Efficacy and Safety of Ashwagandha Root Extract on Cognitive Functions in Healthy, Stressed Adults: A Randomized, Double-Blind, Placebo-Controlled Study

Kumarpillai, Gopukumar; Thanawala, Shefali; Venkateswarlu, Somepalli; Rao, Tejas; Thamatam, Vijaya Bhaskar; Chauhan, Sanjaya

doi:10.1155/2021/8254344

Cited by 45 publications

(43 citation statements)

References 33 publications

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“…Although the current study was conducted in tissue culture in rat embryonic hippocampal neurons, the results reported herein nevertheless provide a plausible molecular mechanism underlying recent clinical findings that WS improves psychological and cognitive effects in depressed patients [26,37,38] possibly through the hypothalamus-adrenal-pituitary axis [29]. Moreover, Manchanda and Kaur [39] have reported that hippocampal BDNF mRNA and phospho-Akt was increased in rats fed WS leaf extracts, relative to those fed a low-fat diet, albeit not statistically so.…”

Section: Discussionmentioning

confidence: 89%

Ashwagandha and Its Active Ingredient, Withanolide A, Increase Phosphorylation of TrkB in Cultured Hippocampal Neurons

Chen

Russo-Neustadt²

2023

EJMP

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Aims: To primary rat embryonic hippocampal neurons in culture, ashwagandha or one of its active ingredients, withanolide A were added in the presence or absence of nutrient supplementation and then assayed for activity of the BDNF receptor, TrkB. Study Design: Primary hippocampal neurons were cultured and grown in nutrient-rich or nutrient-poor medium. Ashwagandha or withanolide A were then be added to both types of media with or without an inhibitor of TrkB or either the PI-3K or MAPK pathway. Place and Duration of Study: Department of Biological Sciences, California State University, Los Angeles, CA, USA, between July 2021 and August 2022. Methodology: Rat embryos were removed by cesarean section from mother rats at 18 days’ gestation and the hippocampi of the former dissected, plated into culture dishes, and treated with the appropriate drug(s) (see Study Design above). After 4 days, neurons were harvested for Western blotting. Optical density of Western blot bands were quantified and statistically analyzed in a 2-way ANOVA, using a level of statistical significance at P < .05. Results: Under normal conditions (with N2 supplement), ashwagandha, but not withanolide A, increased phospho-TrkB immunoreactivity when compared to the effects of vehicle (controls, F(11, 24) = 22.48, P < .001), although withanolide A did not quite reach statistical significance (P = .069) when compared to that of the controlled condition. Likewise, under nutrient-deprived conditions, both ashwagandha and withanolide A also increased phosphorylation of TrkB when compared to that of vehicle-nutrient-deprived conditions (P < .0001). The same results were obtained in the presence of inhibitors of TrkB itself and the PI-3K (ashwagandha, P < .001; withanolide A, P < .001) and MAPK (ashwagandha, P = .027; withanolide A, P = .045) pathways. Conclusion: Ashwagandha or withanolide A activates TrkB, in nutrient-deprived hippocampal neurons, underscoring its role in neuronal survival signaling.

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Section: Discussionmentioning

confidence: 89%

Ashwagandha and Its Active Ingredient, Withanolide A, Increase Phosphorylation of TrkB in Cultured Hippocampal Neurons

Chen

Russo-Neustadt²

2023

EJMP

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“…Subiektywną poprawę jakości snu wśród osób zdrowych odnotowano w badaniu nad wpływem W. somnifera na funkcje kognitywne u zdrowych, zestresowanych ludzi [17]. Proporcjonalność poprawy subiektywnej oceny jakości snu wraz z zwiększającą się dawką preparatu została opisana w jednym randomizowanym, przeprowadzonym metodą podwójnie ślepej próby badaniu porównawczym przeprowadzonym przez Salve J et al (2019) [18].…”

Section: Badania Na Ludziachunclassified

Ashwagandha (Withania somnifera) - influence on sleep: review

Jędruszczak

Sylwia

Walczak

et al. 2023

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Introduction: Ashwagandha (Withania somnifera) is a plant found on the Indian subcontinent. The health-promoting properties attributed to it have been largely verified in numerous studies over the past 20 years. This article reviews studies on the effects of ashwagandha supplementation on sleep, its deprivation, and studies analyzing the mechanisms determining such effects. Materials and methods: A review of the literature available in the PubMed database was carried out, using the key words: "ashwagandha" ; "sleep" ; "insomnia", "withania", "somnifera", "withanolide", "withaferin". Results: Ashwagandha has a positive effect on the quality and length of sleep. It is indicated that it improves sleep parameters among both insomniacs and healthy people. In addition, it has been proven to reduce oxidative stress under conditions of sleep deprivation. Ashwagandha's positive effects on sleep have been shown to be related to its effects on GABA-nergic transmission, and the chemical responsible for the plant's sleep induction is triethylene glycol. Its regular use increases dopamine levels in the brain and could potentially be useful in treating the sleep-related disorder restless legs syndrome. Conclusions: The use of ashwagandha is a potential direction for treating sleep disorders and improving sleep quality in healthy individuals. There is a need for further development and studies on larger groups of patients to exploit its potential.

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“…The extract of this drugs induce the dendrites and axon outgrowth and its show the neuronal regeneration. 10…”

Section: Chemical Compositionsmentioning

confidence: 99%

Effect of Withania Somnifera (L.) root extract\'s on PC12 induced by hydrogen peroxide, study

Kumar¹,

Dhariwal²,

Kumar³

et al. 2023

JPBS

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The L. is also used as a crude medicament that mostly effected in various neurological and immunological disorders. It is also known as Ashwagandha and “Indian ginseng” in Indian region. There is a lack of information on the possible neuroprotective properties of W. somnifera root against HO- and Ab(1-42)-induced cytotoxicity, which are now targeted for innovative treatments for dementia, particularly dementia of the Alzheimer's type (AD). According to this research, we prepared an aqueous extract of dried roots of W. somnifera that possess the protective effect against Ab- aggregated fibril and HO cytotoxicity through MTT assay with the help of differentiated rat pheochromocytoma PC12 cell. The findings indicate that, in a concentration-dependent manner, pretreatment of differentiated PC12 cells with aqueous extracts of W. somnifera root strongly protects differentiated PC12 cells against both H2O2- and Ab(1-42)-induced cytotoxicity. The W. somnifera extract was examined using liquid chromatography-serial mass spectrometry in order to look into the substances that could be responsible for the effects that were seen. Withanolide derivatives, notably withaferin A, were found in abundance. These findings support the hypothesis that W. somnifera may be used ethnopharmacologically to treat oxidative stress-related cognitive and other neurodegenerative illnesses. They also show the neuroprotective activities of an aqueous extract of W. somnifera root.

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Efficacy and Safety of Ashwagandha Root Extract on Cognitive Functions in Healthy, Stressed Adults: A Randomized, Double-Blind, Placebo-Controlled Study

Cited by 45 publications

References 33 publications

Ashwagandha and Its Active Ingredient, Withanolide A, Increase Phosphorylation of TrkB in Cultured Hippocampal Neurons

Ashwagandha and Its Active Ingredient, Withanolide A, Increase Phosphorylation of TrkB in Cultured Hippocampal Neurons

Ashwagandha (Withania somnifera) - influence on sleep: review

Effect of Withania Somnifera (L.) root extract\'s on PC12 induced by hydrogen peroxide, study

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