This work focused on the evaluation of antifungal activity of Thymus vulgaris, Cymbopogon citratus and Cupressus sempervirens essential oils formulations based on cassava starch and corncob biochar against fungi associated with cowpea seeds (Vigna unguiculata L., Walp) in storage. 200 cowpea seeds were distributed among plastic bottles, 5 g of the starch-based essential oil formulation, for each dose, were incorporated into the seeds. For the formulation of biochar-based essential oils, each piece of biochar soaked with an essential oil dose was inserted into the bottles so as to be in the middle of the seeds.Treated and untreated seeds were kept at laboratory ambient temperature of 25 o C ± 2 for 70 days. At intervals of 10, 40, 60 and 70 days of storage, fungal infection rates were assessed by using Agar plate method and Blotter technique. The results showed that 8 fungal species were isolated from cowpea seeds including Aspergillus flavus, A. niger, A. parasiticus, Cercospora sp, Cladosporium sp, Fusarium sp, and Rhizopus stolonifer and the percentages of seed infection caused by these different species range from 4 to 51%. Anfifungal effect of starch-based formulations showed that Thymus vulgaris essential oil has the highest antifungal activity with 1.66 % and 2% of cowpea seeds infection respectively after 60 and 70 days of storage, followed by Cupressus sempervirens and Cymbopogon citratus at 7µl/g concentration. For the biochar-based formulation, the essential oil of C. citratus at the dose of 7µl/g was the one that showed the highest antifungal activity with 9% of seeds infected after 70 days of storage. Based on these results, starch and biochar can be used for the formulation of essential oils for the preservation of cowpea seeds. Thymus vulgaris essential oil is the most effective especially when formulated with starch.