Efficacy of a Commercial Enzyme Immunoassay Kit for the Detection of Staphylococcal Enterotoxins in Foods

Park, C. E.; Akhtar, M. Kalim; Rayman, K.

doi:10.1021/bk-1996-0621.ch020

Cited by 33 publications

(47 citation statements)

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“…(Valdivieso-Garcia et al 1996). The sensitivity was similar to that reported for three commercial kits for the detection of staphylococcal enterotoxin (Park et al 1992).…”

Section: Staphylococcus Aureussupporting

confidence: 82%

See 1 more Smart Citation

A review of analytical methods for the detection of bacterial toxins

Pimbley

Patel

1998

Journal of Applied Microbiology

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“…(Valdivieso-Garcia et al 1996). The sensitivity was similar to that reported for three commercial kits for the detection of staphylococcal enterotoxin (Park et al 1992).…”

Section: Staphylococcus Aureussupporting

confidence: 82%

“…The peroxidase was removed with sodium azide, but this reduced the recovery of enterotoxin by 20-30% (Park et al 1992). A collaborative study found sensitivities of 1 ng g −1 for ham, cheese, salami and turkey and 2·0 ng g −1 for other samples, with no false negative results.…”

Section: Staphylococcus Aureusmentioning

confidence: 99%

A review of analytical methods for the detection of bacterial toxins

Pimbley

Patel

1998

Journal of Applied Microbiology

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“…Detection and measurement of SEs has been performed in clinical situations by reverse passive latex agglutination (RPLA) (24,34,38,40,48) or ELISA (1,(35)(36)(37). However, these systems are hampered by either the long time required for determination of the responsible toxin or the low sensitivity to SE.…”

Section: Discussionmentioning

confidence: 99%

Establishment of Highly Specific and Quantitative Immunoassay Systems for Staphylococcal Enterotoxin A, B, and C Using Newly‐Developed Monoclonal Antibodies

Sasaki

Terano

Shibata

et al. 2005

Microbiology and Immunology

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Staphylococcal enterotoxin (SE) activities remain after boiling or treating with proteases. The main symptoms such as vomiting and diarrhea, are caused by the ingestion of SEs. Among SEs, SEA has been reported to be the major and most toxic protein. A highly specific and simple assay system is required to diagnose staphylococcal food poisoning. Therefore, the development of a suitable assay system is strongly anticipated. In this study, we have established a highly specific and sensitive avidin‐biotin sandwich ELISA (ABS‐ELISA) system for SEA, SEB, and SEC1 using newly‐developed monoclonal antibodies. The linearity of these systems obtained was in the range of 0.78–25 ng/ml for each SE, and furthermore, the lower concentrations of SEs could also be detected. The recoveries of SEs from murine serum, skim milk solution, and raw milk were found to be over 90%, suggesting that our systems could detect SEs without any interventions, such as these from milk or serum proteins. We were also able to quantify SEs in 22 specimens of culture supernatants of S. aureus isolated in past occurrences. Our established system should be very useful not only in the clinical field but also in various fields of investigation because of its quantification and simplicity in detecting SEs.

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“…As intoxicações estafilocócicas ocorrem devido a ingestão de toxina pré formada no alimento, e são frequentemente associadas à ingestão de 100 ng de enterotoxina (EE) e contagens entre 10 5 -10 6 UFC de estafilococos/ g ou mL de alimento BERGDOLL, 2002;LANCETTE;TATINI, 1992;PARK;AKTAR;RAYMAN, 1992) O homem e os animais são os principais reservatórios de S. aureus, sendo a cavidade nasal do homem seu principal habitat BERGDOLL, 2002;LANDGRAF, 1996). O S. aureus é também um dos principais agentes da mastite bovina, contaminando o leite na sua obtenção .…”

Section: Introductionunclassified

Estafilococos: morfologia das colônias, produção de coagulase e enterotoxina a, em amostras isoladas de leite cru refrigerado

et al. 2006

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ResumoOs relatos de intoxicação estafilocócica, principalmente por S. aureus, são frequentemente associados à ingestão de 100 ng de enterotoxina/g ou mL de alimento. A produção de enterotoxina pelos estafilococos está associada fortemente à produção de coagulase. Neste trabalho foram avaliadas amostras de leite de 51 propriedades leiteiras em Londrina (RS) e 50 em Pelotas. Todas as 101 amostras apresentaram contagens de estafilococos coagulase positivos (ECP). Das 552 colônias avaliadas, 256 foram coagulase positivas e destas cerca de 60% eram típicas no àgar Baird Parker e, em média, 60% do total de 296 estafilococos coagulase negativos (ECN) eram colônias atípicas. Quanto à capacidade enterotoxigênica, testou-se 109 colônias ECP, sendo 5,5% (6) positivas e 18 ECN, sendo 1 (5,5%) positiva. Assim, a freqüência de ECN produtores de EEA (5,5%) foi semelhante a dos ECP. Conclui-se que há extensa contaminação do leite cru por ECP, sendo grande parte (40%) das colônias atípicas produtoras de coagulase. Palavras-chave: Leite, estafilococos, toxinfecção, coagulase AbstractThe stories of food poisoning from staphylococci, mainly by S. aureus, are frequently associates to the ingestion of food with 100 ng of enterotoxin/g or mL. The production of enterotoxin using staphylococci is strongly associated with the production of coagulase. In this paper, milk samples of 51 milk properties in Londrina (RS) and 50 in Pelotas had been evaluated. All the 101 samples had presented counts of coagulase positive staphylococci (CPS). From 552 colonies studied, 256 were CPS where 60% of the colonies were typical in Baird Parker agar and, from 296 coagulase negative staphylococcus (CNS), in mean, 60% were atypical colonies. Due to the enterotoxigenic capacity, were tested 109 CPS, being 5,5% (6) positive and 18 CNS where 1 (5,5%) were positive. The frequency of CPS EEA (5,5%) producer was similar of the CNS. There is a wide milk contamination with CPS, where, a big part (40%) of the atypical colonies is coagulase producers.

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Efficacy of a Commercial Enzyme Immunoassay Kit for the Detection of Staphylococcal Enterotoxins in Foods

Cited by 33 publications

References 0 publications

A review of analytical methods for the detection of bacterial toxins

A review of analytical methods for the detection of bacterial toxins

Establishment of Highly Specific and Quantitative Immunoassay Systems for Staphylococcal Enterotoxin A, B, and C Using Newly‐Developed Monoclonal Antibodies

Estafilococos: morfologia das colônias, produção de coagulase e enterotoxina a, em amostras isoladas de leite cru refrigerado

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