Efficacy and safety profile of a subtilisin protease produced by fermentation in Bacillus licheniformis to be used as a feed additive

Cupi, Denisa; Thorsen, Michael; Elvig-Jørgensen, Signe Gry; Wulf-Andersen, Linda; Berti-Sorbara, Jose-Otavio; Cowieson, A. J.; Faruk, Murtala Umar

doi:10.1016/j.heliyon.2022.e10030

Cited by 7 publications

(7 citation statements)

References 31 publications

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“…The protease used in this study was a novel subtilisin protease produced by Bacillus licheniformis ( Cupi et al, 2022 ). This is a sfericase protease, an endopeptidase from the serine protease family, subtilisin subfamily A, MEROPS ID S08.113 ( Rawlings et al., 2014 ).…”

Section: Materials and Mehodsmentioning

confidence: 99%

“…This is a sfericase protease, an endopeptidase from the serine protease family, subtilisin subfamily A, MEROPS ID S08.113 ( Rawlings et al., 2014 ). Activity for this protease is defined in New Feed Protease ( NFP ) units, which measures the enzyme amount required to hydrolyze 1 µmol of para-nitroaniline ( pNA ) from 1 M 128 substrate Suc-Ala-Ala-Pro-Phe-pNA ( Cupi et al, 2022 ). Enzyme activity in the present study was conducted in an Infinite M200 Pro microtiter plate reader (Tecan Lifesciences, Männedorf, Switzerland) with the amount of released yellow pNA being proportional to the protease activity of the enzyme measured photometrically at a wavelength of 405 nm.…”

Section: Materials and Mehodsmentioning

confidence: 99%

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Effects of exogenous protease on performance, economic evaluation, nutrient digestibility, fecal score, intestinal morphology, blood profile, carcass trait, and meat quality in broilers fed normal diets and diets considered with matrix value

Lee¹,

Oh²,

Kim³

et al. 2023

Poultry Science

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Section: Materials and Mehodsmentioning

confidence: 99%

Section: Materials and Mehodsmentioning

confidence: 99%

Effects of exogenous protease on performance, economic evaluation, nutrient digestibility, fecal score, intestinal morphology, blood profile, carcass trait, and meat quality in broilers fed normal diets and diets considered with matrix value

Lee¹,

Oh²,

Kim³

et al. 2023

Poultry Science

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“…The tested protease was a novel subtilisin protease from Bacillus sp. produced in Bacillus licheniformis (Cupi et al, 2022). This is a sfericase protease, an endopeptidase from the serine protease family, subtilisin subfamily A, MEROPS ID S08.113 (Rawlings et al, 2014).…”

Section: Supplemental Proteasementioning

confidence: 99%

“…This is a sfericase protease, an endopeptidase from the serine protease family, subtilisin subfamily A, MEROPS ID S08.113 (Rawlings et al, 2014). Activity for this protease is defined in New Feed Protease units (NFP), which measures the enzyme amount required to hydrolyze 1 µmol of para-nitroaniline (pNA) from 1 M substrate Suc-Ala-Ala-Pro-Phe-pNA (Cupi et al, 2022). Enzyme activity in the feeds of the present study were conducted in an Infinite M200 Pro microtiter plate reader (Tecan Lifesciences, Switzerland), with the amount of released yellow pNA being proportional to the protease activity of the enzyme measured photometrically at a wavelength of 405 nm.…”

Section: Supplemental Proteasementioning

confidence: 99%

“…Exogenous proteases that can compete and succeed commercially are required to function at corresponding pH's in the gastrointestinal tract, but they also must sustain digestive capabilities after being heat treated at temperatures regularly used in feed processing mills (Ravindran, 2013). Broad substrate specificity of some exogenous proteases, as well as their higher concentrations at the entry of digestive tracts, may produce benefits as effective catalyzers, enabling them to break peptides down faster when compared to feeds not supplemented with proteases (Ravindran, 2013;Cupi et al, 2022).…”

