Efficacy of a live Escherichia coli vaccine for protection of turkeys against homologous and heterologous field strains infection

Ahmed, Hanan; Ali, Abdel Hakim M.; Baky, Mansour H. Abdel

doi:10.21608/bvmj.2015.31539

Cited by 3 publications

(2 citation statements)

References 13 publications

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“…Therefore, studies have focused on using nutritional supplements such as omega-3 polyunsaturated fatty acids, vitamin E [8], phosphorus [9], fish oil [10], Bacillus subtilis [11], medicinal mushrooms [12], calcium [13], commercial organic acid [14], crude extract of Peganum harmala [15], and amla extract [16], to boost the immune system of poultry against bacterial infections [12]. Furthermore, bacterial vaccines are used to protect broilers from bacterial infections [17], such as autogenous E. coli vaccine [18], aroA-deleted vaccine [19], live E. coli vaccine [20], and…”

Section: Introductionmentioning

confidence: 99%

Effects of embryonic thermal manipulation on the immune response to post-hatch Escherichia coli challenge in broiler chicken

et al. 2023

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Background and Aim: Thermal manipulation (TM), exposure to mild heat shock during embryogenesis, which is a critical developmental period of broiler chickens, improves tissue stability, oxidative stress response, and immune response during heat stress. Thermal manipulation could be more cost-effective than other methods to boost the immune response. This study aimed to evaluate the impact of TM during embryogenesis, concomitant with an Escherichia coli challenge, on body weight (BW), body temperature (Tb), and splenic mRNA expression of cytokines (Interleukin [IL]-1β, IL-2, IL-6, IL-8, IL-12, IL-15, IL-16, IL-18, and interferon [IFN]-γ) in poultry. Materials and Methods: A total of 740 fertile eggs were procured from a certified Ross broiler breeder. The eggs were divided into two incubation groups: the control and TM groups. The eggs in the control group were kept at 37.8°C air temperature and 56% relative humidity (RH) during incubation; eggs of the TM group were incubated under standard conditions, except for embryonic days 10–18, during which they were incubated at 39°C and 65% RH for 18 h daily. On the 7th day of incubation, eggs with dead embryos were excluded. After hatching was complete, each group was further subdivided into saline-treated or E. coli-challenged groups. The E. coli (serotype 078 with the dose of 1.5 × 105 colony-forming unit/mL) challenge was performed when the birds were 20 days old. Body weight and Tb measurements were taken on post-hatch days 20, 21, 23, and 25. Splenic mRNA expression of cytokines (IL-1β, IL-2, IL-6, IL-8, IL-12, IL-15, IL-16, IL-18, and IFN-γ) was analyzed by real-time quantitative polymerase chain reaction. Results: Following the E. coli challenge, the TM-treated group’s body performance parameters (BW and Tb) were significantly increased compared with the control group. Body weight was higher in the TM group than in the control group (p < 0.05); Tb was lower in the TM group than in the control group (p < 0.05). The mRNA levels of IL and IFN-γ were more stable and moderately induced in the TM group compared with the control group. Thermal manipulation altered the basal mRNA levels of ILs and IFN-γ and changed their expression dynamics after the E. coli challenge. Conclusion: Thermal manipulation during embryogenesis could boost the immune system response to E. coli. Keywords: broiler, challenge, Escherichia coli, immune response, incubation, thermal manipulation.

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Section: Introductionmentioning

confidence: 99%

Effects of embryonic thermal manipulation on the immune response to post-hatch Escherichia coli challenge in broiler chicken

et al. 2023

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“…The increase of antibiotics resistance to E. coli and residues in meat should be the concern (Gregersen et al, 2010). Whereas E. coli vaccination provides a better result if the vaccine serotypes are homolog with field strain (Ahmed et al, 2015;Dho-Moulin and Fairbrother, 1999;La Ragione et al, 2013). In addition, there is no diagnostic tool to differentiate the E. coli isolates from primary (highly virulent), secondary (moderately secondary), or non-pathogenic (avirulent) isolates (Wooley et al, 2000;Oh et al, 2012).…”

Section: Introductionmentioning

confidence: 99%

Methionine Hydroxy Analog Supplementation to Increase Feed Utilization for Indigenous Sheep

Prasetiyono

Mulyono

Widiyanto

2020

Jurnal Sain Veteriner

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In the tropical area, productivity of ruminant has not optimized caused by the low quality of nutrition that leads to low-efficiency metabolism at the level of ruminal fermentation, post rumen digestibility, and intermediary metabolism. The study aimed to analyze effect of methionine hydroxy analog (MHA) on ruminal fermentation profiles of indigenous sheep specifically in the increase of ruminant productivity. In vitro utility test was conducted using rumen fluid of the indigenous sheep and sample of rational ration having a proportion of grass and concentrate 30%:70%, dry matter basis. The treatments implemented were three levels of MHA supplementation; T0: 0 g/day, T1: 3 g/day, and T2: 6 g/day. Variables measured were dry matter digestibility (DMD), organic matter digestibility (OMD), production of VFA, NH3, as well as total protein, and molar proportion of partial VFA of rumen fluid. Data were analyzed using analysis of variance (ANOVA) in a completely randomized design (CRD). The 0.2% MHA supplementation increased OMD with the highest production of total protein was from 28.57 mg/g (T0) to 40.49 mg/g (T2) (P<0.05). Meanwhile, the lowest ratio of acetate : propionate was from 2.74 (T0) to 2.33 (T2) (P<0.05). Supplementation of MHA up to 6 g/day concentrate increased the performance of fermentation and/or feed utility.

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Virulence Characteristic of Avian Pathogenic Escherichia coli (APEC) Isolates

Adrenalin

Imanjati

Fauziah

et al. 2020

Jurnal Sain Veteriner

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Avian pathogenic Escherichia coli (APEC) is a cause of colibacillosis in poultry, one of the respiratory disease that causes serious problems in the poultry industry. The APEC can cause high mortality and culling, decreased production, and high costs of treatment. Manifestations of colibacillosis are airsacculitis, perihepatitis, and pericarditis. The APEC serotypes that are widely identified in the field are O1K1, O2K1, and O78K80. Embryo lethality assay (ELA) is a method for determine the virulence of APEC serotypes. The aim of this study was to determine the virulence characteristic of APEC isolates. Five APEC serotypes O1K1, O2K1, O78K80, O157H7, and unknown serotype were used for ELA method by inoculated E. coli into chorioallantoic of specific pathogen free 12-days old embryos. Each group of 10 embryos, inoculated E. coli dose of 100-500 CFU/ 0,1 ml. Candling was carried out for 6 days (18-days old embryo) to determined the mortality and pathological lesions. The percentage of embryo mortality post-inoculated with APEC O1K1, O2K1, unknown serotypes were 100% (10/10), O78K80 serotype was 90% (9/10), and O157H7 serotype was 70% (70%). Lesions of all embryos were cranial and extremity hemorrhage. In this study, E. coli isolates had high virulence.

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Efficacy of a live Escherichia coli vaccine for protection of turkeys against homologous and heterologous field strains infection

Cited by 3 publications

References 13 publications

Effects of embryonic thermal manipulation on the immune response to post-hatch Escherichia coli challenge in broiler chicken

Effects of embryonic thermal manipulation on the immune response to post-hatch Escherichia coli challenge in broiler chicken

Methionine Hydroxy Analog Supplementation to Increase Feed Utilization for Indigenous Sheep

Virulence Characteristic of Avian Pathogenic Escherichia coli (APEC) Isolates

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