However, the meta‐analysis also highlighted considerable variation among studies, and between fish and seaweed species, making it difficult to extrapolate results beyond individual experiments. Future studies should focus on developing synergistic combinations of ingredients and elucidating the mode of action through isolation of seaweed bioactives, together with standardisation of experimental conditions, to fast‐track the benefits of seaweed supplements for aquaculture.

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Effects on intestinal microbiota and immune genes of Solea senegalensis after suspension of the administration of Shewanella putrefaciens Pdp11

Cited by 25 publications

References 63 publications

Under control: how a dietary additive can restore the gut microbiome and proteomic profile, and improve disease resilience in a marine teleostean fish fed vegetable diets

Under control: how a dietary additive can restore the gut microbiome and proteomic profile, and improve disease resilience in a marine teleostean fish fed vegetable diets

TheShewanellagenus: ubiquitous organisms sustaining and preserving aquatic ecosystems

Meta‐analysis of the use of seaweeds and their extracts as immunostimulants for fish: a systematic review

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