Section: Introductionmentioning

confidence: 99%

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Growth performance and nutrient digestibility of broiler chickens as affected by a novel protease

et al. 2023

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Two experiments evaluated the addition of an exogenous sfericase protease in broiler diets. Experiments were run (Exp1 and Exp2) with 1,848 and 2,100 one-day-old male chicks being allocated into 84 floor pens with 14 replicates of 22 and 25 birds each, respectively. The studies were conducted in completely randomized designs. In Exp1, Standard diets were formulated with energy and AA at marginally lower levels than usual by the Brazilian integration such that broilers were expected to grow at comparatively reduced rates to the industry whereas in Exp2, the Standard diets were formulated using energy and AA as usual by the Brazilian integrations such that broilers were expected to grow comparable to industry rates. Standard diets had ideally balanced amino acids (AA). Matrix diets, in contrast, had reductions of 6% digestible lysine and of 20 kcal AME/kg compared to the Standard. Matrix diets were supplemented with an sfericase protease at 0, 10,000, and 30,000 New Feed Protease units (NFP)/kg. Outcomes showed no interaction between diet and protease in any of the experiments. However, broilers fed Standard diets had higher cumulative body weight gain (BWG) to 35 and 42 d when compared to Matrix fed birds whereas FCR were worse for birds fed the Matrix diets at 35 d in EXP1 and at 35 and 42 d in EXP2. Improvements in FCR were observed when the sfericase protease was added throughout all ages in EXP1 with a beneficial trend (P<0.067) observed in the cumulative FCR at 42 d in EXP2. The ileal digestible crude protein (IDCP) was significantly higher for birds fed Standard feeds in EXP1 with no other differences in digestibility found in any of the experiments. Protease addition led to improvements in ileal digestibility of dry matter (IDM) and IDCP (P < 0.05) compared to no protease addition in EXP1 as well as in ileal digestibility of energy (IDE) when 30,000 protease units were added. The present report demonstrates that the novel sfericase protease was successful in compensate broiler performance when reductions of 6% digestible Lys and 20 kcal/kg AME were imposed. This compensation, however, seemed more notable when birds were fed diets formulated to support moderate rather than maximum growth and having animal protein in the feed formula.

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Research progress of probiotics and fermented feed effects on pork quality

Lian,

Shi,

Lin

et al. 2024

Food Bioengineering

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With the growth of the global population and the development of the economy, the demand for delicious and nutritious meat is increasing. However, the pursuit of high productivity and a high lean meat rate in pig breeding has led to a serious decline in meat quality. How to moderately improve the quality and flavor of pork through nutritional supplementation is of great significance in meeting consumers' demand for high‐quality and delicious pork. Recent studies have shown that fermented feed using probiotics can not only improve the nutritional quality and utilization rate of agricultural by‐products, but also play an important role in improving meat color, increasing intramuscular fat content, regulating the flavor of pork, leading to a sustainable development of agriculture and husbandry. In this paper, we will review the current situation of the probiotics used in fermented feed, the effects of fermentation on the improvement of feed quality, the influence and mechanism of fermented feed on the growth performance, meat quality, and flavor of pigs, and so forth, aiming to provide theoretical basis and technical support for the research and development of fermented feed and its application in the production of high‐quality pork.

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Efficacy and safety profile of a subtilisin protease produced by fermentation in Bacillus licheniformis to be used as a feed additive

Cited by 7 publications

References 31 publications

Effects of exogenous protease on performance, economic evaluation, nutrient digestibility, fecal score, intestinal morphology, blood profile, carcass trait, and meat quality in broilers fed normal diets and diets considered with matrix value

Effects of exogenous protease on performance, economic evaluation, nutrient digestibility, fecal score, intestinal morphology, blood profile, carcass trait, and meat quality in broilers fed normal diets and diets considered with matrix value

Growth performance and nutrient digestibility of broiler chickens as affected by a novel protease

Research progress of probiotics and fermented feed effects on pork quality

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