Efficacy, Biocompatibility and Degradability of Carbon Nanoparticles for Photothermal Therapy of Lung Cancer

Abstract

Aim: To investigate near infrared-induced phototoxicity toward lung cancer cells, and the biodegradability and effect on immune cells of glucose-derived carbon nanoparticles (CNPs). Methods: The human A549 lung adenocarcinoma cell line was used as a model to study the phototoxicity of CNPs. The biodegradability and the effect on immune cells was demonstrated in primary human neutrophils and macrophages. Results: Near infrared-activated CNPs elicited rapid cell death, characterized by the elevation of heat shock proteins and the induction of DNA damage. CNPs were found to be noncytotoxic toward primary human macrophages and were susceptible to biodegradation when cocultured with human neutrophils. Conclusions: Our results identify CNPs as promising platforms for photothermal therapy of lung cancer.

Molecular Aspects of the Interaction with Gram-Negative and Gram-Positive Bacteria of Hydrothermal Carbon Nanoparticles Associated with Bac8c2,5Leu Antimicrobial Peptide

“…In fact, they exhibit no cytotoxicity against the cells of the immune system and are hemocompatible . Finally, previous data suggest that CNPs are not biopersistent due to degradation by neutrophils …”

“… 15 Finally, previous data suggest that CNPs are not biopersistent due to degradation by neutrophils. 16 …”

“…15 Finally, previous data suggest that CNPs are not biopersistent due to degradation by neutrophils. 16 The aim of this study was the preparation of a nanoformulation based on the physical conjugation of CNPs with the antimicrobial peptides Bac8c 2,5 Leu , and the evaluation of the antibacterial activity of CNPs and peptide-loaded CNPs against Staphylococcus aureus (S. aureus) and Escherichia coli (E. coli). Both strains are already well characterized in literature and usually used as models for Gram-positive (Gram+) and Gram-negative (Gram−) organisms, respectively.…”

Photoresponsive Inorganic Nanomaterials in Oncology

“…114 In addition, previous data suggest that HCNPs are slowly degraded by neutrophils. 56 Finally, depending upon the bulk structure, some of these materials exhibit physical properties like fluorescence, PT and PD capabilities suggest a possible use as both therapeutic and diagnostic agents themselves. 111 Previous studies by some of us found that, when irradiated with an NIR laser beam (945 nm, 1.3 W/cm 2 ), HCNPs efficiently generate heat 112 (Figure 5).…”

Biological interactions of ferromagnetic iron oxide–carbon nanohybrids with alveolar epithelial cells

“…58 On the other hand, the carbon shell prevent the rapid release of iron ions in the intracellular compartments that may induce oxidative stress. 62 Carbon nanomaterials are known to be degraded by myeloperoxidase, 65,66 the enzyme expressed mainly in neutrophils, which generates the strong oxidant hypochlorous acid, and by hydrogen peroxide in macrophages. 67,68 To investigate the possible degradation of the carbon shell by these oxidants, the sample has been incubated in NaClO or H 2 O 2 at two different concentrations (0.2 and 2 mM).…”